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[188624_at]

Description F22B7.10 /REP_DB=WormBase Gene ID /WP=CE26462 /GEN=dpy-19 /TR=SW:P34413 /GB=AAA65466.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 12      4grp_highest_8_8 > 42      4grp_high_8_8 > 41      6grp_highest_nonorm > 60      6grp_total > 23      4grp_total > 54      6grp_highest_sub > De      6grp_cgc > 30      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 339
1 2 3 4 range
A 520.1
(3)
P 		283.8
(-1.4)
P 		288
(-1.4)
P 		200.1
(-3)
P 		320
B 361.9
(3)
P 		265.1
(-0.2)
P 		255.2
(-0.5)
P 		181
(-3)
P 		181
C 487.8
(3)
P 		307.3
(-0.8)
P 		310.2
(-0.8)
P 		205.5
(-3)
P 		282
avr 456.6
(3) 		285.4
(-0.9) 		284.5
(-1) 		195.5
(-3) 		261

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 316
1 2 3 4 5 6 range
A 490.7
(3)
P 		373.3
(0.8)
P 		489.9
(3)
P 		367.1
(0.7)
P 		271
(-1.2)
P 		174.3
(-3)
P 		316
B 470.9
(2.8)
P 		478.7
(3)
P 		363.6
(0.6)
P 		318.9
(-0.4)
P 		205.4
(-2.7)
P 		193.1
(-3)
P 		286
C 456.5
(3)
P 		420.7
(2.1)
P 		349.3
(0.4)
P 		341.3
(0.2)
P 		248.2
(-2.1)
P 		212.7
(-3)
P 		244
avr 472.7
(3) 		424.2
(2) 		400.9
(1.5) 		342.4
(0.2) 		241.5
(-2) 		193.4
(-3) 		279
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 160
1 2 3 4 range
A 189.4
(3)
P 		61.6
(-1.8)
P 		75.3
(-1.3)
P 		29.3
(-3)
P 		160
B 115.4
(3)
P 		65.5
(-0.9)
P 		59.1
(-1.4)
P 		38.6
(-3)
P 		77
C 161.5
(3)
P 		88.4
(-0.5)
P 		108.1
(0.5)
P 		34.6
(-3)
P 		127
avr 155.4
(3) 		71.8
(-1.1) 		80.8
(-0.7) 		34.2
(-3) 		121

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 127
1 2 3 4 5 6 range
A 90.5
(0.2)
P 		132.8
(3)
P 		106.7
(1.3)
P 		93.1
(0.4)
P 		81.8
(-0.4)
P 		42.4
(-3)
P 		90
B 163.9
(3)
P 		159.3
(2.7)
P 		88.3
(-1.9)
P 		84.5
(-2.2)
P 		83.4
(-2.3)
P 		72
(-3)
P 		92
C 169.2
(3)
P 		128.4
(0.6)
P 		88.9
(-1.8)
P 		68.6
(-3)
P 		80.3
(-2.3)
P 		71.2
(-2.8)
P 		101
avr 141.2
(3) 		140.2
(2.9) 		94.6
(-0.5) 		82.1
(-1.5) 		81.8
(-1.5) 		61.9
(-3) 		79
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188624_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7053
Sequence Type Exemplar sequence
Representative Public ID F22B7.10 NCBI
Target Description F22B7.10 /REP_DB=WormBase Gene ID /WP=CE26462 /GEN=dpy-19 /TR=SW:P34413 /GB=AAA65466.2 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066508(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066508 NCBI Caenorhabditis elegans DumPY : shorter than wild-type family member (dpy-19) (dpy-19) mRNA, complete cds. 11/11 None
GENEFINDER00000003841 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8659801:8662737:-1 11/11 None
SNAP00000003838 ENSEMBL cdna:SNAP chromosome:CEL140:III:8659870:8662645:-1 11/11 None
F22B7.10 ENSEMBL cdna:known chromosome:CEL140:III:8659870:8667377:-1 gene:F22B7.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8659864-8667372(-) 99.95 100.0
Public Domain and Genome References
Gene Title
Gene Symbol dpy-19
Ensembl F22B7.10
Entrez Gene 191628 Entrez gene
SwissProt P34413 EMBL-EBI
WormBase CE26462 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.5713.1.S1_AT similar to dpy-19-like 1, like cfa
CHICKEN:GGA.14646.1.A1_AT dpy-19-like 1 (C. elegans) gga
CHICKEN:GGA.2406.1.S1_AT dpy-19-like 1 (C. elegans) gga
CHICKEN:GGA.2406.2.S1_AT dpy-19-like 1 (C. elegans) gga
HU35KSUBA:AA258286_AT dpy-19-like 1 (C. elegans) hs
HU35KSUBA:RC_AA479212_AT dpy-19-like 1 (C. elegans) hs
HU35KSUBD:RC_AA432124_AT dpy-19-like 1 (C. elegans) hs
U133_X3P:HS2.11217.1.A1_3P_S_AT dpy-19-like 1 (C. elegans) hs
U133_X3P:HS2.11217.1.A1_3P_A_AT dpy-19-like 1 (C. elegans) hs
U133_X3P:HS.11217.0.A1_3P_AT dpy-19-like 1 (C. elegans) hs
HG-U133_PLUS_2:1560915_AT dpy-19-like 1 (C. elegans) hs
HG-U133_PLUS_2:1560916_A_AT dpy-19-like 1 (C. elegans) hs
HG-U133_PLUS_2:1570355_A_AT dpy-19-like 1 (C. elegans) hs
HG-U133A_2:212792_AT dpy-19-like 1 (C. elegans) hs
HG-U133_PLUS_2:212792_AT dpy-19-like 1 (C. elegans) hs
HG-U133A:212792_AT dpy-19-like 1 (C. elegans) hs
HG-U95AV2:33938_G_AT dpy-19-like 1 (C. elegans) hs
HG-U95AV2:39021_AT dpy-19-like 1 (C. elegans) hs
HU35KSUBC:RC_W87474_AT Dpy-19-like 1 (C. elegans) hs
U133_X3P:HS.58993.0.S1_3P_X_AT Dpy-19-like 1 (C. elegans) hs
U133_X3P:HS.58993.0.S1_3P_AT Dpy-19-like 1 (C. elegans) hs
HG-U133A_2:215433_AT Dpy-19-like 1 (C. elegans) hs
HG-U133_PLUS_2:215433_AT Dpy-19-like 1 (C. elegans) hs
HG-U133A:215433_AT Dpy-19-like 1 (C. elegans) hs
HG-U95AV2:33937_AT Dpy-19-like 1 (C. elegans) hs
MG-U74BV2:112379_AT dpy-19-like 1 (C. elegans) mm
MOE430B:1433759_AT dpy-19-like 1 (C. elegans) mm
MOUSE430_2:1433759_AT dpy-19-like 1 (C. elegans) mm
MOE430B:1456533_AT dpy-19-like 1 (C. elegans) mm
MOUSE430_2:1456533_AT dpy-19-like 1 (C. elegans) mm
MU19KSUBB:TC30951_AT Dpy-19-like 1 (C. elegans) (Dpy19l1), mRNA mm
RAE230A:1375174_AT Similar to KIAA0877 protein (predicted) rn
RAT230_2:1375174_AT Similar to KIAA0877 protein (predicted) rn
RAE230A:1389483_AT Similar to KIAA0877 protein (predicted) rn
RAT230_2:1389483_AT Similar to KIAA0877 protein (predicted) rn
RG-U34A:RC_AA892483_AT Similar to KIAA0877 protein (predicted) rn
RG-U34B:RC_AI070275_AT Similar to KIAA0877 protein (predicted) rn
Protein Similarities
Method ID Description E-Value
blast AAA65466 Dumpy : shorter than wild-type protein 19 [Caenorhabditis elegans] ref|NP_498909.1| DumPY : shorter than wild-type family member (dpy-19) [Caenorhabditis elegans] sp|P34413|DPY19_CAEEL Protein dpy-19 (Dumpy-19 protein) 0.0
blast CAE75029 Hypothetical protein CBG22936 [Caenorhabditis briggsae] 0.0
blast AAT68046 KIAA0877-like [Danio rerio] ref|NP_001002868.1| dpy-19-like 1, like [Danio rerio] 1.0E-133
blast AAT68046 KIAA0877-like [Danio rerio] ref|NP_001002868.1| dpy-19-like 1, like [Danio rerio] 1.0E-122
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498909.1 10 21-43,165-187,221-238,258-280,293-310,314-336,343-365,414-433,464-486,501-519
F22B7.10 10 21-43,165-187,221-238,258-280,293-310,314-336,343-365,414-433,464-486,501-519
GENEFINDER00000003841 1 607-629
SNAP00000003838 10 100-122,127-144,156-173,193-215,228-245,249-271,278-300,349-368,399-421,436-454
Sequence
Target Sequence 
>C. ELEGANS:188624_AT
gttgctgcactttttgcaaactccaagctgctggggggagatcgaatatcaaaaactata
cgagtttctgctcttgttggagtgatcgcaatattattttatagaggaattccaaatatc
cgtcaacagttgaatgtcaagggagaatacagtaatcctgatcaagaaatgttgtttgac
tggattcaacataacaccaaacaagacgctgtgttcgctggaacaatgccagtaatggca
aatgtgaagctcacaactcttaggccaattgtcaatcaccctcattatgaacatgttgga
atacgtgaacgaactctgaaagtgtattctatgtttagtaaaaaaccaatcgcggaagtt
cacaaaataatgaaagaaatgggtgtcaattattttgttttccaacttatgaattgttca
aatgatgagaggaggccagaatgtgtttatcgtggaatgtgggatgaagaagatccaaag
aatagtggtcgaacagctctttgtgatctttggatacttgctgcaaattcaaaagacaat
tcacgaatagctccatttaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGCTGCACTTTTTGCAAACTCCA 69 441 1459 Antisense
TGCAAACTCCAAGCTGCTGGGGGGA 597 571 1473 Antisense
TACGAGTTTCTGCTCTTGTTGGAGT 526 647 1517 Antisense
GAATTCCAAATATCCGTCAACAGTT 82 325 1565 Antisense
ACAAGACGCTGTGTTCGCTGGAACA 311 111 1659 Antisense
GTGAAGCTCACAACTCTTAGGCCAA 665 481 1702 Antisense
TTAGGCCAATTGTCAATCACCCTCA 45 681 1718 Antisense
GAGGAGGCCAGAATGTGTTTATCGT 659 403 1887 Antisense
GTGGTCGAACAGCTCTTTGTGATCT 336 489 1943 Antisense
TGTGATCTTTGGATACTTGCTGCAA 256 555 1960 Antisense
ACAATTCACGAATAGCTCCATTTAA 80 115 1994 Antisense
Sequence Source Affymetrix Proprietary Database