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[188588_at] protease

Description W05G11.4 /REP_DB=WormBase Gene ID /WP=CE14630 /TR=O44903 /GB=AAC71148.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=protease
Cluster 6grp_total > 09      4grp_total > 34      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 79
1 2 3 4 range
A 56.9
(-1.4)
A 		36.5
(-3)
A 		114.8
(3)
A 		93.8
(1.4)
A 		78
B 69.4
(-0.9)
A 		115.8
(3)
A 		44.4
(-3)
A 		104.8
(2.1)
A 		71
C 51.1
(-2.8)
A 		110.3
(3)
A 		48.8
(-3)
A 		69.9
(-0.9)
P 		62
avr 59.1
(-3) 		87.5
(2.6) 		69.3
(-1) 		89.5
(3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 85.8
(3)
A 		35
(-3)
A 		44.8
(-1.8)
A 		54.5
(-0.7)
A 		62.5
(0.2)
A 		58.3
(-0.2)
A 		51
B 45.7
(-1.6)
A 		43.7
(-1.9)
A 		44.3
(-1.8)
P 		76
(3)
A 		36.4
(-3)
A 		46.8
(-1.4)
A 		40
C 56.1
(-0.8)
A 		46.9
(-3)
A 		72.4
(3)
A 		64.7
(1.2)
P 		53.8
(-1.4)
A 		50.8
(-2.1)
A 		26
avr 62.5
(2.3) 		41.9
(-3) 		53.8
(0.1) 		65.1
(3) 		50.9
(-0.7) 		52
(-0.4) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 20.7
(0.5)
A 		7.9
(-3)
A 		30
(3)
A 		13.7
(-1.4)
A 		22
B 22.1
(0.9)
A 		28.6
(3)
A 		10.3
(-3)
A 		22.3
(0.9)
A 		18
C 16.9
(-1.5)
A 		31.7
(3)
A 		17
(-1.4)
A 		11.8
(-3)
P 		20
avr 19.9
(0.5) 		22.7
(3) 		19.1
(-0.2) 		15.9
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 15.8
(1)
A 		12.5
(-1.2)
A 		9.8
(-3)
A 		13.8
(-0.4)
A 		18.9
(3)
A 		14.2
(-0.1)
A 		9
B 15.9
(0.3)
A 		14.6
(-0.5)
A 		10.8
(-3)
P 		20.1
(3)
A 		14.8
(-0.4)
A 		17.4
(1.3)
A 		9
C 20.8
(3)
A 		14.3
(-2)
A 		18.4
(1.2)
A 		13
(-3)
P 		17.4
(0.4)
A 		17
(0.1)
A 		8
avr 17.5
(3) 		13.8
(-1.9) 		13
(-3) 		15.6
(0.5) 		17
(2.3) 		16.2
(1.3) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188588_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8199
Sequence Type Exemplar sequence
Representative Public ID W05G11.4 NCBI
Target Description W05G11.4 /REP_DB=WormBase Gene ID /WP=CE14630 /TR=O44903 /GB=AAC71148.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064729(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064729 NCBI Caenorhabditis elegans W05G11.4 (protease) mRNA, complete cds. 11/11 None
SNAP00000017781 ENSEMBL cdna:SNAP chromosome:CEL140:III:35746:40693:-1 10/11 None
GENEFINDER00000017794 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:35746:39461:-1 10/11 None
W05G11.4 ENSEMBL cdna:known chromosome:CEL140:III:35454:39461:-1 gene:W05G11.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:35649-39461(-) 100.0 100.0
Public Domain and Genome References
Gene Title protease
Ensembl W05G11.4
Entrez Gene 189220 Entrez gene
SwissProt O44903 EMBL-EBI
WormBase CE14630 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4198 calpain activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC71148 Hypothetical protein W05G11.4 [Caenorhabditis elegans] ref|NP_497130.1| W05G11.4 [Caenorhabditis elegans] 0.0
blast CAC42267 Hypothetical protein T11A5.6 [Caenorhabditis elegans] emb|CAA96686.2| Hypothetical protein T11A5.6 [Caenorhabditis elegans] ref|NP_741604.1| T11A5.6 [Caenorhabditis elegans] 0.0
blast CAE72273 Hypothetical protein CBG19393 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.6E-27
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.5E-26
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 1.6E-27
Pfam IPR001300 EMBL-EBI Peptidase C2, calpain 2.5E-26
Sequence
Target Sequence 
>C. ELEGANS:188588_AT
ctgatctcatggtctgtgtgcacaaaatatctgaagatggacgtattggggaattaataa
aaatgtcctatgagatcgctgacaatcactttacaattgacgaattcttcctgccacgag
gagtctatcaagttgtctgtcacagtccacactccttagtgacaaatacaacaggcgtgg
tgaatataattgtgcacacacgttttccaatatttggtgaaagtgttccaatgtcacctc
ttacgagattggagtcgttgcatcgtgtaataattgaagaaggagttgtacaaaatatta
aacttgacggagttgtcatcagaactttgaaccaaaagtttagaggatcgatcacgatgg
tagataattatatggaacaaaagtacttacatgtcaaagttgacaattctaaatcaatga
atgttcaatcttcacgagggtcccttctaatagccgacgttgttcctccgcgctctcgtc
aagtgatagcagttctatcgaccatcgatgattgcgcagaatataagactgccaattctt
actggctccgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTGATCTCATGGTCTGTGTGCACAA 252 237 1331 Antisense
GAGATCGCTGACAATCACTTTACAA 195 397 1402 Antisense
AATTCTTCCTGCCACGAGGAGTCTA 374 177 1433 Antisense
AAGTTGTCTGTCACAGTCCACACTC 630 155 1460 Antisense
GTGCACACACGTTTTCCAATATTTG 363 481 1522 Antisense
AAAGTGTTCCAATGTCACCTCTTAC 682 121 1550 Antisense
GATTGGAGTCGTTGCATCGTGTAAT 565 435 1577 Antisense
AATGTTCAATCTTCACGAGGGTCCC 218 163 1750 Antisense
TTCTAATAGCCGACGTTGTTCCTCC 190 665 1775 Antisense
TCCGCGCTCTCGTCAAGTGATAGCA 56 597 1797 Antisense
ACTGCCAATTCTTACTGGCTCCGTA 234 97 1858 Antisense
Sequence Source Affymetrix Proprietary Database