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[188648_at] Glutamine synthetase

Description C45B2.5 /REP_DB=WormBase Gene ID /WP=CE27854 /GB=AAK68249.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Glutamine synthetase
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 0      4grp_high_8_8 > 44      6grp_highest_nonorm > 50      6grp_total > 06      4grp_total > 44      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 250
1 2 3 4 range
A 517.9
(-1)
P 		622.4
(3)
P 		562.5
(0.7)
P 		466.4
(-3)
P 		156
B 654.6
(1.5)
P 		716.4
(3)
P 		546.8
(-1.2)
P 		474.8
(-3)
P 		242
C 602.7
(0.8)
P 		658.3
(3)
P 		539.6
(-1.6)
P 		504.7
(-3)
P 		154
avr 591.7
(0.6) 		665.7
(3) 		549.6
(-0.8) 		482
(-3) 		184

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 729
1 2 3 4 5 6 range
A 995.7
(3)
P 		659.2
(0.1)
P 		451.6
(-1.6)
P 		329.1
(-2.7)
P 		293.2
(-3)
P 		334.5
(-2.6)
P 		703
B 953.3
(3)
P 		526.9
(-0.7)
P 		450.1
(-1.4)
P 		391.7
(-1.9)
P 		388.4
(-1.9)
P 		267.2
(-3)
P 		686
C 911.8
(3)
P 		648.5
(0.4)
P 		338.5
(-2.6)
P 		338
(-2.6)
P 		301.1
(-2.9)
P 		293.7
(-3)
P 		618
avr 953.6
(3) 		611.5
(-0.1) 		413.4
(-1.9) 		352.9
(-2.5) 		327.6
(-2.7) 		298.5
(-3) 		655
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 140
1 2 3 4 range
A 188.6
(3)
P 		135.1
(0.3)
P 		147.1
(0.9)
P 		68.3
(-3)
P 		120
B 208.7
(3)
P 		176.9
(1.2)
P 		126.7
(-1.6)
P 		101.2
(-3)
P 		108
C 199.5
(3)
P 		189.3
(2.5)
P 		188.1
(2.4)
P 		85.1
(-3)
P 		114
avr 198.9
(3) 		167.1
(1.3) 		154
(0.6) 		84.9
(-3) 		114

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 270
1 2 3 4 5 6 range
A 183.6
(1)
P 		234.4
(3)
P 		98.3
(-2.3)
P 		83.5
(-2.9)
P 		88.5
(-2.7)
P 		81.4
(-3)
P 		153
B 331.7
(3)
P 		175.4
(-1)
P 		109.3
(-2.7)
P 		103.7
(-2.9)
P 		157.8
(-1.5)
P 		99.6
(-3)
P 		232
C 338
(3)
P 		197.9
(-0.1)
P 		86.1
(-2.6)
P 		68
(-3)
P 		97.4
(-2.3)
P 		98.3
(-2.3)
P 		270
avr 284.4
(3) 		202.6
(0.5) 		97.9
(-2.6) 		85.1
(-3) 		114.6
(-2.1) 		93.1
(-2.8) 		199
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188648_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16450
Sequence Type Exemplar sequence
Representative Public ID C45B2.5 NCBI
Target Description C45B2.5 /REP_DB=WormBase Gene ID /WP=CE27854 /GB=AAK68249.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Glutamine synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076611(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076611 NCBI Caenorhabditis elegans GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-1) (gln-1) mRNA, complete cds. 11/11 None
GENEFINDER00000012829 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6058240:6060017:1 11/11 None
SNAP00000012817 ENSEMBL cdna:SNAP chromosome:CEL140:X:6058385:6060017:1 11/11 None
C45B2.5.1 ENSEMBL cdna:known chromosome:CEL140:X:6058212:6060189:1 gene:C45B2.5 11/11 A 
C45B2.5.2 ENSEMBL cdna:known chromosome:CEL140:X:6058212:6060136:1 gene:C45B2.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6058239-6060017(+) 100.0 100.0
Public Domain and Genome References
Gene Title Glutamine synthetase
Gene Symbol gln-1
Ensembl C45B2.5
Entrez Gene 180875 Entrez gene
SwissProt Q95YD1 EMBL-EBI
WormBase CE27854 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
9399 nitrogen fixation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4356 glutamate-ammonia ligase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68249 Glutamine synthetase (glutamate-ammonia ligase) protein 1 [Caenorhabditis elegans] ref|NP_509012.1| GLutamiNe synthetase (glutamate-ammonia ligase) family member (gln-1) [Caenorhabditis elegans] 0.0
blast CAE68858 Hypothetical protein CBG14820 [Caenorhabditis briggsae] 0.0
blast CAE68858 Hypothetical protein CBG14820 [Caenorhabditis briggsae] 1.0E-175
Protein Domains
Database ID Description E-Value
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 5.1E-25
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 9.0E-74
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 8.8E-79
Pfam IPR008147 EMBL-EBI Glutamine synthetase, beta-Grasp domain 4.8E-22
Pfam IPR008146 EMBL-EBI Glutamine synthetase, catalytic domain 8.8E-79
Sequence
Target Sequence 
>C. ELEGANS:188648_AT
tcggatcgagttttcggtcggcacattcttgaagcacactacagagcctgcatgtacgcg
ggaatcaaaattagtggatctaatgtggagagtatgccatcacagtttgaatatcaagtt
ggtccatgcgaaggtattgagatgggagatcaactgtgggtgtctcgatacattctgcac
cgcatttgtgaagactacggagttgtatgtagtttggaccctaaaccagtgctcggtgaa
tggagcggtgctggatgtcatctcaacttctccacaaacgtgatgaggacaccatgcgaa
gatggaacagggtggaaagctatcgaggaagcagtcgtcaagctgagcaaagtacatatg
catcatattgcgtattatgatcctcacggaggacgtgataatgagcgccgacttattgga
gctaatcagactgaaactgttgacgcattttcctctggagttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCGGATCGAGTTTTCGGTCGGCACA 710 605 526 Antisense
GGTCGGCACATTCTTGAAGCACACT 69 499 541 Antisense
GAAGCACACTACAGAGCCTGCATGT 294 341 556 Antisense
AGCCTGCATGTACGCGGGAATCAAA 604 81 570 Antisense
GTGGGTGTCTCGATACATTCTGCAC 286 489 681 Antisense
GACCCTAAACCAGTGCTCGGTGAAT 432 379 742 Antisense
TGGAGCGGTGCTGGATGTCATCTCA 702 547 766 Antisense
ATCTCAACTTCTCCACAAACGTGAT 532 29 785 Antisense
GGAGGACGTGATAATGAGCGCCGAC 624 515 913 Antisense
GAGCGCCGACTTATTGGAGCTAATC 356 383 928 Antisense
TTGACGCATTTTCCTCTGGAGTTGC 284 703 965 Antisense
Sequence Source Affymetrix Proprietary Database