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[188584_s_at]

Description F14B4.2 /REP_DB=WormBase Gene ID /WP=CE05628 /TR=Q19440 /GB=CAA99826.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=hexokinase
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 99      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 86      6grp_total > 94      4grp_total > 54      6grp_highest_sub > In      6grp_total_sub > Hat      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 783
1 2 3 4 range
A 1198.6
(3)
P 		641.9
(-1.5)
P 		657.6
(-1.4)
P 		454
(-3)
P 		745
B 1236.1
(3)
P 		659.8
(-2.2)
P 		692.3
(-1.9)
P 		570.6
(-3)
P 		666
C 1236.8
(3)
P 		739.5
(-0.8)
P 		689.5
(-1.2)
P 		453.7
(-3)
P 		783
avr 1223.8
(3) 		680.4
(-1.5) 		679.8
(-1.5) 		492.8
(-3) 		731

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 698
1 2 3 4 5 6 range
A 630.7
(-3)
P 		994.1
(0.6)
P 		769.8
(-1.6)
P 		1036.7
(1)
P 		1242.3
(3)
P 		1155.5
(2.1)
P 		612
B 630.5
(-3)
P 		897.8
(-0.4)
P 		1121.2
(1.7)
P 		1009.3
(0.7)
P 		1252.2
(3)
P 		1045.6
(1)
P 		622
C 961.5
(-1.4)
P 		917.3
(-1.9)
P 		1176.7
(1.2)
P 		825.1
(-3)
P 		1328.4
(3)
P 		1207.2
(1.6)
P 		503
avr 740.9
(-3) 		936.4
(-0.8) 		1022.6
(0.2) 		957
(-0.6) 		1274.3
(3) 		1136.1
(1.4) 		533
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 370
1 2 3 4 range
A 436.4
(3)
P 		139.3
(-1.8)
P 		171.9
(-1.3)
P 		66.5
(-3)
P 		370
B 394.2
(3)
P 		163
(-2.1)
P 		160.4
(-2.1)
P 		121.6
(-3)
P 		273
C 409.4
(3)
P 		212.6
(-0.5)
P 		240.3
(-0)
P 		76.5
(-3)
P 		333
avr 413.3
(3) 		171.6
(-1.5) 		190.9
(-1.1) 		88.2
(-3) 		325

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 392
1 2 3 4 5 6 range
A 116.3
(-3)
P 		353.5
(2.5)
P 		167.7
(-1.8)
P 		262.9
(0.4)
P 		374.9
(3)
P 		281.3
(0.8)
P 		259
B 219.4
(-3)
P 		298.8
(-1.4)
P 		272.3
(-1.9)
P 		267.3
(-2)
P 		508.7
(3)
P 		389.9
(0.5)
P 		289
C 356.4
(1.3)
P 		279.9
(-0.4)
P 		299.5
(0)
P 		165.9
(-3)
P 		429.8
(3)
P 		404.1
(2.4)
P 		264
avr 230.7
(-3) 		310.7
(-0.7) 		246.5
(-2.5) 		232
(-3) 		437.8
(3) 		358.4
(0.7) 		207
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188584_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2303
Sequence Type Exemplar sequence
Representative Public ID F14B4.2 NCBI
Target Description F14B4.2 /REP_DB=WormBase Gene ID /WP=CE05628 /TR=Q19440 /GB=CAA99826.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=hexokinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025936(11), NM_001025935(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025936 NCBI Caenorhabditis elegans F14B4.2b (hexokinase) mRNA, complete cds. 11/11 None
NM_001025935 NCBI Caenorhabditis elegans F14B4.2a (hexokinase) mRNA, complete cds. 11/11 None
SNAP00000033141 ENSEMBL cdna:SNAP chromosome:CEL140:I:9280015:9281904:-1 11/11 None
GENEFINDER00000033143 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9280015:9281904:-1 11/11 None
F14B4.2b ENSEMBL cdna:known chromosome:CEL140:I:9279567:9286812:-1 gene:F14B4.2 11/11 None
F14B4.2a.2 ENSEMBL cdna:known chromosome:CEL140:I:9280015:9281920:-1 gene:F14B4.2 11/11 None
F14B4.2a.1 ENSEMBL cdna:known chromosome:CEL140:I:9280015:9281918:-1 gene:F14B4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9279945-9281835(-) 100.0 100.0
Public Domain and Genome References
Ensembl F14B4.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAH04733 Hypothetical protein F14B4.2b [Caenorhabditis elegans] ref|NP_001021107.1| F14B4.2b [Caenorhabditis elegans] 0.0
blast CAA99826 Hypothetical protein F14B4.2a [Caenorhabditis elegans] ref|NP_001021106.1| F14B4.2a [Caenorhabditis elegans] 0.0
blast CAE72321 Hypothetical protein CBG19465 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001312 EMBL-EBI Hexokinase 2.1E-109
Pfam IPR001312 EMBL-EBI Hexokinase 7.0E-92
Pfam IPR001312 EMBL-EBI Hexokinase 3.2E-110
Pfam IPR001312 EMBL-EBI Hexokinase 7.0E-92
Sequence
Target Sequence 
>C. ELEGANS:188584_S_AT
gatttccttcgcaccgaatgggatgaagttgtggatcgtgagagcatcaacccaggacaa
catctctatgagaagatgatctccggaatgtatatgggagagtgtgcccgagttgttctc
gaagatttggcaaagcaaggattgctgttcggtggaaactcggatgctatttcggttcct
cattgctttccaacaaagtttgtatcggaaattgatagtgatcttcttgaggatgatgat
cgaacattccaaaagacttatcaaattctcgaggatattggtgttgagatgatcactgcg
aatgattgtgccaatgtggcgtacgtgtgtagtttgatttcaactcgtgctgcccatctg
acagccgctggaatcgctatgttgctcaatcgaatgaacaagaaacatgtgactgtcgga
gttgatggatctgtatatcgtttccatccaacttatccaacacttcttgatgccaaaatt
ggagaattgattgttggagacattgagtataaattgatgctctctgaagatggatctgga
cgtggagctgctctcgttgctgctgttgccactcgactgaaagag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTTCCTTCGCACCGAATGGGATG 238 425 886 Antisense
GTGGATCGTGAGAGCATCAACCCAG 5 491 916 Antisense
ATCAACCCAGGACAACATCTCTATG 265 25 931 Antisense
GAGAGTGTGCCCGAGTTGTTCTCGA 573 395 983 Antisense
GGATTGCTGTTCGGTGGAAACTCGG 693 507 1024 Antisense
AACTCGGATGCTATTTCGGTTCCTC 234 69 1042 Antisense
GTTCCTCATTGCTTTCCAACAAAGT 300 441 1060 Antisense
AGCCGCTGGAATCGCTATGTTGCTC 492 83 1248 Antisense
TATCGTTTCCATCCAACTTATCCAA 412 659 1321 Antisense
ACTTATCCAACACTTCTTGATGCCA 423 103 1336 Antisense
TGCTGTTGCCACTCGACTGAAAGAG 253 573 1446 Antisense
Sequence Source Affymetrix Proprietary Database