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[188604_at] histone H3

Description E03A3.3 /REP_DB=WormBase Gene ID /WP=CE00942 /TR=Q27489 /GB=CAA86228.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=histone H3
Cluster 6grp_total > 80      4grp_total > 17      6grp_cgc > 95      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6
1 2 3 4 range
A 7.5
(-3)
A 		8.3
(0.7)
A 		8.8
(3)
A 		8.1
(-0.2)
A 		1
B 5.4
(-3)
A 		6.1
(-2.2)
A 		7.7
(-0.5)
A 		10.9
(3)
A 		6
C 7.2
(3)
A 		7.1
(2.7)
A 		4.9
(-3)
A 		5.7
(-0.9)
A 		2
avr 6.7
(-3) 		7.2
(-1) 		7.1
(-1.4) 		8.2
(3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 7.4
(-0.6)
A 		5.6
(-2)
A 		6.7
(-1.1)
A 		4.2
(-3)
A 		9.3
(0.8)
A 		12.2
(3)
A 		8
B 3.8
(-2.6)
A 		11.1
(3)
A 		6.3
(-0.7)
A 		3.3
(-3)
A 		6.4
(-0.6)
A 		6.8
(-0.3)
A 		8
C 4.5
(-3)
A 		11.3
(-2)
A 		6.3
(-2.7)
A 		44.2
(3)
A 		6.1
(-2.8)
A 		6.8
(-2.7)
A 		40
avr 5.2
(-3) 		9.3
(-1) 		6.4
(-2.4) 		17.2
(3) 		7.3
(-2) 		8.6
(-1.3) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2
1 2 3 4 range
A 2.7
(3)
A 		1.8
(-0.6)
A 		2.3
(1.4)
A 		1.2
(-3)
A 		2
B 1.7
(-1.5)
A 		1.5
(-3)
A 		1.8
(-0.7)
A 		2.3
(3)
A 		1
C 2.4
(3)
A 		2
(1.3)
A 		1.7
(-0)
A 		1
(-3)
A 		1
avr 2.3
(3) 		1.8
(-0.7) 		1.9
(0) 		1.5
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.4
(-2.1)
A 		2
(-0.2)
A 		1.5
(-1.7)
A 		1.1
(-3)
A 		2.8
(2.4)
A 		3
(3)
A 		2
B 1.3
(-2.1)
A 		3.7
(3)
A 		1.5
(-1.7)
A 		0.9
(-3)
A 		2.6
(0.6)
A 		2.5
(0.4)
A 		3
C 1.7
(-2.9)
A 		3.4
(-1.5)
A 		1.6
(-3)
A 		8.9
(3)
A 		2
(-2.7)
A 		2.3
(-2.4)
A 		7
avr 1.5
(-3) 		3
(1.3) 		1.5
(-3) 		3.6
(3) 		2.5
(-0.1) 		2.6
(0.1) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188604_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7685
Sequence Type Exemplar sequence
Representative Public ID E03A3.3 NCBI
Target Description E03A3.3 /REP_DB=WormBase Gene ID /WP=CE00942 /TR=Q27489 /GB=CAA86228.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=histone H3
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065410(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065410 NCBI Caenorhabditis elegans HIStone family member (his-69) (his-69) mRNA, complete cds. 11/11 None
SNAP00000016640 ENSEMBL cdna:SNAP chromosome:CEL140:III:4058330:4058713:-1 11/11 None
GENEFINDER00000016651 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4058330:4058713:-1 11/11 None
E03A3.3 ENSEMBL cdna:known chromosome:CEL140:III:4058330:4058713:-1 gene:E03A3.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4058329-4058713(-) 100.0 100.0
Public Domain and Genome References
Gene Title histone H3
Gene Symbol his-69
Ensembl E03A3.3
Entrez Gene 184005 Entrez gene
SwissProt Q27489 EMBL-EBI
WormBase CE00942 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6334 nucleosome assembly inferred from electronic annotation QuickGO AmiGO
7001 chromosome organization and biogenesis (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
786 nucleosome inferred from electronic annotation QuickGO AmiGO
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86228 Hypothetical protein E03A3.3 [Caenorhabditis elegans] ref|NP_497811.1| HIStone family member (his-69) [Caenorhabditis elegans] 7.0E-66
blast CAA98963 Hypothetical protein W05B10.1 [Caenorhabditis elegans] ref|NP_506164.1| W05B10.1 [Caenorhabditis elegans] 9.0E-56
blast BAD90809.1 histone 3 [Conocephalum conicum] 2.0E-55
Protein Domains
Database ID Description E-Value
Pfam IPR007125 EMBL-EBI Histone core 3.0E-26
Sequence
Target Sequence 
>C. ELEGANS:188604_AT
atgtgtcccggaggaaaagcaccaagaaagcaattggcaacgaaagctgctcgaaagaac
gcaatcgtcgtcggagccgtgaagaagccgcatcgtttccgtcctggaactgtcgctctt
cgcgagatcagacgctatcaaaagtccactgatcttcttctccgcaagctgccattccaa
cgtcttgttcgtgaaatcgctcaagacgtcaaacaggatcttcgcttccaatcggctgca
attcaagctcttcaagaggcctccgagtacttccttgtcggactc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTGTCCCGGAGGAAAAGCACCAA 295 49 13 Antisense
GCAATTGGCAACGAAAGCTGCTCGA 232 323 42 Antisense
CGAAAGAACGCAATCGTCGTCGGAG 205 253 64 Antisense
GTCGGAGCCGTGAAGAAGCCGCATC 80 473 82 Antisense
AACTGTCGCTCTTCGCGAGATCAGA 339 143 120 Antisense
CAAAAGTCCACTGATCTTCTTCTCC 245 191 151 Antisense
GAAATCGCTCAAGACGTCAAACAGG 34 363 205 Antisense
CGTCAAACAGGATCTTCGCTTCCAA 236 243 219 Antisense
CGCTTCCAATCGGCTGCAATTCAAG 239 255 235 Antisense
CAAGCTCTTCAAGAGGCCTCCGAGT 64 187 256 Antisense
CTCCGAGTACTTCCTTGTCGGACTC 152 229 273 Antisense
Sequence Source Affymetrix Proprietary Database