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[188620_s_at] SET domain

Description Y2H9A.1 /REP_DB=WormBase Gene ID /WP=CE27781 /TR=Q9XXS4 /GB=CAA16276.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=SET domain
Cluster 6grp_high1_10_10 > 77      4grp_high_8_8 > 45      6grp_total > 78      4grp_total > 59      6grp_cgc > 49      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 98
1 2 3 4 range
A 112.1
(-0.8)
P 		160.8
(3)
P 		157.2
(2.7)
P 		83.4
(-3)
A 		77
B 137.2
(3)
P 		123.2
(1.9)
A 		131.2
(2.5)
P 		63.1
(-3)
A 		74
C 125.1
(3)
P 		122
(-3)
P 		122.8
(-1.5)
P 		122.2
(-2.6)
P 		3
avr 124.8
(1.4) 		135.3
(2.8) 		137.1
(3) 		89.6
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 510
1 2 3 4 5 6 range
A 96.3
(-2.7)
A 		162
(-2)
P 		74.1
(-3)
P 		144.4
(-2.2)
P 		294.5
(-0.4)
P 		583.7
(3)
P 		510
B 157.2
(-2.8)
P 		161.8
(-2.7)
P 		141
(-3)
P 		191.8
(-2.3)
P 		249.7
(-1.5)
P 		580.1
(3)
P 		439
C 138.2
(-2.6)
P 		139.9
(-2.6)
P 		117.6
(-3)
P 		167.6
(-2.1)
P 		277.9
(-0.2)
P 		461.4
(3)
P 		344
avr 130.6
(-2.7) 		154.6
(-2.4) 		110.9
(-3) 		167.9
(-2.2) 		274
(-0.7) 		541.7
(3) 		431
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 40.8
(2.9)
P 		34.9
(1.7)
P 		41.1
(3)
P 		12.2
(-3)
A 		29
B 43.7
(3)
P 		30.4
(0.4)
A 		30.4
(0.4)
P 		13.5
(-3)
A 		30
C 41.4
(2.6)
P 		35.1
(0.9)
P 		42.8
(3)
P 		20.6
(-3)
P 		22
avr 42
(3) 		33.5
(1.1) 		38.1
(2.1) 		15.4
(-3) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 17.8
(-2.9)
A 		57.6
(-1)
P 		16.1
(-3)
P 		36.6
(-2)
P 		88.9
(0.5)
P 		142.1
(3)
P 		126
B 54.7
(-2.3)
P 		53.8
(-2.4)
P 		34.2
(-3)
P 		50.8
(-2.5)
P 		101.4
(-0.8)
P 		216.3
(3)
P 		182
C 51.2
(-2)
P 		42.7
(-2.4)
P 		29.9
(-3)
P 		33.7
(-2.8)
P 		89.9
(-0.1)
P 		154.5
(3)
P 		125
avr 41.2
(-2.4) 		51.4
(-2) 		26.7
(-3) 		40.4
(-2.4) 		93.4
(-0.2) 		171
(3) 		144
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188620_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13806
Sequence Type Exemplar sequence
Representative Public ID Y2H9A.1 NCBI
Target Description Y2H9A.1 /REP_DB=WormBase Gene ID /WP=CE27781 /TR=Q9XXS4 /GB=CAA16276.2 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=SET domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073932(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073932 NCBI Caenorhabditis elegans Maternal Effect Sterile family member (mes-4) (mes-4) mRNA, complete cds. 11/11 A 
SNAP00000011427 ENSEMBL cdna:SNAP chromosome:CEL140:V:13436033:13440055:-1 11/11 None
GENEFINDER00000011432 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13432424:13441241:-1 11/11 None
Y2H9A.1 ENSEMBL cdna:known chromosome:CEL140:V:13435648:13440136:-1 gene:Y2H9A.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13436032-13440055(-) 100.0 100.0
Public Domain and Genome References
Gene Title SET domain
Gene Symbol mes-4
Ensembl Y2H9A.1
Entrez Gene 179824 Entrez gene
SwissProt Q9NH52 EMBL-EBI
WormBase CE27781 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
20037 heme binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA16276 Hypothetical protein Y2H9A.1 [Caenorhabditis elegans] ref|NP_506333.1| Maternal Effect Sterile family member (mes-4) [Caenorhabditis elegans] gb|AAF62516.1| maternal-effect sterile 4 [Caenorhabditis elegans] sp|Q9NH52|MES4_CAEEL Probable histone methyltransferase mes-4 protein (Maternal-effect sterile 4 protein) 0.0
blast T26577 hypothetical protein Y2H9A.1 - Caenorhabditis elegans 0.0
blast CAA16277 Hypothetical protein Y2H9A.2 [Caenorhabditis elegans] ref|NP_506332.1| Serpentine Receptor, class D (delta) family member (srd-17) [Caenorhabditis elegans] 0.0
blast CAE62592 Hypothetical protein CBG06706 [Caenorhabditis briggsae] 1.0E-148
Protein Domains
Database ID Description E-Value
Pfam IPR001214 EMBL-EBI Nuclear protein SET 4.4E-38
Pfam IPR001214 EMBL-EBI Nuclear protein SET 1.5E-10
Pfam IPR001214 EMBL-EBI Nuclear protein SET 5.4E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000011432 7 1051-1073,1085-1107,1134-1156,1169-1191,1229-1251,1280-1302,1312-1334
Sequence
Target Sequence 
>C. ELEGANS:188620_S_AT
gaaatttctccttcgaaaccatccacaagctccgcgtcgagcacttccttcgtccaacaa
gcatcatggccaatttcccagaataagaaaaatttaaagaagaattccaatcaacctgta
gccgacaccggatcaacattgagtacatctacagagttgaatttccacgaaaaaccgcaa
gaactcttgagccctgtttcatcgcgatcgcgcgcagcttcatcttcaacaccacgggca
caaaaatcaaagagccgtcgagatgatgtggaaagtgaagcgccacctgtgaaacgagct
acgccatcattgcaaacaattcaagaaaccggaaaagcgatagagtttcctgcaaccaag
tcggctatcacaaaagcacgagcgttgtcgacacaatcagttccatcacctcatactgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAATTTCTCCTTCGAAACCATCCA 669 361 2218 Antisense
TTCCTTCGTCCAACAAGCATCATGG 429 699 2262 Antisense
AGCATCATGGCCAATTTCCCAGAAT 5 79 2277 Antisense
GAATTCCAATCAACCTGTAGCCGAC 559 323 2319 Antisense
AGCCGACACCGGATCAACATTGAGT 27 83 2337 Antisense
GCAAGAACTCTTGAGCCCTGTTTCA 17 321 2394 Antisense
GAAGCGCCACCTGTGAAACGAGCTA 490 343 2494 Antisense
AAACGAGCTACGCCATCATTGCAAA 56 127 2509 Antisense
TTCCTGCAACCAAGTCGGCTATCAC 457 699 2564 Antisense
GAGCGTTGTCGACACAATCAGTTCC 359 387 2597 Antisense
ATCAGTTCCATCACCTCATACTGTG 700 27 2613 Antisense
Sequence Source Affymetrix Proprietary Database