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[188642_s_at] trehalase

Description W05E10.4 /REP_DB=WormBase Gene ID /WP=CE06551 /TR=Q23176 /GB=CAB01250.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=trehalase
Cluster 6grp_highest_10_10 > 34      6grp_high1_10_10 > 33      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 52      6grp_total > 31      4grp_total > 55      6grp_highest_sub > 02_03      6grp_cgc > 73      6grp_high1_sub > 02      6grp_cgc_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 562
1 2 3 4 range
A 804.5
(3)
P 		377.1
(-1.6)
P 		474.9
(-0.5)
P 		242.3
(-3)
P 		562
B 793.8
(3)
P 		326.8
(-2.8)
P 		534.7
(-0.2)
P 		311.4
(-3)
P 		482
C 705.3
(3)
P 		405.1
(-1.2)
P 		450.2
(-0.5)
P 		272
(-3)
P 		433
avr 767.9
(3) 		369.7
(-1.8) 		486.6
(-0.4) 		275.2
(-3) 		493

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 617
1 2 3 4 5 6 range
A 499.6
(-1)
P 		748.8
(2.3)
P 		668.6
(1.2)
P 		803.4
(3)
P 		534.3
(-0.6)
P 		349.6
(-3)
P 		454
B 640.6
(0.6)
P 		803.3
(2.6)
P 		836.7
(3)
P 		669
(1)
P 		795.3
(2.5)
P 		341
(-3)
P 		496
C 636.2
(-0.2)
P 		957.5
(3)
P 		721.2
(0.6)
P 		660.7
(0)
P 		580.4
(-0.8)
P 		354.8
(-3)
P 		603
avr 592.1
(-0) 		836.5
(3) 		742.2
(1.8) 		711
(1.5) 		636.7
(0.5) 		348.5
(-3) 		488
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 257
1 2 3 4 range
A 292.9
(3)
P 		81.9
(-1.9)
P 		124.2
(-0.9)
P 		35.5
(-3)
P 		257
B 253.1
(3)
P 		80.7
(-2.5)
P 		123.9
(-1.2)
P 		66.4
(-3)
P 		187
C 233.5
(3)
P 		116.5
(-0.7)
P 		156.9
(0.6)
P 		45.9
(-3)
P 		188
avr 259.8
(3) 		93
(-1.8) 		135
(-0.6) 		49.3
(-3) 		211

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 92.1
(-2.8)
P 		266.3
(3)
P 		145.6
(-1)
P 		203.8
(0.9)
P 		161.2
(-0.5)
P 		85.1
(-3)
P 		181
B 222.9
(-0.1)
P 		267.4
(1.3)
P 		203.2
(-0.7)
P 		177.2
(-1.5)
P 		323.1
(3)
P 		127.1
(-3)
P 		196
C 235.8
(1)
P 		292.2
(3)
P 		183.5
(-0.8)
P 		132.9
(-2.5)
P 		187.8
(-0.6)
P 		118.8
(-3)
P 		173
avr 183.6
(-0.3) 		275.3
(3) 		177.4
(-0.6) 		171.3
(-0.8) 		224
(1.1) 		110.3
(-3) 		165
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188642_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13846
Sequence Type Exemplar sequence
Representative Public ID W05E10.4 NCBI
Target Description W05E10.4 /REP_DB=WormBase Gene ID /WP=CE06551 /TR=Q23176 /GB=CAB01250.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=trehalase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073558 NCBI Caenorhabditis elegans W05E10.4 (trehalase) mRNA, complete cds. 11/11 A 
GENEFINDER00000019395 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:11704868:11718420:-1 11/11 None
W05E10.4 ENSEMBL cdna:known chromosome:CEL140:V:11711920:11718492:-1 gene:W05E10.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000019390 1/11 Cross Hyb Matching Probes None
SNAP00000019391 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:11712426-11718420(-) 99.21 100.0
Public Domain and Genome References
Gene Title trehalase
Gene Symbol tre-3
Ensembl W05E10.4
Entrez Gene 179604 Entrez gene
SwissProt Q23176 EMBL-EBI
WormBase CE06551 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5991 trehalose metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4555 alpha,alpha-trehalase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01250 Hypothetical protein W05E10.4 [Caenorhabditis elegans] ref|NP_505959.1| W05E10.4 [Caenorhabditis elegans] emb|CAD54509.1| trehalase [Caenorhabditis elegans] 0.0
blast CAE75311 Hypothetical protein CBG23281 [Caenorhabditis briggsae] 0.0
blast CAB01249 Hypothetical protein W05E10.3 [Caenorhabditis elegans] ref|NP_505958.1| C.Elegans Homeobox family member (ceh-32) [Caenorhabditis elegans] gb|AAG49583.1| CEH-32 [Caenorhabditis elegans] sp|Q23175|HM32_CAEEL Homeobox protein ceh-32 0.0
blast AAK31441 Trehalase protein 5 [Caenorhabditis elegans] ref|NP_493649.2| C23H3.7 [Caenorhabditis elegans] emb|CAD54512.1| trehalase [Caenorhabditis elegans] 1.0E-147
Protein Domains
Database ID Description E-Value
scop a.4.1.Homeodomain All alpha proteins; DNA/RNA-binding 3-helical bundle; Homeodomain-like; Homeodomain 6.00000021222513E-6
scop a.2.7.Valyl-tRNA synthetase (ValRS) C-terminal domain All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Valyl-tRNA synthetase (ValRS) C-terminal domain 2.59999990463257
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 1.0E-126
Pfam IPR001356 EMBL-EBI Homeobox 4.4E-10
Pfam IPR001661 EMBL-EBI Glycoside hydrolase, family 37 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188642_S_AT
gaacagatggaacttggtacgattataatttgagaacacaatcccataatccaagattct
acacaagtaccgcagttcctttattcacaaattgctataacacattgaataccggaaaat
cacaaaaagtattcgactatatggataaaatgggagtattcacttatccagggggaattc
caacttcaatgtctcaagaaagtgaccaacaatgggatttcccaaatggatggagtccaa
ataatcatatgatcattgaaggattaagaaaatcagcaaatcccgaaatgcaagataagg
gattcctgattgcttccaaatgggttatgggcaacttccgagtgttctatgaaaccggcc
acatgtgggagaagtacaacgtgatcggcagctacccacaaccaggatctggaggtgaat
atgatgttcaggatggatttggatggagcaacggagctatattggatcttctcttgacat
ataacgatcgattatttgttccagaaaactttgtcaacacaactgtcacacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACAGATGGAACTTGGTACGATTA 70 351 1151 Antisense
GAGAACACAATCCCATAATCCAAGA 42 393 1182 Antisense
GATTCTACACAAGTACCGCAGTTCC 178 431 1205 Antisense
ACCGCAGTTCCTTTATTCACAAATT 302 89 1219 Antisense
GGGAGTATTCACTTATCCAGGGGGA 169 379 1302 Antisense
GGGGGAATTCCAACTTCAATGTCTC 574 497 1321 Antisense
AAGTGACCAACAATGGGATTTCCCA 119 159 1350 Antisense
GAAAATCAGCAAATCCCGAAATGCA 123 353 1418 Antisense
GGATTCCTGATTGCTTCCAAATGGG 118 505 1450 Antisense
ATGGGTTATGGGCAACTTCCGAGTG 626 49 1470 Antisense
CTTTGTCAACACAACTGTCACACCT 453 223 1659 Antisense
Sequence Source Affymetrix Proprietary Database