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[188596_at]

Description H20J04.4 /REP_DB=WormBase Gene ID /WP=CE23814 /TR=Q9N5M1 /GB=AAF39890.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=helicase
Cluster 6grp_high1_10_10 > 66      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_total > 67      4grp_total > 14      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 189
1 2 3 4 range
A 306.8
(2.3)
P 		184.1
(-1.6)
P 		141.1
(-3)
P 		329.7
(3)
P 		189
B 243
(-1.3)
P 		227.6
(-2.6)
P 		222.9
(-3)
P 		292.5
(3)
P 		70
C 290.1
(3)
P 		207.5
(-3)
P 		226.8
(-1.6)
P 		288.3
(2.9)
P 		83
avr 280
(1.7) 		206.4
(-2.5) 		196.9
(-3) 		303.5
(3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 444
1 2 3 4 5 6 range
A 78.6
(-2.8)
P 		90
(-2.4)
P 		105.3
(-2)
P 		72.1
(-3)
P 		80.2
(-2.8)
P 		267
(3)
P 		195
B 116.9
(-2.2)
P 		85.8
(-2.7)
P 		136.7
(-1.9)
P 		138.8
(-1.9)
P 		64.3
(-3)
P 		467.2
(3)
P 		403
C 36.3
(-3)
A 		84.1
(-2.4)
P 		77.6
(-2.4)
P 		138.7
(-1.6)
P 		60.1
(-2.7)
P 		480.3
(3)
P 		444
avr 77.3
(-2.8) 		86.6
(-2.7) 		106.5
(-2.3) 		116.5
(-2.1) 		68.2
(-3) 		404.8
(3) 		337
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 75
1 2 3 4 range
A 111.7
(3)
P 		40
(-2.8)
P 		36.9
(-3)
P 		48.3
(-2.1)
P 		75
B 77.5
(3)
P 		56.2
(-1.9)
P 		51.6
(-3)
P 		62.3
(-0.5)
P 		26
C 96
(3)
P 		59.7
(-1.6)
P 		79
(0.8)
P 		48.6
(-3)
P 		47
avr 95.1
(3) 		52
(-3) 		55.8
(-2.5) 		53.1
(-2.8) 		43

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 161
1 2 3 4 5 6 range
A 14.5
(-3)
P 		32
(-0.9)
P 		22.9
(-2)
P 		18.3
(-2.5)
P 		24.2
(-1.8)
P 		65
(3)
P 		51
B 40.7
(-2.4)
P 		28.6
(-2.9)
P 		33.2
(-2.7)
P 		36.8
(-2.6)
P 		26.1
(-3)
P 		174.2
(3)
P 		148
C 13.5
(-3)
A 		25.7
(-2.5)
P 		19.7
(-2.7)
P 		27.9
(-2.4)
P 		19.5
(-2.8)
P 		160.8
(3)
P 		147
avr 22.9
(-3) 		28.8
(-2.7) 		25.3
(-2.9) 		27.7
(-2.7) 		23.3
(-3) 		133.3
(3) 		110
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188596_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4073
Sequence Type Exemplar sequence
Representative Public ID H20J04.4 NCBI
Target Description H20J04.4 /REP_DB=WormBase Gene ID /WP=CE23814 /TR=Q9N5M1 /GB=AAF39890.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062364
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000017488 ENSEMBL cdna:SNAP chromosome:CEL140:II:4329025:4350547:1 None
GENEFINDER00000017494 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4328677:4338418:1 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_062364 3/11 Cross Hyb Matching Probes None
H20J04.4 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4329023-4334511(+) 81.83 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF39888 Hypothetical protein H20J04.2 [Caenorhabditis elegans] ref|NP_494767.1| H20J04.2 [Caenorhabditis elegans] 0.0
blast CAE60124 Hypothetical protein CBG03667 [Caenorhabditis briggsae] 0.0
blast AAF39890 Hypothetical protein H20J04.4 [Caenorhabditis elegans] ref|NP_494765.2| H20J04.4 [Caenorhabditis elegans] 0.0
blast AAF39889 Hypothetical protein H20J04.3 [Caenorhabditis elegans] ref|NP_494766.1| H20J04.3 [Caenorhabditis elegans] 1.0E-180
Protein Domains
Database ID Description E-Value
Pfam IPR001965 EMBL-EBI Zn-finger-like, PHD finger 1.5E-10
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.8E-31
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.2E-75
Pfam IPR001487 EMBL-EBI Bromodomain 2.3E-30
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.8E-31
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.6E-61
Sequence
Target Sequence 
>C. ELEGANS:188596_AT
ggatctgcgctgagttttgttacacaatatgatgtggaacttctgcaggctgtggagcag
gttattgggaagaagctcgatgagttgaaggtcagcccaaaacatgtcaccaaatacgtc
acccaagtgcttgtggcgaagaaagaagccgagttgaagctggaaaatcagaaattcgga
gagaaaaaggagattaatcgaagaaaagagcttctgatgagtggaatggatgaagatgaa
gctgataggcatttggaggaaatgaggactcgaagaatgacaaattccaagagaaagctg
gagaaaattagtggacaattggataaaagagatcgatttcagaagaaattggcggcgaaa
aaaaaaaccgacaaaaaaatttcaaaaaagcacagtttcaagtttttccgtctcgtaaaa
attcttccaaaaaattcctgcaaatttatgttcggtaaatcggcaggtcgacaattcgcc
gatttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGATCTGCGCTGAGTTTTGTTACAC 175 513 1486 Antisense
TGATGTGGAACTTCTGCAGGCTGTG 192 567 1515 Antisense
GCAGGCTGTGGAGCAGGTTATTGGG 81 311 1530 Antisense
GCTCGATGAGTTGAAGGTCAGCCCA 629 297 1560 Antisense
GTCAGCCCAAAACATGTCACCAAAT 361 465 1576 Antisense
TCACCAAATACGTCACCCAAGTGCT 272 625 1592 Antisense
ACGTCACCCAAGTGCTTGTGGCGAA 159 93 1601 Antisense
GAAGCTGATAGGCATTTGGAGGAAA 272 343 1723 Antisense
GCACAGTTTCAAGTTTTTCCGTCTC 561 315 1875 Antisense
TTATGTTCGGTAAATCGGCAGGTCG 141 665 1931 Antisense
GCAGGTCGACAATTCGCCGATTTGC 619 311 1948 Antisense
Sequence Source Affymetrix Proprietary Database