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[188594_at] protease

Description T02B11.7 /REP_DB=WormBase Gene ID /WP=CE27395 /TR=O16977 /GB=AAB69905.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 10      4grp_highest_8_8 > 62      4grp_high_8_8 > 26      6grp_highest_nonorm > 90      6grp_total > 23      4grp_total > 37      6grp_cgc > 20      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 155
1 2 3 4 range
A 247.2
(1.8)
P 		246.4
(1.7)
P 		197.7
(-3)
P 		259.6
(3)
P 		62
B 220.8
(3)
P 		207.7
(2.2)
P 		199.2
(1.7)
P 		124.3
(-3)
P 		97
C 279.2
(3)
P 		201.5
(-0.1)
P 		127.5
(-3)
P 		275.2
(2.8)
P 		152
avr 249.1
(3) 		218.5
(0.5) 		174.8
(-3) 		219.7
(0.6) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 149
1 2 3 4 5 6 range
A 210.4
(3)
P 		186.6
(1.5)
P 		160.6
(-0.2)
P 		155.8
(-0.5)
P 		121
(-2.7)
P 		116
(-3)
P 		94
B 167.5
(3)
P 		132.1
(0.7)
P 		151.9
(2)
P 		145.6
(1.6)
P 		73.4
(-3)
P 		131.8
(0.7)
P 		94
C 208.4
(2.4)
P 		145.7
(-0.2)
P 		190.7
(1.7)
P 		222
(3)
P 		88.9
(-2.7)
P 		81
(-3)
P 		141
avr 195.4
(3) 		154.8
(0.6) 		167.7
(1.4) 		174.5
(1.8) 		94.4
(-3) 		109.6
(-2.1) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 90
(3)
P 		53.5
(-1.2)
P 		51.7
(-1.4)
P 		38
(-3)
P 		52
B 70.4
(3)
P 		51.3
(0.4)
P 		46.2
(-0.3)
P 		26.5
(-3)
P 		44
C 92.4
(3)
P 		57.9
(-1.3)
P 		44.4
(-3)
P 		46.4
(-2.8)
P 		48
avr 84.3
(3) 		54.2
(-0.8) 		47.4
(-1.7) 		37
(-3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 50
1 2 3 4 5 6 range
A 38.8
(-1.3)
P 		66.4
(3)
P 		35
(-1.9)
P 		39.5
(-1.2)
P 		36.5
(-1.7)
P 		28.2
(-3)
P 		38
B 58.3
(3)
P 		44
(-0)
P 		36.9
(-1.5)
P 		38.6
(-1.1)
P 		29.8
(-3)
P 		49.1
(1.1)
P 		29
C 77.2
(3)
P 		44.5
(-0.9)
P 		48.5
(-0.4)
P 		44.6
(-0.9)
P 		28.8
(-2.8)
P 		27.1
(-3)
P 		50
avr 58.1
(3) 		51.6
(1.5) 		40.1
(-1.1) 		40.9
(-0.9) 		31.7
(-3) 		34.8
(-2.3) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188594_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14953
Sequence Type Exemplar sequence
Representative Public ID T02B11.7 NCBI
Target Description T02B11.7 /REP_DB=WormBase Gene ID /WP=CE27395 /TR=O16977 /GB=AAB69905.2 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070950(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070950 NCBI Caenorhabditis elegans T02B11.7 (protease) mRNA, complete cds. 9/11 A 
SNAP00000007475 ENSEMBL cdna:SNAP chromosome:CEL140:V:894760:898962:-1 9/11 None
GENEFINDER00000007485 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:894760:898962:-1 10/11 None
T02B11.7 ENSEMBL cdna:known chromosome:CEL140:V:894760:898981:-1 gene:T02B11.7 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:894662-898962(-) 99.57 100.0
Public Domain and Genome References
Gene Title protease
Gene Symbol nas-32
Ensembl T02B11.7
Entrez Gene 178595 Entrez gene
SwissProt O16977 EMBL-EBI
WormBase CE36189 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB69905 Nematode astacin protease protein 32 [Caenorhabditis elegans] ref|NP_503351.2| T02B11.7 [Caenorhabditis elegans] sp|O16977|NAS32_CAEEL Zinc metalloproteinase nas-32 precursor (Nematode astacin 32) 0.0
blast CAE71052 Hypothetical protein CBG17894 [Caenorhabditis briggsae] 0.0
blast CAA98497 Hypothetical protein F58B4.1a [Caenorhabditis elegans] ref|NP_001023993.1| Nematode AStacin protease family member (nas-31) [Caenorhabditis elegans] 8.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 9.1E-70
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-8
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 3.1E-5
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 9.1E-70
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.6E-8
Sequence
Target Sequence 
>C. ELEGANS:188594_AT
aatcattggattcaggcgcctgtcggtaagcaaattcaatttcgaatcgattccacgtac
aacacccaatgtgtctacgggtgcacatttaatggagttgaaccgaagttgaaaagtgac
atgacaattacgcaggcgaggtactgttgcgacgagttcaacgctgagatcatgactgcc
gacttcggtgtcaatccgatgcccgtcttcagctttaatcggtactacaaaaccacgtac
acatggtcatatcgatacgtggactctaacgtcaccgcatgtgctgatacatccgacaaa
gccacgtgtctttctttgaaaagtgcaaaagagcagggttgctcaatttatgacactgct
cagctgaaggtgatgtgtgcggcgactatggatttgtgtgggaaggtggcgtcagatgat
gggacatatgctctacctactccacgaacggaatgtgcacccaacaaccgccacttgccg
ccgaattctcatgcgctgaaacttgcggtttctgtactaatccggtttgaaataaaatat
tttgtgttcgttactgttcttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCATTGGATTCAGGCGCCTGTCG 505 169 1489 Antisense
GGCGCCTGTCGGTAAGCAAATTCAA 528 541 1503 Antisense
ATTCCACGTACAACACCCAATGTGT 683 5 1538 Antisense
GCAGGCGAGGTACTGTTGCGACGAG 148 311 1620 Antisense
AGATCATGACTGCCGACTTCGGTGT 436 65 1655 Antisense
ACTTCGGTGTCAATCCGATGCCCGT 397 101 1670 Antisense
ATGCCCGTCTTCAGCTTTAATCGGT 441 39 1687 Antisense
TGATGGGACATATGCTCTACCTACT 503 567 1905 Antisense
GCCGAATTCTCATGCGCTGAAACTT 194 285 1968 Antisense
ACTTGCGGTTTCTGTACTAATCCGG 15 103 1989 Antisense
TATTTTGTGTTCGTTACTGTTCTTT 500 663 2026 Antisense
Sequence Source Affymetrix Proprietary Database