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[188607_at] Nitrilase

Description ZK1058.6 /REP_DB=WormBase Gene ID /WP=CE01106 /TR=Q23384 /GB=CAA84681.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Nitrilase
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 23      4grp_high_8_8 > 06      6grp_highest_nonorm > 98      6grp_total > 67      4grp_total > 02      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12758
1 2 3 4 range
A 1686
(-2.8)
P
1337.2
(-3)
P
1376
(-3)
P
14032.7
(3)
P
12696
B 1811.7
(-2.7)
P
1350
(-3)
P
1274.6
(-3)
P
13183.5
(3)
P
11909
C 1674.7
(-2.9)
P
1408
(-3)
P
1448.4
(-3)
P
12866.8
(3)
P
11459
avr 1724.1
(-2.8)
1365.1
(-3)
1366.3
(-3)
13361
(3)
11996

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1608
1 2 3 4 5 6 range
A 915.4
(-1.5)
P
524.9
(-3)
P
930.7
(-1.4)
P
967.8
(-1.2)
P
551.5
(-2.9)
P
2037.6
(3)
P
1513
B 1053.5
(-0.8)
P
462.3
(-3)
P
995.4
(-1)
P
835.9
(-1.6)
P
620.2
(-2.4)
P
2070.6
(3)
P
1608
C 925.5
(-1.4)
P
500.2
(-3)
P
1002.6
(-1.1)
P
957.9
(-1.2)
P
532
(-2.9)
P
2060.1
(3)
P
1560
avr 964.8
(-1.2)
495.8
(-3)
976.2
(-1.2)
920.5
(-1.4)
567.9
(-2.7)
2056.1
(3)
1560
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2519
1 2 3 4 range
A 613.9
(-1.9)
P
290.3
(-3)
P
359.7
(-2.8)
P
2054.1
(3)
P
1764
B 577.7
(-2.3)
P
333.4
(-2.9)
P
295.4
(-3)
P
2809.4
(3)
P
2514
C 554.4
(-2.5)
P
404.8
(-3)
P
504.8
(-2.7)
P
2169.5
(3)
P
1765
avr 582
(-2.3)
342.8
(-3)
386.6
(-2.9)
2344.3
(3)
2002

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 620
1 2 3 4 5 6 range
A 168.7
(-3)
P
186.7
(-2.6)
P
202.7
(-2.3)
P
245.4
(-1.6)
P
166.4
(-3)
P
496.1
(3)
P
330
B 366.6
(-0.9)
P
153.9
(-3)
P
241.7
(-2.1)
P
221.4
(-2.3)
P
252
(-2)
P
772.1
(3)
P
618
C 343
(-0.9)
P
152.6
(-3)
P
255.2
(-1.9)
P
192.6
(-2.6)
P
172.1
(-2.8)
P
689.6
(3)
P
537
avr 292.8
(-1.4)
164.4
(-3)
233.2
(-2.2)
219.8
(-2.3)
196.8
(-2.6)
652.6
(3)
488
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188607_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7353
Sequence Type Exemplar sequence
Representative Public ID ZK1058.6 NCBI
Target Description ZK1058.6 /REP_DB=WormBase Gene ID /WP=CE01106 /TR=Q23384 /GB=CAA84681.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Nitrilase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065390(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_065390 NCBI Caenorhabditis elegans ZK1058.6 (Nitrilase) mRNA, complete cds. 11/11 None
SNAP00000000034 ENSEMBL cdna:SNAP chromosome:CEL140:III:3922427:3923762:1 11/11 None
GENEFINDER00000000041 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:3922427:3923762:1 11/11 None
ZK1058.6 ENSEMBL cdna:known chromosome:CEL140:III:3922427:3923762:1 gene:ZK1058.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3922426-3923762(+) 100.0 100.0
Public Domain and Genome References
Gene Title Nitrilase
Ensembl ZK1058.6
Entrez Gene 191515 Entrez gene
SwissProt Q23384 EMBL-EBI
WormBase CE01106 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:252678_S_AT nitrilase 2 (NIT2) at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6807 nitrogen compound metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84681 Hypothetical protein ZK1058.6 [Caenorhabditis elegans] ref|NP_497791.1| ZK1058.6 [Caenorhabditis elegans] 1.0E-176
blast CAE60077 Hypothetical protein CBG03597 [Caenorhabditis briggsae] 1.0E-140
blast ZP_00665361 Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Syntrophobacter fumaroxidans MPOB] gb|EAO22040.1| Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase [Syntrophobacter fumaroxidans MPOB] 9.0E-93
Protein Domains
Database ID Description E-Value
Pfam IPR003010 EMBL-EBI Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase 2.2E-76
Sequence
Target Sequence
>C. ELEGANS:188607_AT
gaagcttcaaccttatactgctctgtattcttttactcaccctccggttaccttggaaag
caccgaaaactcttgccaactgctctagaaagatgtgtttggggacaaggtgatggttca
acaatgcctgtgtttagtacaagtgttggaaaaattggaagtgccatttgctgggagaat
tatatgccattatatcgaatgacgctttacagtaaagaaatccaaatctatctagctcca
actgtcgacgatagagatgtctggctctctaccatgaggactattgcattagaaggaaga
tgcttcgtagtatctgcatgtcaattcctcaaatcctccgactatcccttggatcaccca
cttcgaaaagaacacggtgaagataaagtcttgattcgcggtggatcatgcgcagttgat
ccacttggaactgtgctcgtggagccagatttcacaaaagaaacgattcgctacacggaa
ttcgatctttctgatttagctcttggaaaaatggatttagacgtcgtcggacactacagt
aggc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GAAGCTTCAACCTTATACTGCTCTG 378 341 328 Antisense
TACTGCTCTGTATTCTTTTACTCAC 207 647 343 Antisense
AAACTCTTGCCAACTGCTCTAGAAA 238 127 394 Antisense
ATCTAGCTCCAACTGTCGACGATAG 67 33 557 Antisense
TCTGGCTCTCTACCATGAGGACTAT 600 607 587 Antisense
GCTTCGTAGTATCTGCATGTCAATT 688 305 629 Antisense
ATGTCAATTCCTCAAATCCTCCGAC 708 47 645 Antisense
GATTCGCGGTGGATCATGCGCAGTT 371 433 720 Antisense
TGATCCACTTGGAACTGTGCTCGTG 40 567 744 Antisense
GCTACACGGAATTCGATCTTTCTGA 163 301 797 Antisense
GACGTCGTCGGACACTACAGTAGGC 162 313 847 Antisense
Sequence Source Affymetrix Proprietary Database