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[188560_at]

Description C06E7.6 /REP_DB=WormBase Gene ID /WP=CE03962 /GEN=spe-27 /TR=SW:P54218 /GB=AAA82283.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 98      4grp_total > 47      6grp_cgc > 79      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 21.1
(3)
A 		5.8
(-3)
A 		7.8
(-2.2)
A 		6.2
(-2.8)
A 		15
B 24.4
(3)
A 		4
(-2.6)
A 		2.5
(-3)
A 		3.1
(-2.8)
A 		22
C 21.1
(3)
A 		8.9
(-1.6)
A 		5.3
(-3)
A 		8.8
(-1.7)
A 		16
avr 22.2
(3) 		6.2
(-2.6) 		5.2
(-3) 		6
(-2.7) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 71
1 2 3 4 5 6 range
A 13.5
(-2)
A 		1.7
(-3)
A 		23.1
(-1.2)
A 		1.5
(-3)
A 		25.4
(-1)
A 		72.3
(3)
A 		71
B 8.6
(-2.3)
A 		6
(-2.7)
A 		7.5
(-2.4)
A 		4
(-3)
A 		41.2
(3)
A 		32.3
(1.6)
M 		37
C 8.2
(-2)
A 		10.4
(-1.7)
A 		1.6
(-3)
A 		8.1
(-2.1)
A 		43.1
(3)
A 		33
(1.5)
A 		42
avr 10.1
(-2.2) 		6
(-2.8) 		10.7
(-2.1) 		4.5
(-3) 		36.6
(1.7) 		45.9
(3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 7.7
(3)
A 		1.2
(-2.7)
A 		2
(-2)
A 		0.9
(-3)
A 		7
B 7.8
(3)
A 		1
(-2.7)
A 		0.6
(-3)
A 		0.7
(-2.9)
A 		7
C 7
(3)
A 		2.5
(-1.9)
A 		1.8
(-2.7)
A 		1.5
(-3)
A 		6
avr 7.5
(3) 		1.6
(-2.4) 		1.5
(-2.5) 		1
(-3) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 2.5
(-2.3)
A 		0.6
(-2.9)
A 		5
(-1.4)
A 		0.4
(-3)
A 		7.7
(-0.5)
A 		17.6
(3)
A 		17
B 3
(-2.3)
A 		2
(-2.7)
A 		1.8
(-2.7)
A 		1.1
(-3)
A 		16.7
(3)
A 		12.1
(1.2)
M 		16
C 3.1
(-1.8)
A 		3.2
(-1.8)
A 		0.4
(-3)
A 		1.6
(-2.5)
A 		13.9
(3)
A 		11
(1.7)
A 		14
avr 2.9
(-2.1) 		1.9
(-2.6) 		2.4
(-2.3) 		1
(-3) 		12.8
(2.6) 		13.6
(3) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188560_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10852
Sequence Type Exemplar sequence
Representative Public ID C06E7.6 NCBI
Target Description C06E7.6 /REP_DB=WormBase Gene ID /WP=CE03962 /GEN=spe-27 /TR=SW:P54218 /GB=AAA82283.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068474(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068474 NCBI Caenorhabditis elegans defective SPErmatogenesis family member (spe-27) (spe-27) mRNA, complete cds. 11/11 None
SNAP00000039820 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5864530:5865405:-1 10/11 None
GENEFINDER00000039822 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5864530:5870206:-1 10/11 None
C06E7.6 ENSEMBL cdna:known chromosome:CEL140:IV:5864530:5865860:-1 gene:C06E7.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5864535-5865866(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol spe-27
Ensembl C06E7.6
Entrez Gene 177357 Entrez gene
SwissProt P54218 EMBL-EBI
WormBase CE03962 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82284 Serpentine receptor, class v protein 17 [Caenorhabditis elegans] ref|NP_500876.2| Serpentine Receptor, class V family member (srv-17) [Caenorhabditis elegans] 1.0E-165
blast AAD12795 Serpentine receptor, class v protein 22 [Caenorhabditis elegans] ref|NP_500881.1| Serpentine Receptor, class V family member (srv-22) [Caenorhabditis elegans] 3.0E-91
blast AAD12792 Serpentine receptor, class v protein 19 [Caenorhabditis elegans] ref|NP_500878.1| Serpentine Receptor, class V family member (srv-19) [Caenorhabditis elegans] 1.0E-90
blast AAA82283 Defective spermatogenesis protein 27 [Caenorhabditis elegans] ref|NP_500875.1| defective SPErmatogenesis family member (spe-27) [Caenorhabditis elegans] sp|P54218|SPE27_CAEEL Spermatocyte protein spe-27 precursor gb|AAC47276.1| SPE-27 5.0E-75
blast AAA82283 Defective spermatogenesis protein 27 [Caenorhabditis elegans] ref|NP_500875.1| defective SPErmatogenesis family member (spe-27) [Caenorhabditis elegans] sp|P54218|SPE27_CAEEL Spermatocyte protein spe-27 precursor gb|AAC47276.1| SPE-27 5.0E-44
blast CAE72639 Hypothetical protein CBG19841 [Caenorhabditis briggsae] 1.0E-41
blast CAE72639 Hypothetical protein CBG19841 [Caenorhabditis briggsae] 6.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR005040 EMBL-EBI Caenorhabditis protein of unknown function DUF280 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000039822 7 24-46,59-79,94-113,148-170,190-212,232-254,274-291
Sequence
Target Sequence 
>C. ELEGANS:188560_AT
tttgcatattcatgttactcgacaaaaactgaaaacaattttgacataaacgacgtggag
aataaagcgtgccaatatggatgtgggtttgaaaatgtcagaagaacttcgtttagaaag
acatacgacatactgtacacctgctctgaatgttccacacttttcgatttgtgcatcaag
taccgtacttgtcaagatggttgtgttccagaaatgccagttcttccctatgatcaagca
aaggatgtgtcggcgagaattcgtcccccaatgaatccctgcttggatttggtcactgcg
tgcaattacgagtca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGCATATTCATGTTACTCGACAA 162 669 43 Antisense
GACATACGACATACTGTACACCTGC 529 369 162 Antisense
TACTGTACACCTGCTCTGAATGTTC 91 647 173 Antisense
TGTTCCACACTTTTCGATTTGTGCA 357 563 193 Antisense
GATTTGTGCATCAAGTACCGTACTT 41 427 208 Antisense
AGATGGTTGTGTTCCAGAAATGCCA 57 69 237 Antisense
CCAGTTCTTCCCTATGATCAAGCAA 652 271 259 Antisense
GATGTGTCGGCGAGAATTCGTCCCC 653 405 286 Antisense
CCCCCAATGAATCCCTGCTTGGATT 510 259 307 Antisense
TGCTTGGATTTGGTCACTGCGTGCA 341 579 322 Antisense
GGTCACTGCGTGCAATTACGAGTCA 304 503 333 Antisense
Sequence Source Affymetrix Proprietary Database