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[188600_at]

Description K10G6.1 /REP_DB=WormBase Gene ID /WP=CE12098 /GEN=lin-31 /TR=SW:P34683 /GB=AAB66096.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 22      4grp_total > 52      6grp_cgc > 61      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 103
1 2 3 4 range
A 131
(2.3)
A 		79.9
(-3)
A 		137.8
(3)
A 		96.8
(-1.2)
A 		58
B 154.4
(0.7)
A 		182.5
(3)
A 		148.1
(0.2)
A 		108.3
(-3)
A 		74
C 144.1
(-0.3)
A 		169.1
(3)
A 		124.2
(-3)
A 		141.2
(-0.7)
A 		45
avr 143.2
(2.9) 		143.8
(3) 		136.7
(1.5) 		115.4
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 121
1 2 3 4 5 6 range
A 138.2
(2.1)
A 		150.8
(3)
A 		130.4
(1.5)
A 		89.5
(-1.5)
A 		137.5
(2)
A 		69.2
(-3)
A 		82
B 167.7
(2.5)
A 		176.1
(3)
A 		158.9
(2)
A 		151.7
(1.6)
A 		128.5
(0.2)
A 		74.8
(-3)
A 		101
C 113.1
(0)
A 		170
(3)
A 		128.4
(0.8)
A 		135.8
(1.2)
A 		82
(-1.6)
A 		54.8
(-3)
A 		115
avr 139.7
(1.4) 		165.6
(3) 		139.2
(1.4) 		125.7
(0.6) 		116
(0) 		66.3
(-3) 		99
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 35
1 2 3 4 range
A 47.7
(3)
A 		17.3
(-2.4)
A 		36
(0.9)
A 		14.2
(-3)
A 		34
B 49.2
(3)
A 		45.1
(2.1)
A 		34.3
(-0.4)
A 		23.1
(-3)
A 		26
C 47.7
(2.8)
A 		48.6
(3)
A 		43.3
(1.7)
A 		23.8
(-3)
A 		25
avr 48.2
(3) 		37
(0.6) 		37.9
(0.8) 		20.4
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 25.5
(-1.6)
A 		53.6
(3)
A 		28.4
(-1.1)
A 		22.7
(-2.1)
A 		41.5
(1)
A 		16.9
(-3)
A 		37
B 58.3
(2.9)
A 		58.6
(3)
A 		38.6
(-0.9)
A 		40.2
(-0.6)
A 		52.2
(1.7)
A 		27.9
(-3)
A 		31
C 41.9
(1.2)
A 		51.9
(3)
A 		32.7
(-0.4)
A 		27.3
(-1.4)
A 		26.5
(-1.5)
A 		18.3
(-3)
A 		34
avr 41.9
(0.7) 		54.7
(3) 		33.2
(-0.8) 		30.1
(-1.4) 		40.1
(0.4) 		21
(-3) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188600_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5038
Sequence Type Exemplar sequence
Representative Public ID K10G6.1 NCBI
Target Description K10G6.1 /REP_DB=WormBase Gene ID /WP=CE12098 /GEN=lin-31 /TR=SW:P34683 /GB=AAB66096.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062303(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062303 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-31) (lin-31) mRNA, complete cds. 11/11 None
K10G6.1 ENSEMBL cdna:known chromosome:CEL140:II:3982567:3986958:1 gene:K10G6.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000011083 5/11 Cross Hyb Matching Probes None
GENEFINDER00000011087 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3982646-3986460(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-31
Ensembl K10G6.1
Entrez Gene 173740 Entrez gene
SwissProt P34683 EMBL-EBI
WormBase CE12098 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1631857_AT forkhead domain 96Ca dm
DROSGENOME1:143531_AT forkhead domain 96Ca dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_494704 abnormal cell LINeage family member (lin-31) [Caenorhabditis elegans] gb|AAB66096.1| Abnormal cell lineage protein 31 [Caenorhabditis elegans] sp|P34683|LIN31_CAEEL Abnormal cell lineage protein 31 (Protein lin-31) gb|AAA28104.1| lin-31 protein 8.0E-82
blast CAE71507 Hypothetical protein CBG18439 [Caenorhabditis briggsae] 9.0E-67
blast CAD31848 FoxB2 protein [Xenopus laevis] 2.0E-45
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 9.0005400363583E-42
Pfam IPR001766 EMBL-EBI Fork head transcription factor 3.2E-69
Sequence
Target Sequence 
>C. ELEGANS:188600_AT
taaaatgctcccgctcacggagatctacaaatatattatggatcggtttccgttttatag
gaaaaacacgcagaggtggcaaaactccctgcgacacaacttgtcattcaacgactgctt
catcaagatcccaagaagagccgatcgtcctgggaagggctcctactgggcggtgcaccc
aaatgcctccggaatgttcgagaacggcagctgcttacggcgtcggaaacgatttcgggc
tcgcggcggccaagacgacgacgacgacgacttccaccacccggctccatcgaaaatctc
tcgcaaaaatcctcttccactgctcccggagcccccaataaccccaccactcctctcctc
ccttttcccaaatcttccaccatcactcccaaatttctgcctgtttcctccaggcatgga
tcctacaaaatcgttgcttctgaaccctctttccctacttttaatgccccatttcttgaa
aaattcttcgaattttgagagctcgactccacactctgaaacatcggagatttctgggtc
gggatcttcatcgtcgaagactccaactcctgaagctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAAATGCTCCCGCTCACGGAGATC 375 639 105 Antisense
CAACGACTGCTTCATCAAGATCCCA 569 187 213 Antisense
CAAGAAGAGCCGATCGTCCTGGGAA 574 185 236 Antisense
ACCCAAATGCCTCCGGAATGTTCGA 482 89 281 Antisense
CAGCTGCTTACGGCGTCGGAAACGA 528 201 312 Antisense
AAAATCTCTCGCAAAAATCCTCTTC 666 133 397 Antisense
TCTGCCTGTTTCCTCCAGGCATGGA 339 609 500 Antisense
ACAAAATCGTTGCTTCTGAACCCTC 383 111 529 Antisense
TCGACTCCACACTCTGAAACATCGG 317 601 607 Antisense
ATTTCTGGGTCGGGATCTTCATCGT 180 17 634 Antisense
CGAAGACTCCAACTCCTGAAGCTGG 281 253 659 Antisense
Sequence Source Affymetrix Proprietary Database