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[188643_s_at]

Description C28H8.12 /REP_DB=WormBase Gene ID /WP=CE01823 /GEN=dnc-2 /TR=SW:Q09248 /GB=AAA62300.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 16      6grp_high1_10_10 > 50      4grp_highest_8_8 > 25      4grp_high_8_8 > 59      6grp_highest_nonorm > 66      6grp_total > 51      4grp_total > 60      6grp_highest_sub > 03_04      6grp_cgc > 93      6grp_high1_sub > 03_04      6grp_cgc_sub > 03      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 622
1 2 3 4 range
A 1394.8
(2.8)
P 		1408.8
(3)
P 		1341.6
(2)
P 		1022.5
(-3)
P 		386
B 1411.6
(2.2)
P 		1482
(3)
P 		1437.5
(2.5)
P 		952.9
(-3)
P 		529
C 1324.3
(1.5)
P 		1258.1
(0.8)
P 		1482.5
(3)
P 		861
(-3)
P 		622
avr 1376.9
(2.4) 		1383
(2.5) 		1420.5
(3) 		945.5
(-3) 		475

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 816
1 2 3 4 5 6 range
A 1740.7
(0.4)
P 		1359.3
(-2.4)
P 		2044.9
(2.7)
P 		2092.5
(3)
P 		1328.7
(-2.6)
P 		1276.3
(-3)
P 		816
B 1775.6
(0.7)
P 		1556.2
(-1.2)
P 		2045.4
(3)
P 		1683.7
(-0.1)
P 		1692.3
(-0)
P 		1344.4
(-3)
P 		701
C 1562.5
(-0.7)
P 		1591.2
(-0.4)
P 		1714.2
(0.8)
P 		1925.1
(3)
P 		1337.1
(-3)
P 		1351
(-2.9)
P 		588
avr 1692.9
(0.6) 		1502.2
(-1.2) 		1934.8
(3) 		1900.4
(2.7) 		1452.7
(-1.7) 		1323.9
(-3) 		611
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 372
1 2 3 4 range
A 507.8
(3)
P 		305.8
(-0.4)
P 		350.7
(0.4)
P 		149.7
(-3)
P 		358
B 450.1
(3)
P 		366
(1)
P 		333.1
(0.2)
P 		203.1
(-3)
P 		247
C 438.4
(1.7)
P 		361.7
(0.5)
P 		516.7
(3)
P 		145.2
(-3)
P 		372
avr 465.4
(3) 		344.5
(0.6) 		400.2
(1.7) 		166
(-3) 		299

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 377
1 2 3 4 5 6 range
A 320.9
(-2.7)
P 		483.4
(1.7)
P 		445.3
(0.7)
P 		530.7
(3)
P 		400.9
(-0.5)
P 		310.7
(-3)
P 		220
B 617.8
(1.3)
P 		518
(-1.2)
P 		496.7
(-1.7)
P 		446
(-3)
P 		687.6
(3)
P 		501.3
(-1.6)
P 		242
C 579.1
(3)
P 		485.5
(0.1)
P 		436.3
(-1.5)
P 		387.1
(-3)
P 		432.7
(-1.6)
P 		452.3
(-1)
P 		192
avr 505.9
(2.9) 		495.6
(2.2) 		459.4
(-0.3) 		454.6
(-0.7) 		507.1
(3) 		421.4
(-3) 		86
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188643_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8026
Sequence Type Exemplar sequence
Representative Public ID C28H8.12 NCBI
Target Description C28H8.12 /REP_DB=WormBase Gene ID /WP=CE01823 /GEN=dnc-2 /TR=SW:Q09248 /GB=AAA62300.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065885(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065885 NCBI Caenorhabditis elegans DyNactin Complex component family member (dnc-2) (dnc-2) mRNA, complete cds. 11/11 None
SNAP00000005438 ENSEMBL cdna:SNAP chromosome:CEL140:III:5924477:5925680:-1 11/11 None
GENEFINDER00000005452 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5924477:5925680:-1 11/11 None
C28H8.12 ENSEMBL cdna:known chromosome:CEL140:III:5924367:5925703:-1 gene:C28H8.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5924475-5925679(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dnc-2
Ensembl C28H8.12
Entrez Gene 175837 Entrez gene
SwissProt Q09248 EMBL-EBI
WormBase CE01823 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7017 microtubule-based process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5869 dynactin complex inferred from electronic annotation QuickGO AmiGO
5869 dynactin complex inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_498286 DyNactin Complex component family member (dnc-2) [Caenorhabditis elegans] gb|AAA62300.1| Dynactin complex component protein 2 [Caenorhabditis elegans] sp|Q09248|YP9C_CAEEL Hypothetical protein C28H8.12 in chromosome III 0.0
blast NP_498286 DyNactin Complex component family member (dnc-2) [Caenorhabditis elegans] gb|AAA62300.1| Dynactin complex component protein 2 [Caenorhabditis elegans] sp|Q09248|YP9C_CAEEL Hypothetical protein C28H8.12 in chromosome III 1.0E-177
blast CAE64361 Hypothetical protein CBG09048 [Caenorhabditis briggsae] 1.0E-160
blast CAE64361 Hypothetical protein CBG09048 [Caenorhabditis briggsae] 1.0E-156
blast AAH53120 Similar to dynactin 2 (p50) [Danio rerio] ref|NP_957460.1| dynactin 2 (p50) [Danio rerio] 3.0E-22
Protein Domains
Database ID Description E-Value
Pfam IPR006996 EMBL-EBI Dynamitin 1.0E-126
Pfam IPR006996 EMBL-EBI Dynamitin 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188643_S_AT
gaaaacgttgcaagtggctcagtcaaatggaaaaactgcacgagttgagcatgatgttga
gttaccaaatgttcgcactgactcgaaagtggcaactcttgaaaataggttgagaagaat
cgaacaagttattggaagttcggttattccatcagctcccgtacttgacaccattgaaga
tctcaaactcagatgcgaaacgcttaatcattcgtatgtctccggtcttgaacaacgttt
gaatatgatgcttaccaaacttgaaaaaatcgacgagacacgcgccaacaatgatatcga
cgagaatcttgacaagaaagttgatgaaattctggagcttatgcaaaaatgggatgtcgc
ttgctcatctcttccaagcaatgttaataaggttaaagcattgaaccgtcttcacgaaca
agttcaacactttgccggtggacta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAACGTTGCAAGTGGCTCAGTCA 119 355 411 Antisense
GTTACCAAATGTTCGCACTGACTCG 3 447 471 Antisense
GGAAGTTCGGTTATTCCATCAGCTC 695 529 544 Antisense
CTCCCGTACTTGACACCATTGAAGA 189 235 566 Antisense
TAATCATTCGTATGTCTCCGGTCTT 507 635 615 Antisense
GTCTCCGGTCTTGAACAACGTTTGA 220 465 628 Antisense
AAAAATCGACGAGACACGCGCCAAC 663 127 675 Antisense
GACACGCGCCAACAATGATATCGAC 570 367 687 Antisense
AAATGGGATGTCGCTTGCTCATCTC 633 119 757 Antisense
AAGCATTGAACCGTCTTCACGAACA 359 149 806 Antisense
AGTTCAACACTTTGCCGGTGGACTA 655 61 831 Antisense
Sequence Source Affymetrix Proprietary Database