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[188599_s_at]

Description F45E12.3 /REP_DB=WormBase Gene ID /WP=CE24979 /GEN=cul-4 /TR=SW:Q17392 /GB=AAA68791.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 66      6grp_high1_10_10 > 66      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_highest_nonorm > 81      6grp_total > 57      4grp_total > 55      6grp_highest_sub > Da      6grp_cgc > 37      6grp_high1_sub > Da      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 250
1 2 3 4 range
A 328.4
(3)
P 		203.1
(-1.8)
P 		194.6
(-2.1)
P 		170.8
(-3)
P 		158
B 304
(3)
P 		162.4
(-2.2)
P 		236
(0.5)
P 		141.8
(-3)
P 		162
C 392
(3)
P 		164.6
(-3)
P 		222.6
(-1.5)
P 		178.9
(-2.6)
P 		227
avr 341.5
(3) 		176.7
(-2.6) 		217.7
(-1.2) 		163.8
(-3) 		178

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 103
1 2 3 4 5 6 range
A 241.7
(-3)
P 		283.6
(0.6)
P 		286.7
(0.9)
P 		283.4
(0.6)
P 		256.2
(-1.8)
P 		311.8
(3)
P 		70
B 252.9
(-3)
P 		265
(-2.2)
P 		265.6
(-2.2)
P 		276.3
(-1.5)
P 		272.5
(-1.7)
P 		344.8
(3)
P 		92
C 344.2
(3)
P 		261.6
(-2.2)
P 		287.7
(-0.6)
P 		278.6
(-1.2)
P 		249.7
(-3)
P 		315.2
(1.2)
P 		95
avr 279.6
(-1.1) 		270.1
(-2) 		280
(-1.1) 		279.4
(-1.1) 		259.5
(-3) 		323.9
(3) 		64
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 105
1 2 3 4 range
A 119.6
(3)
P 		44.1
(-1.8)
P 		50.9
(-1.4)
P 		25
(-3)
P 		95
B 97
(3)
P 		40.1
(-2.1)
P 		54.7
(-0.8)
P 		30.2
(-3)
P 		67
C 129.7
(3)
P 		47.3
(-2)
P 		77.6
(-0.1)
P 		30.2
(-3)
P 		100
avr 115.4
(3) 		43.8
(-1.9) 		61.1
(-0.7) 		28.5
(-3) 		87

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 44.5
(-3)
P 		100.8
(3)
P 		62.4
(-1.1)
P 		71.9
(-0.1)
P 		77.3
(0.5)
P 		75.9
(0.3)
P 		56
B 88
(-0.8)
P 		88.2
(-0.8)
P 		64.5
(-3)
P 		73.2
(-2.2)
P 		110.7
(1.3)
P 		128.6
(3)
P 		64
C 127.6
(3)
P 		79.8
(-1)
P 		73.2
(-1.6)
P 		56
(-3)
P 		80.8
(-0.9)
P 		105.5
(1.1)
P 		72
avr 86.7
(0.3) 		89.6
(0.8) 		66.7
(-3) 		67
(-3) 		89.6
(0.8) 		103.3
(3) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188599_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5795
Sequence Type Exemplar sequence
Representative Public ID F45E12.3 NCBI
Target Description F45E12.3 /REP_DB=WormBase Gene ID /WP=CE24979 /GEN=cul-4 /TR=SW:Q17392 /GB=AAA68791.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063124(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063124 NCBI Caenorhabditis elegans CULlin family member (cul-4) (cul-4) mRNA, complete cds. 11/11 None
SNAP00000037596 ENSEMBL cdna:SNAP chromosome:CEL140:II:7302042:7305461:1 11/11 None
GENEFINDER00000037604 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7302042:7305461:1 11/11 None
F45E12.3 ENSEMBL cdna:known chromosome:CEL140:II:7302032:7305693:1 gene:F45E12.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7302037-7305458(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol cul-4
Ensembl F45E12.3
Entrez Gene 174198 Entrez gene
SwissProt Q17392 EMBL-EBI
WormBase CE36545 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7049 cell cycle inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68791 Cullin protein 4 [Caenorhabditis elegans] ref|NP_495525.2| CULlin family member (cul-4) [Caenorhabditis elegans] sp|Q17392|CUL4_CAEEL Cullin-4 0.0
blast AAC47123 CUL-4 0.0
blast T16367 hypothetical protein F45E12.3 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.SCF ubiquitin ligase complex WHB domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; SCF ubiquitin ligase complex WHB domain 8.00000015633185E-24
Pfam IPR001373 EMBL-EBI Cullin 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188599_S_AT
taaatggcatcacggactggcttctgcggttatttctgcttcattcaggcctggatgtaa
gaaagaattaattgcaacaatgtatcaaactgtcattcttcttcttttcaacaaatgtga
gacatggactgtcgctgaaattgttgagcacactaagattctggaaattgaagtcgtcaa
aaatgtgcttgcacttcttggtggacgtgataagcctaaggttttgcagagagtcgaagg
aggtggatccgaaaagaaggaaggtactgtggaaaatttgaaaaacgagaaatttgttgt
aaattctaaattcacggagaagcgctgccgagttaggattgctcaggtcaatataaagac
agctgtggaagagacgaaggaagtgaaagaagaagtgaattctgatcgacaatacaaaat
tgacgctgctgttgtaagaattatgaaggctcgaaagcagctaaaccatcaaactctaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAATGGCATCACGGACTGGCTTCT 357 641 1902 Antisense
ATTTCTGCTTCATTCAGGCCTGGAT 233 15 1933 Antisense
GTATCAAACTGTCATTCTTCTTCTT 60 455 1983 Antisense
GACATGGACTGTCGCTGAAATTGTT 652 369 2022 Antisense
TGTTGAGCACACTAAGATTCTGGAA 317 563 2043 Antisense
TGTGCTTGCACTTCTTGGTGGACGT 93 557 2085 Antisense
GGTGGACGTGATAAGCCTAAGGTTT 300 497 2101 Antisense
AATTCACGGAGAAGCGCTGCCGAGT 152 177 2210 Antisense
GCGCTGCCGAGTTAGGATTGCTCAG 168 285 2223 Antisense
AAATTGACGCTGCTGTTGTAAGAAT 468 113 2318 Antisense
AAGCAGCTAAACCATCAAACTCTAA 413 149 2356 Antisense
Sequence Source Affymetrix Proprietary Database