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[188611_s_at]

Description F21H12.5 /REP_DB=WormBase Gene ID /WP=CE01916 /GEN=fbf-2 /TR=SW:Q09312 /GB=AAC46716.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 86      4grp_total > 48      6grp_cgc > 68      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 33.6
(-2.3)
A 		26.7
(-3)
A 		88.3
(3)
P 		46.3
(-1.1)
A 		62
B 32.6
(-2.3)
A 		50.5
(-1)
A 		105.5
(3)
P 		22.5
(-3)
A 		83
C 43
(-3)
A 		63
(-0.1)
P 		85
(3)
P 		61.8
(-0.3)
A 		42
avr 36.4
(-3) 		46.7
(-1.9) 		92.9
(3) 		43.5
(-2.2) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 178
1 2 3 4 5 6 range
A 8.5
(-3)
A 		94
(0.1)
P 		75.1
(-0.6)
P 		115.4
(0.8)
P 		122.8
(1.1)
P 		175.3
(3)
P 		167
B 14.3
(-3)
A 		70.9
(-1)
P 		74.9
(-0.9)
P 		76.2
(-0.8)
M 		121.3
(0.7)
P 		186.8
(3)
P 		173
C 42.9
(-1.7)
A 		8.4
(-3)
A 		94.7
(0.1)
P 		83.2
(-0.3)
P 		143.9
(1.9)
P 		173.1
(3)
P 		165
avr 21.9
(-3) 		57.8
(-1.6) 		81.6
(-0.7) 		91.6
(-0.3) 		129.3
(1.1) 		178.4
(3) 		157
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 12.2
(-0.8)
A 		5.8
(-3)
A 		23.1
(3)
P 		6.8
(-2.7)
A 		17
B 10.4
(-1.3)
A 		12.5
(-0.7)
A 		24.5
(3)
P 		4.8
(-3)
A 		20
C 14.2
(-1.8)
A 		18.1
(-0.6)
P 		29.6
(3)
P 		10.4
(-3)
A 		19
avr 12.3
(-1.4) 		12.1
(-1.4) 		25.7
(3) 		7.3
(-3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 1.6
(-3)
A 		33.4
(1.6)
P 		16.3
(-0.9)
P 		29.3
(1)
P 		37.1
(2.2)
P 		42.7
(3)
P 		41
B 5
(-3)
A 		23.6
(-1.3)
P 		18.2
(-1.8)
P 		20.2
(-1.6)
M 		49.3
(1.1)
P 		69.7
(3)
P 		65
C 15.9
(-1.6)
A 		2.6
(-3)
A 		24.1
(-0.7)
P 		16.7
(-1.5)
P 		46.6
(1.8)
P 		57.9
(3)
P 		55
avr 7.5
(-3) 		19.9
(-1.5) 		19.5
(-1.5) 		22.1
(-1.2) 		44.3
(1.5) 		56.8
(3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188611_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5281
Sequence Type Exemplar sequence
Representative Public ID F21H12.5 NCBI
Target Description F21H12.5 /REP_DB=WormBase Gene ID /WP=CE01916 /GEN=fbf-2 /TR=SW:Q09312 /GB=AAC46716.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062819(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062819 NCBI Caenorhabditis elegans Fem-3 mRNA Binding Factor family member (fbf-2) (fbf-2) mRNA, complete cds. 11/11 A 
SNAP00000015298 ENSEMBL cdna:SNAP chromosome:CEL140:II:6089096:6091326:-1 11/11 None
GENEFINDER00000015304 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6089096:6091326:-1 11/11 None
F21H12.5 ENSEMBL cdna:known chromosome:CEL140:II:6088831:6091434:-1 gene:F21H12.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6089092-6091323(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol fbf-2
Ensembl F21H12.5
Entrez Gene 174017 Entrez gene
SwissProt Q09312 EMBL-EBI
WormBase CE01916 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3723 RNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495220 Fem-3 mRNA Binding Factor family member (fbf-2) [Caenorhabditis elegans] gb|AAC46716.1| Fem-3 mrna binding factor protein 2 [Caenorhabditis elegans] sp|Q09312|FBF2_CAEEL Fem-3 mRNA binding factor 0.0
blast AAF39879 Fem-3 mrna binding factor protein 1 [Caenorhabditis elegans] ref|NP_495216.1| Fem-3 mRNA Binding Factor family member (fbf-1) [Caenorhabditis elegans] sp|Q9N5M6|FBF1_CAEEL Fem-3 mRNA binding factor 0.0
blast CAE59353 Hypothetical protein CBG02702 [Caenorhabditis briggsae] 1.0E-112
Protein Domains
Database ID Description E-Value
Pfam IPR001313 EMBL-EBI Pumilio/Puf RNA-binding 3.3E-6
Sequence
Target Sequence 
>C. ELEGANS:188611_S_AT
aaaagtttgcttctcatgttgttgagaaagcattcctacatgcaccattggaacttcttg
ccgaaatgatggacgagattttcgacggatacattccacatccggacacgggaaaagatg
ccctcgatatcatgatgttccatcaattcggaaactacgtcgttcaatgcatgctgacta
tttgctgtgatgctgtgtctggaagacggcaaacgaaagaaggaggctatgaccatgcaa
tctcattccaggattggctgaaaaagttgcactcgagagttacgaaagaaaggcatcgct
tgtcaaggttctcatccggcaaaaagatgatcgagacgcttgccaatctccgttcaactc
atccgatttatgaactccaatcttcgggacacgattcattcaagacggattacttcagta
ctgcttccgagcacgatggtccggagttggagaagaatgggatcgaggaaggaagcctga
tgctagagccacggagcaataagtcttcagtctctgtcaaatt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAGTTTGCTTCTCATGTTGTTGA 67 133 1358 Antisense
TGCACCATTGGAACTTCTTGCCGAA 30 579 1398 Antisense
GGACACGGGAAAAGATGCCCTCGAT 320 525 1461 Antisense
GAAGGAGGCTATGACCATGCAATCT 231 335 1576 Antisense
TGACCATGCAATCTCATTCCAGGAT 110 571 1587 Antisense
CCAATCTTCGGGACACGATTCATTC 49 277 1734 Antisense
GGGACACGATTCATTCAAGACGGAT 612 493 1743 Antisense
GCTTCCGAGCACGATGGTCCGGAGT 339 305 1780 Antisense
GAAGCCTGATGCTAGAGCCACGGAG 12 345 1829 Antisense
GAGCCACGGAGCAATAAGTCTTCAG 456 385 1843 Antisense
ATAAGTCTTCAGTCTCTGTCAAATT 485 25 1856 Antisense
Sequence Source Affymetrix Proprietary Database