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[188591_at]

Description T05A12.4 /REP_DB=WormBase Gene ID /WP=CE26384 /TR=Q9GYK7 /GB=AAF98590.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=helicase
Cluster 6grp_highest_10_10 > 12      6grp_high1_10_10 > 20      4grp_highest_8_8 > 51      4grp_high_8_8 > 24      6grp_highest_nonorm > 70      6grp_total > 23      4grp_total > 39      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 272
1 2 3 4 range
A 383.1
(3)
P 		238.2
(-0.2)
P 		111.3
(-3)
P 		120.8
(-2.8)
P 		272
B 377.1
(3)
P 		125.1
(-3)
P 		147.1
(-2.5)
P 		201
(-1.2)
P 		252
C 339.4
(3)
P 		132.8
(-3)
P 		182.4
(-1.6)
P 		248.4
(0.4)
P 		207
avr 366.5
(3) 		165.4
(-2.5) 		146.9
(-3) 		190.1
(-1.8) 		220

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 318
1 2 3 4 5 6 range
A 394.5
(1.8)
P 		453.8
(3)
P 		244.6
(-1.2)
P 		332
(0.5)
P 		157.1
(-3)
P 		264
(-0.8)
P 		297
B 347
(3)
P 		290.4
(1.1)
P 		327.3
(2.3)
P 		312.4
(1.8)
P 		169.5
(-3)
P 		172.7
(-2.9)
P 		178
C 317
(0.7)
P 		329.8
(0.9)
P 		430.7
(3)
P 		386.2
(2.1)
P 		135.6
(-3)
P 		169.8
(-2.3)
P 		295
avr 352.8
(2.8) 		358
(3) 		334.2
(2.3) 		343.5
(2.6) 		154.1
(-3) 		202.2
(-1.6) 		204
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 122
1 2 3 4 range
A 139.5
(3)
P 		51.7
(-1.3)
P 		29.1
(-2.4)
P 		17.7
(-3)
P 		122
B 120.3
(3)
P 		30.9
(-3)
P 		34.1
(-2.8)
P 		42.8
(-2.2)
P 		89
C 112.4
(3)
P 		38.2
(-3)
P 		63.6
(-0.9)
P 		41.9
(-2.7)
P 		74
avr 124.1
(3) 		40.3
(-2.6) 		42.3
(-2.5) 		34.1
(-3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 72.7
(-1.7)
P 		161.4
(3)
P 		53.3
(-2.7)
P 		84.2
(-1.1)
P 		47.4
(-3)
P 		64.3
(-2.1)
P 		114
B 120.7
(3)
P 		96.6
(0.4)
P 		79.5
(-1.4)
P 		82.7
(-1)
P 		68.8
(-2.5)
P 		64.4
(-3)
P 		56
C 117.5
(3)
P 		100.6
(1.6)
P 		109.6
(2.4)
P 		77.7
(-0.2)
P 		43.9
(-3)
P 		56.8
(-1.9)
P 		74
avr 103.6
(1.6) 		119.5
(3) 		80.8
(-0.5) 		81.5
(-0.4) 		53.4
(-3) 		61.8
(-2.2) 		66
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188591_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8956
Sequence Type Exemplar sequence
Representative Public ID T05A12.4 NCBI
Target Description T05A12.4 /REP_DB=WormBase Gene ID /WP=CE26384 /TR=Q9GYK7 /GB=AAF98590.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028188(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028188 NCBI Caenorhabditis elegans T05A12.4a (helicase) mRNA, complete cds. 11/11 None
SNAP00000024004 ENSEMBL cdna:SNAP chromosome:CEL140:IV:6890855:6897512:1 11/11 None
GENEFINDER00000024023 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:6890900:6897512:1 11/11 None
T05A12.4a ENSEMBL cdna:known chromosome:CEL140:IV:6888965:6897761:1 gene:T05A12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177453 2 1 NM_001028188 173010_at (9), 188591_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:6887890-6897518(+) 99.42 100.0
Public Domain and Genome References
Ensembl T05A12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV28333 Hypothetical protein T05A12.4a [Caenorhabditis elegans] ref|NP_001023359.1| T05A12.4a [Caenorhabditis elegans] 0.0
blast AAV28334 Hypothetical protein T05A12.4b [Caenorhabditis elegans] ref|NP_001023360.1| T05A12.4b [Caenorhabditis elegans] 0.0
blast T32703 hypothetical protein T22D1.7 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000330 EMBL-EBI SNF2 related domain 4.4E-24
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 7.1E-10
Pfam IPR001841 EMBL-EBI Zn-finger, RING 7.7E-6
Pfam IPR000330 EMBL-EBI SNF2 related domain 2.4E-5
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 7.1E-10
Pfam IPR001841 EMBL-EBI Zn-finger, RING 7.7E-6
Pfam IPR000330 EMBL-EBI SNF2 related domain 2.4E-5
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 7.1E-10
Pfam IPR001841 EMBL-EBI Zn-finger, RING 7.7E-6
Sequence
Target Sequence 
>C. ELEGANS:188591_AT
attcctggaatcatattgtctgcgaagatgtcggaggctattatattgatgcgagagatt
ctagcagctgacccactaaacaaaatcattttattcacaactattgaacagacatccaca
caagtatgggaatatctaacaaagattctcaaacttgccaacctgccattcattgcacca
acacgagctacttgtggaaagaaagtcttcgattttgatgtatcggaagatacaaaggtt
cttctatgttccctatcactttgtgcaaatggtcttaatatgaccggagccaatcatatc
atctttttggatccaccacatcttcaatctgttctgaatcaggccatcggtagaattaat
cgattcggacagaaaagagacatgaatgtatatcatttgttagttgacggcagtctggac
attgaattaagagaaactgccaaaaagacatatcgtagagaagacgaacaaaaaggatgg
accattggcgacattcgtagtatgtttggaatcaattattatccagtgcaggcgaatatt
cgagccatagttggc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCCTGGAATCATATTGTCTGCGA 337 9 4219 Antisense
GAGAGATTCTAGCAGCTGACCCACT 603 391 4271 Antisense
AAGATTCTCAAACTTGCCAACCTGC 328 155 4360 Antisense
TGCCATTCATTGCACCAACACGAGC 142 587 4382 Antisense
CAAAGGTTCTTCTATGTTCCCTATC 360 193 4451 Antisense
ATGTTCCCTATCACTTTGTGCAAAT 115 47 4464 Antisense
GACCGGAGCCAATCATATCATCTTT 343 383 4500 Antisense
CATCTTTTTGGATCCACCACATCTT 329 213 4518 Antisense
CCACCACATCTTCAATCTGTTCTGA 475 273 4531 Antisense
GGATGGACCATTGGCGACATTCGTA 223 515 4693 Antisense
GGCGAATATTCGAGCCATAGTTGGC 595 539 4749 Antisense
Sequence Source Affymetrix Proprietary Database