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[188564_at] F-spondin

Description F10E7.4 /REP_DB=WormBase Gene ID /WP=CE04359 /TR=Q19305 /GB=AAA82427.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=F-spondin
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 15      4grp_highest_8_8 > 42      4grp_high_8_8 > 49      6grp_highest_nonorm > 80      6grp_total > 04      4grp_total > 54      6grp_highest_sub > De      6grp_high1_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 420
1 2 3 4 range
A 433
(3)
P 		244.5
(-1.5)
P 		267.6
(-0.9)
P 		179.9
(-3)
P 		253
B 488.3
(3)
P 		276.1
(-1.4)
P 		286
(-1.2)
P 		199.5
(-3)
P 		289
C 553.9
(3)
P 		307.7
(-0.5)
P 		332.2
(-0.2)
P 		133.5
(-3)
P 		420
avr 491.7
(3) 		276.1
(-1) 		295.3
(-0.7) 		171
(-3) 		321

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 238
1 2 3 4 5 6 range
A 353
(3)
P 		342.2
(2.7)
P 		190.5
(-1.1)
P 		193.2
(-1)
P 		168.8
(-1.6)
P 		114.9
(-3)
P 		238
B 319.4
(3)
P 		262.5
(0.9)
P 		158.3
(-3)
P 		158.3
(-3)
P 		209.1
(-1.1)
P 		179.4
(-2.2)
P 		161
C 307.5
(3)
P 		261.9
(0.7)
P 		201.2
(-2.3)
P 		301
(2.7)
P 		198.7
(-2.5)
P 		188.2
(-3)
P 		119
avr 326.6
(3) 		288.9
(1.6) 		183.3
(-2.2) 		217.5
(-0.9) 		192.2
(-1.9) 		160.8
(-3) 		166
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 161
1 2 3 4 range
A 157.6
(3)
P 		53.1
(-1.8)
P 		70
(-1)
P 		26.3
(-3)
P 		131
B 155.7
(3)
P 		68.2
(-1.6)
P 		66.3
(-1.7)
P 		42.5
(-3)
P 		113
C 183.3
(3)
P 		88.5
(-0.5)
P 		115.8
(0.5)
P 		22.5
(-3)
P 		161
avr 165.5
(3) 		69.9
(-1.2) 		84
(-0.6) 		30.4
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 65.1
(-0.6)
P 		121.7
(3)
P 		41.5
(-2.1)
P 		49
(-1.7)
P 		50.9
(-1.5)
P 		28
(-3)
P 		94
B 111.2
(3)
P 		87.4
(1)
P 		38.4
(-3)
P 		41.9
(-2.7)
P 		85
(0.8)
P 		66.9
(-0.7)
P 		73
C 114
(3)
P 		79.9
(-0.3)
P 		51.2
(-3)
P 		60.5
(-2.1)
P 		64.3
(-1.7)
P 		63
(-1.9)
P 		63
avr 96.8
(3) 		96.3
(2.9) 		43.7
(-3) 		50.5
(-2.2) 		66.7
(-0.4) 		52.6
(-2) 		53
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188564_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5187
Sequence Type Exemplar sequence
Representative Public ID F10E7.4 NCBI
Target Description F10E7.4 /REP_DB=WormBase Gene ID /WP=CE04359 /TR=Q19305 /GB=AAA82427.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=F-spondin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063072(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063072 NCBI Caenorhabditis elegans F10E7.4 (F-spondin) mRNA, complete cds. 11/11 None
SNAP00000000731 ENSEMBL cdna:SNAP chromosome:CEL140:II:7116567:7120436:1 11/11 None
GENEFINDER00000000742 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7116567:7120436:1 11/11 None
F10E7.4 ENSEMBL cdna:known chromosome:CEL140:II:7116501:7120488:1 gene:F10E7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7116471-7120432(+) 100.0 100.0
Public Domain and Genome References
Gene Title F-spondin
Ensembl F10E7.4
Entrez Gene 174169 Entrez gene
SwissProt Q19305 EMBL-EBI
WormBase CE32368 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1629572_A_AT dm
DROSGENOME1:153368_AT dm
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA82427 Hypothetical protein F10E7.4 [Caenorhabditis elegans] ref|NP_495473.2| F10E7.4 [Caenorhabditis elegans] 0.0
blast CAE67546 Hypothetical protein CBG13071 [Caenorhabditis briggsae] 0.0
blast EAA00407 ENSANGP00000008856 [Anopheles gambiae str. PEST] ref|XP_320745.2| ENSANGP00000008856 [Anopheles gambiae str. PEST] 1.0E-146
Protein Domains
Database ID Description E-Value
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 2.1E-12
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 3.2E-10
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 2.1E-10
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 6.1E-11
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 5.0E-10
Pfam IPR002861 EMBL-EBI Reeler region 4.2E-74
Pfam IPR002223 EMBL-EBI Pancreatic trypsin inhibitor (Kunitz) 1.4E-25
Pfam IPR009465 EMBL-EBI Spondin, N-terminal 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:188564_AT
caaacaagctggtcagtgcgcaggaaatttccctcgttactggtacaatcacgaaaaaac
tcaatgcgaacgattcattttcactggatgcaaaggaaatcgaaatcagtttgaaaccga
agaagaatgcaaacaaatatgtcttccgggatatgaaaaatcaaaatcattgattccaaa
taaccaacttcttgaagattttggaaatacagaagtggatgatggtggtgaaagagttga
ttgtgaagtttcaaagtggacagcatggggatcttgctctgtgagctgtggtagaggaaa
gaaatcgagatcaagacacgttgtgaagttagcaagaaatggtggacatcaatgttctga
acatttaatgcaggaattgcaatgcagattgaggccttgccctgtaaaattgacttgtca
agttggtccttggtcccgttggtctccctgctctgtttcatgtggtgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAACAAGCTGGTCAGTGCGCAGGA 538 189 1845 Antisense
GTGCGCAGGAAATTTCCCTCGTTAC 683 475 1860 Antisense
TTTCCCTCGTTACTGGTACAATCAC 706 669 1872 Antisense
GAACGATTCATTTTCACTGGATGCA 23 349 1912 Antisense
TGCAAACAAATATGTCTTCCGGGAT 40 575 1972 Antisense
GGACAGCATGGGGATCTTGCTCTGT 68 527 2102 Antisense
GGGATCTTGCTCTGTGAGCTGTGGT 585 495 2112 Antisense
CAATGCAGATTGAGGCCTTGCCCTG 569 181 2224 Antisense
GGCCTTGCCCTGTAAAATTGACTTG 499 541 2237 Antisense
CTTGTCAAGTTGGTCCTTGGTCCCG 430 217 2258 Antisense
TCCCTGCTCTGTTTCATGTGGTGAA 335 597 2289 Antisense
Sequence Source Affymetrix Proprietary Database