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[188557_at]

Description C35D10.9 /REP_DB=WormBase Gene ID /WP=CE01203 /GEN=ced-4 /TR=SW:P30429 /GB=AAA62564.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 97      4grp_highest_8_8 > 0      4grp_high_8_8 > 45      6grp_highest_nonorm > 92      6grp_total > 89      4grp_total > 51      6grp_highest_sub > In      6grp_cgc > 59      6grp_high1_sub > In      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 184
1 2 3 4 range
A 315.7
(0.8)
P 		374.2
(3)
P 		331.5
(1.4)
P 		218
(-3)
P 		156
B 190.2
(-3)
P 		292.1
(3)
P 		259.3
(1.1)
P 		255.1
(0.8)
P 		102
C 290.1
(1.8)
P 		299.6
(3)
P 		262.5
(-1.8)
P 		253.5
(-3)
P 		46
avr 265.3
(-1.3) 		322
(3) 		284.4
(0.2) 		242.2
(-3) 		80

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 228
1 2 3 4 5 6 range
A 196.9
(-3)
P 		347.7
(1)
P 		213.4
(-2.6)
P 		266.2
(-1.2)
P 		379.4
(1.8)
P 		424.5
(3)
P 		228
B 302
(-0.9)
P 		267.3
(-3)
P 		344.7
(1.6)
P 		343
(1.5)
P 		309.1
(-0.5)
P 		368.2
(3)
P 		101
C 266.3
(-1.6)
P 		383.2
(2.4)
P 		227
(-3)
P 		263.5
(-1.7)
P 		370.6
(2)
P 		399.5
(3)
P 		173
avr 255.1
(-3) 		332.7
(0.3) 		261.7
(-2.7) 		290.9
(-1.5) 		353
(1.1) 		397.4
(3) 		142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 114.9
(3)
P 		81.2
(0.6)
P 		86.7
(1)
P 		31.9
(-3)
P 		83
B 60.6
(-0.9)
P 		72.1
(3)
P 		60.1
(-1.1)
P 		54.4
(-3)
P 		18
C 96
(3)
P 		86.1
(1.9)
P 		91.5
(2.5)
P 		42.7
(-3)
P 		53
avr 90.5
(3) 		79.8
(1.6) 		79.4
(1.6) 		43
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 101
1 2 3 4 5 6 range
A 36.3
(-3)
P 		123.7
(3)
P 		46.5
(-2.3)
P 		67.5
(-0.9)
P 		114.5
(2.4)
P 		103.4
(1.6)
P 		87
B 105.1
(-0.6)
P 		89
(-2.4)
P 		83.7
(-3)
P 		90.8
(-2.2)
P 		125.6
(1.7)
P 		137.3
(3)
P 		54
C 98.7
(0.4)
P 		116.9
(1.8)
P 		57.8
(-2.6)
P 		53
(-3)
P 		119.9
(2)
P 		133.7
(3)
P 		81
avr 80
(-1.3) 		109.9
(1.6) 		62.7
(-3) 		70.4
(-2.3) 		120
(2.5) 		124.8
(3) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188557_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6552
Sequence Type Exemplar sequence
Representative Public ID C35D10.9 NCBI
Target Description C35D10.9 /REP_DB=WormBase Gene ID /WP=CE01203 /GEN=ced-4 /TR=SW:P30429 /GB=AAA62564.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026031(11), NM_001026032(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026031 NCBI Caenorhabditis elegans CEll Death abnormality family member (ced-4) (ced-4) mRNA, complete cds. 11/11 None
NM_001026032 NCBI Caenorhabditis elegans CEll Death abnormality family member (ced-4) (ced-4) mRNA, complete cds. 11/11 None
SNAP00000029542 ENSEMBL cdna:SNAP chromosome:CEL140:III:4852286:4856033:1 11/11 None
GENEFINDER00000029570 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4852286:4860647:1 10/11 None
C35D10.9 ENSEMBL cdna:known chromosome:CEL140:III:4852286:4855127:1 gene:C35D10.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4852285-4854918(+) 100.0 100.0
Public Domain and Genome References
Ensembl C35D10.9
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA48781 ced-4 [Caenorhabditis elegans] ref|NP_001021202.1| CEll Death abnormality family member (ced-4) [Caenorhabditis elegans] gb|AAA62564.1| Cell death abnormality protein 4, isoform a [Caenorhabditis elegans] 0.0
blast NP_001021203 CEll Death abnormality family member (ced-4) [Caenorhabditis elegans] gb|AAX22294.1| Cell death abnormality protein 4, isoform b [Caenorhabditis elegans] sp|P30429|CED4_CAEEL Cell death protein 0.0
blast Q60Z52 Cell death protein 4 emb|CAE71110.1| Hypothetical protein CBG17963 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002182 EMBL-EBI NB-ARC domain 1.1E-38
Pfam IPR002182 EMBL-EBI NB-ARC domain 2.9E-87
Pfam IPR001315 EMBL-EBI CARD interaction domain 8.6E-24
Pfam IPR001395 EMBL-EBI Aldo/keto reductase 1.1E-110
Pfam IPR002182 EMBL-EBI NB-ARC domain 1.0E-126
Pfam IPR002182 EMBL-EBI NB-ARC domain 3.4E-38
Pfam IPR002182 EMBL-EBI NB-ARC domain 2.9E-87
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000029542 2 561-583,590-612
GENEFINDER00000029570 6 552-574,587-609,614-636,787-809,1360-1379,1386-1408
Sequence
Target Sequence 
>C. ELEGANS:188557_AT
cttactcgtacaagtcactcgcaatggctcttcaaagatgtgttgaagttttgtcagatg
aggatcgaagtgctcttgctttcgcagttgtgatgcctcctggagttgatatacccgtca
agctatggtcatgtgttattccagttgatatttgttcaaatgaagaagaacaattggatg
atgaagttgcggatcggttgaaaagactcagcaaacgtggagctcttctcagtggaaaac
gaatgcccgttttgacattcaaaattgatcatattatccatatgttcttgaaacacgtcg
ttgatgcacaaactatcgccaatggaatctcaattctcgagcagcgtcttcttgaaatag
gaaacaataatgtatcagtaccggagcgacatataccatcacatttccaaaaattccgtc
gttcatcagccagtgagatgtatccaaaaactacagaagaaactgtgatccgtcctgaag
ac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTACTCGTACAAGTCACTCGCAAT 655 221 1115 Antisense
GTCACTCGCAATGGCTCTTCAAAGA 218 465 1128 Antisense
AGGATCGAAGTGCTCTTGCTTTCGC 677 55 1175 Antisense
TTGCTTTCGCAGTTGTGATGCCTCC 304 703 1190 Antisense
GCCTCCTGGAGTTGATATACCCGTC 573 281 1209 Antisense
ATACCCGTCAAGCTATGGTCATGTG 557 23 1225 Antisense
GCAAACGTGGAGCTCTTCTCAGTGG 563 317 1325 Antisense
ATTCTCGAGCAGCGTCTTCTTGAAA 688 9 1447 Antisense
GAGCGACATATACCATCACATTTCC 52 387 1498 Antisense
CAAAAATTCCGTCGTTCATCAGCCA 340 191 1522 Antisense
AGAAACTGTGATCCGTCCTGAAGAC 291 73 1572 Antisense
Sequence Source Affymetrix Proprietary Database