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[188635_at]

Description F58E6.1 /REP_DB=WormBase Gene ID /WP=CE25921 /CHR=5 /FEA=Sanger Annotation /DEF=SH2 domain (HINXTON) TR:Q20977 protein_id:CAA94771.2
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 92      4grp_high_8_8 > 46      6grp_highest_nonorm > 55      6grp_total > 83      4grp_total > 50      6grp_highest_sub > 04_05      6grp_high1_sub > 04_05      6grp_total_sub > Hat      6grp_rule_based > S5      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 268
1 2 3 4 range
A 121.4
(-3)
P 		385.3
(3)
P 		352.8
(2.3)
P 		142.4
(-2.5)
P 		264
B 190.2
(-1.7)
A 		385.5
(3)
P 		295.9
(0.8)
P 		138.5
(-3)
P 		247
C 153
(-2.1)
A 		352.1
(3)
P 		342
(2.7)
P 		117.9
(-3)
P 		234
avr 154.9
(-2.5) 		374.3
(3) 		330.2
(1.9) 		132.9
(-3) 		241

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2959
1 2 3 4 5 6 range
A 348.3
(-3)
P 		444.4
(-2.8)
P 		388.6
(-2.9)
P 		2913
(2.4)
P 		3174.6
(3)
P 		536.3
(-2.6)
P 		2826
B 344.1
(-3)
P 		410.8
(-2.8)
P 		322.8
(-3)
P 		2606.5
(1.7)
P 		3242.8
(3)
P 		445.4
(-2.7)
P 		2920
C 305
(-3)
P 		479.3
(-2.6)
P 		283.9
(-3)
P 		2631.1
(1.9)
P 		3168.1
(3)
P 		522.5
(-2.5)
P 		2884
avr 332.5
(-3) 		444.8
(-2.8) 		331.8
(-3) 		2716.9
(2) 		3195.2
(3) 		501.4
(-2.6) 		2863
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 44.2
(-1)
P 		83.6
(2.3)
P 		92.2
(3)
P 		20.8
(-3)
P 		71
B 60.7
(-0.2)
A 		95.2
(3)
P 		68.6
(0.6)
P 		29.5
(-3)
P 		66
C 50.6
(-1.1)
A 		101.2
(1.9)
P 		119.2
(3)
P 		19.9
(-3)
P 		99
avr 51.8
(-0.6) 		93.3
(3) 		93.3
(3) 		23.4
(-3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1253
1 2 3 4 5 6 range
A 64.2
(-3)
P 		158
(-2.4)
P 		84.6
(-2.9)
P 		738.8
(1.5)
P 		958
(3)
P 		130.6
(-2.6)
P 		894
B 119.7
(-2.8)
P 		136.7
(-2.7)
P 		78.4
(-3)
P 		690.4
(-0)
P 		1317.5
(3)
P 		166.1
(-2.6)
P 		1239
C 113
(-2.7)
P 		146.2
(-2.5)
P 		72.3
(-3)
P 		529.1
(-0.1)
P 		1025.1
(3)
P 		174.9
(-2.4)
P 		953
avr 99
(-2.9) 		147
(-2.6) 		78.4
(-3) 		652.8
(0.4) 		1100.2
(3) 		157.2
(-2.5) 		1022
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188635_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12453
Sequence Type Exemplar sequence
Representative Public ID F58E6.1 NCBI
Target Description F58E6.1 /REP_DB=WormBase Gene ID /WP=CE25921 /CHR=5 /FEA=Sanger Annotation /DEF=SH2 domain (HINXTON) TR:Q20977 protein_id:CAA94771.2
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028825(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028825 NCBI Caenorhabditis elegans F58E6.1a (F58E6.1) mRNA, complete cds. 11/11 None
SNAP00000040364 ENSEMBL cdna:SNAP chromosome:CEL140:V:9747823:9748829:1 11/11 None
GENEFINDER00000040378 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9747823:9748829:1 11/11 None
F58E6.1a ENSEMBL cdna:known chromosome:CEL140:V:9742298:9748858:1 gene:F58E6.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
3565291 2 1 NM_001028825 188635_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9742297-9748829(+) 100.0 100.0
Public Domain and Genome References
Ensembl F58E6.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA94771 Hypothetical protein F58E6.1a [Caenorhabditis elegans] ref|NP_001023996.1| F58E6.1a [Caenorhabditis elegans] 0.0
blast CAC42308 Hypothetical protein F58E6.1b [Caenorhabditis elegans] ref|NP_741599.1| F58E6.1b [Caenorhabditis elegans] 0.0
blast AAD45535 zinc finger protein STAT-B [Caenorhabditis elegans] 0.0
blast CAA94771 Hypothetical protein F58E6.1a [Caenorhabditis elegans] ref|NP_001023996.1| F58E6.1a [Caenorhabditis elegans] 1.0E-114
blast T22918 hypothetical protein F58E6.2 - Caenorhabditis elegans 1.0E-114
Protein Domains
Database ID Description E-Value
Pfam IPR001841 EMBL-EBI Zn-finger, RING 1.2E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023996.1 3 117-139,605-627,741-763
F58E6.1a 3 117-139,605-627,741-763
Sequence
Target Sequence 
>C. ELEGANS:188635_AT
ttgctttaataccgtgctttcttgcggttcacgtcgtaggagacagtaatgaagttaccg
ttccagctgtgagagttgtccgtcttcaagttgattacagaaatgcatctgtttcagatt
tgcagaagattcacaagtggaatgctatcatgcgaaatagtgtgcttgcatccctgaagt
ttatcaacaagcattggcttatttgtggaggatctccatctgatacaccaacttcttcaa
acgcagactgtggaaaagctcaagtgactggagaaattgttggagatagacattatagaa
tcaatgttactttgattgctgagagagatccagtcaaaaacgctaaagtcggagctacct
caacggtctacgcagttgctcatattggattaaagggaggaatcttccaatacacgaatg
ccctgaagactcttggaaaaccagaaccaaaactcgcattcgatgaagcattcttctgct
atcgcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCTTTAATACCGTGCTTTCTTGC 371 701 2315 Antisense
TTTCTTGCGGTTCACGTCGTAGGAG 480 443 2332 Antisense
AGTTACCGTTCCAGCTGTGAGAGTT 494 57 2367 Antisense
GAGAGTTGTCCGTCTTCAAGTTGAT 20 365 2385 Antisense
ATAGTGTGCTTGCATCCCTGAAGTT 657 19 2471 Antisense
TGGAGGATCTCCATCTGATACACCA 33 551 2520 Antisense
TACACCAACTTCTTCAAACGCAGAC 646 643 2538 Antisense
TAAAGTCGGAGCTACCTCAACGGTC 244 639 2658 Antisense
AACGGTCTACGCAGTTGCTCATATT 539 143 2676 Antisense
TACACGAATGCCCTGAAGACTCTTG 399 643 2725 Antisense
TGAAGCATTCTTCTGCTATCGCGGA 508 573 2778 Antisense
Sequence Source Affymetrix Proprietary Database