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[188625_at] MAF protein

Description C54G4.6 /REP_DB=WormBase Gene ID /WP=CE05512 /TR=Q18851 /GB=CAA99818.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=MAF protein
Cluster 6grp_highest_10_10 > 67      6grp_high1_10_10 > 76      4grp_highest_8_8 > 24      4grp_high_8_8 > 51      6grp_highest_nonorm > 82      6grp_total > 66      4grp_total > 60      6grp_highest_sub > In      6grp_cgc > 47      6grp_high1_sub > Da      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 276
1 2 3 4 range
A 300.9
(0.3)
P 		417.7
(3)
P 		336.7
(1.1)
P 		155.8
(-3)
P 		262
B 431.4
(3)
P 		379.5
(1.2)
P 		421.4
(2.6)
P 		261.4
(-3)
P 		170
C 398.2
(3)
P 		319.9
(-0.7)
P 		340
(0.3)
P 		269.8
(-3)
P 		128
avr 376.8
(3) 		372.4
(2.8) 		366
(2.6) 		229
(-3) 		148

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 257
1 2 3 4 5 6 range
A 307.4
(-3)
P 		345.5
(-1.5)
P 		464.8
(3)
P 		338.6
(-1.8)
P 		341
(-1.7)
P 		397.9
(0.4)
P 		157
B 310.1
(-2.4)
P 		397.5
(0.4)
P 		393.5
(0.3)
P 		290.2
(-3)
P 		355.7
(-0.9)
P 		476.9
(3)
P 		187
C 310.1
(-1.3)
P 		375.5
(0.2)
P 		287.3
(-1.9)
P 		238.9
(-3)
P 		362.7
(-0.1)
P 		495.9
(3)
P 		257
avr 309.2
(-2.3) 		372.8
(-0) 		381.9
(0.3) 		289.2
(-3) 		353.1
(-0.7) 		456.9
(3) 		168
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 115
1 2 3 4 range
A 109.6
(3)
P 		90.7
(1.7)
P 		88
(1.5)
P 		22.8
(-3)
P 		87
B 137.6
(3)
P 		93.7
(-0.2)
P 		97.6
(0.1)
P 		55.7
(-3)
P 		82
C 131.8
(3)
P 		92
(0.2)
P 		118.5
(2.1)
P 		45.5
(-3)
P 		86
avr 126.3
(3) 		92.1
(0.6) 		101.4
(1.2) 		41.3
(-3) 		85

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 56.7
(-3)
P 		122.9
(3)
P 		101.2
(1)
P 		85.9
(-0.4)
P 		102.9
(1.2)
P 		96.9
(0.6)
P 		66
B 107.9
(-1.2)
P 		132.3
(0.3)
P 		95.6
(-1.9)
P 		76.9
(-3)
P 		144.5
(1)
P 		177.8
(3)
P 		101
C 114.9
(0.4)
P 		114.6
(0.4)
P 		73.1
(-1.7)
P 		48
(-3)
P 		117.4
(0.5)
P 		166
(3)
P 		118
avr 93.2
(-1.2) 		123.3
(1.2) 		90
(-1.5) 		70.3
(-3) 		121.6
(1) 		146.9
(3) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188625_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.337
Sequence Type Exemplar sequence
Representative Public ID C54G4.6 NCBI
Target Description C54G4.6 /REP_DB=WormBase Gene ID /WP=CE05512 /TR=Q18851 /GB=CAA99818.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=MAF protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059804(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059804 NCBI Caenorhabditis elegans Downstream Of DAF-16 (regulated by DAF-16) family member (dod-18) (dod-18) mRNA, complete cds. 11/11 None
SNAP00000028900 ENSEMBL cdna:SNAP chromosome:CEL140:I:8027821:8028919:-1 11/11 None
GENEFINDER00000028913 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8027821:8028993:-1 10/11 None
C54G4.6 ENSEMBL cdna:known chromosome:CEL140:I:8027821:8028919:-1 gene:C54G4.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8027751-8028850(-) 100.0 100.0
Public Domain and Genome References
Gene Title MAF protein
Gene Symbol dod-18
Ensembl C54G4.6
Entrez Gene 183813 Entrez gene
SwissProt Q18851 EMBL-EBI
WormBase CE05512 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.17257.1.S1_AT similar to N-acetylserotonin O-methyltransferase-like protein (ASMTL) cfa
CANINE_2:CFAAFFX.23521.1.S1_AT similar to N-acetylserotonin O-methyltransferase-like protein (ASMTL) cfa
HG-FOCUS:36554_AT acetylserotonin O-methyltransferase-like hs
HG-U133A:36554_AT acetylserotonin O-methyltransferase-like hs
HG-U133A_2:36554_AT acetylserotonin O-methyltransferase-like hs
HG-U133_PLUS_2:36554_AT acetylserotonin O-methyltransferase-like hs
HG-U95AV2:36554_AT acetylserotonin O-methyltransferase-like hs
HG-U133_PLUS_2:209394_AT acetylserotonin O-methyltransferase-like hs
HG-U133A_2:209394_AT acetylserotonin O-methyltransferase-like hs
HG-U133A:209394_AT acetylserotonin O-methyltransferase-like hs
HG-U133A_2:36553_AT acetylserotonin O-methyltransferase-like hs
HG-FOCUS:36553_AT acetylserotonin O-methyltransferase-like hs
HG-U133_PLUS_2:36553_AT acetylserotonin O-methyltransferase-like hs
HG-U133A:36553_AT acetylserotonin O-methyltransferase-like hs
HG-U95AV2:36553_AT acetylserotonin O-methyltransferase-like hs
HU35KSUBA:RC_AA427398_AT acetylserotonin O-methyltransferase-like hs
U133_X3P:G12803374_3P_AT acetylserotonin O-methyltransferase-like hs
U133_X3P:4874084_3P_S_AT acetylserotonin O-methyltransferase-like hs
U133_X3P:209394_3P_AT acetylserotonin O-methyltransferase-like hs
HG-U95E:72369_AT acetylserotonin O-methyltransferase-like hs
Protein Similarities
Method ID Description E-Value
blast CAA99818 Hypothetical protein C54G4.6 [Caenorhabditis elegans] ref|NP_492205.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-18) [Caenorhabditis elegans] 1.0E-104
blast CAA99818 Hypothetical protein C54G4.6 [Caenorhabditis elegans] ref|NP_492205.1| Downstream Of DAF-16 (regulated by DAF-16) family member (dod-18) [Caenorhabditis elegans] 1.0E-91
blast CAE63695 Hypothetical protein CBG08210 [Caenorhabditis briggsae] 8.0E-86
blast CAE63695 Hypothetical protein CBG08210 [Caenorhabditis briggsae] 5.0E-82
blast EAA22330 maf protein [Plasmodium yoelii yoelii] 3.0E-31
blast NP_782638 septum formation protein maf [Clostridium tetani E88] gb|AAO36575.1| septum formation protein maf [Clostridium tetani E88] 2.0E-27
Protein Domains
Database ID Description E-Value
Pfam IPR003697 EMBL-EBI Maf-like protein 6.0E-53
Pfam IPR003697 EMBL-EBI Maf-like protein 5.4E-9
Pfam IPR003697 EMBL-EBI Maf-like protein 1.5E-49
Sequence
Target Sequence 
>C. ELEGANS:188625_AT
gatttatttcggtttcagggattggaaaatgtaatcgtaaaagtttcaaatttcgaggaa
aatctaccaaaaacattgccagttaagcagtttgtcattgaaacagctaaaggaaaattg
acgactattgtggaagaaatgaaaagaaaagaggagccgtactctgttattattgcgtgt
gatacagtaatcgaattcaatggagaaattattggaaaaccaacagatgccaatgacgct
aaagaaactttgaaaagacttcgtaacaatactcataatgtatatactggaatggctcta
cactatcatgaaactgatcaatatgaagagatcatcgagaaaacaattgttcattttgga
gatattcctgatagagttatcgacgaatatgtaaaaagcggagagccattgaaaaaagca
ggttcatatggaatcggtgactttggcgctgtcttcgttcgtggaatcgaaggatgtatg
ccgaatgtggttggtttgccacttcaccg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTATTTCGGTTTCAGGGATTGG 73 419 34 Antisense
GCCAGTTAAGCAGTTTGTCATTGAA 314 281 111 Antisense
GGAGCCGTACTCTGTTATTATTGCG 40 523 186 Antisense
AACAGATGCCAATGACGCTAAAGAA 77 135 255 Antisense
AAGACTTCGTAACAATACTCATAAT 467 147 288 Antisense
TATACTGGAATGGCTCTACACTATC 636 659 316 Antisense
TGGCTCTACACTATCATGAAACTGA 383 545 326 Antisense
AAAGCAGGTTCATATGGAATCGGTG 440 121 448 Antisense
TGGAATCGGTGACTTTGGCGCTGTC 14 547 462 Antisense
GGATGTATGCCGAATGTGGTTGGTT 186 515 505 Antisense
ATGTGGTTGGTTTGCCACTTCACCG 249 49 518 Antisense
Sequence Source Affymetrix Proprietary Database