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[188555_s_at]

Description F14D12.4 /REP_DB=WormBase Gene ID /WP=CE04393 /GEN=mec-2 /TR=SW:Q27433 /GB=AAA82333.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 06      6grp_total > 08      4grp_total > 30      6grp_cgc > 11      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 260.6
(3)
P 		109.7
(-3)
P 		117.4
(-2.7)
A 		166.7
(-0.7)
P 		151
B 243.9
(3)
P 		147.1
(-0.6)
A 		83.5
(-3)
A 		183.9
(0.8)
P 		160
C 232.5
(3)
P 		176.9
(0.5)
P 		97.7
(-3)
M 		190.9
(1.1)
P 		135
avr 245.7
(3) 		144.6
(-1.1) 		99.5
(-3) 		180.5
(0.3) 		146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 168
1 2 3 4 5 6 range
A 202.9
(3)
P 		169
(1.1)
P 		97.5
(-3)
M 		127.8
(-1.3)
P 		148.7
(-0.1)
P 		113.3
(-2.1)
P 		105
B 225.6
(3)
P 		100.8
(-3)
P 		112.8
(-2.4)
P 		102.2
(-2.9)
P 		113.8
(-2.4)
P 		134.3
(-1.4)
P 		125
C 161
(2.5)
P 		121.2
(0.4)
P 		171
(3)
P 		107.8
(-0.3)
A 		113.5
(-0)
P 		57.4
(-3)
P 		114
avr 196.5
(3) 		130.3
(-1.2) 		127.1
(-1.4) 		112.6
(-2.3) 		125.3
(-1.5) 		101.7
(-3) 		95
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 76
1 2 3 4 range
A 94.9
(3)
P 		23.8
(-3)
P 		30.7
(-2.4)
A 		24.4
(-2.9)
P 		71
B 77.8
(3)
P 		36.3
(-1.3)
A 		19.4
(-3)
A 		39.2
(-1)
P 		58
C 77
(3)
P 		50.9
(-0.5)
P 		34.1
(-2.7)
M 		32.2
(-3)
P 		45
avr 83.2
(3) 		37
(-2) 		28.1
(-3) 		31.9
(-2.6) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 37.4
(-0.5)
P 		60.1
(3)
P 		21.2
(-3)
M 		32.4
(-1.3)
P 		44.9
(0.7)
P 		27.6
(-2)
P 		39
B 78.5
(3)
P 		33.6
(-2.2)
P 		27.4
(-3)
P 		27.1
(-3)
P 		46.2
(-0.8)
P 		50.1
(-0.3)
P 		51
C 59.7
(3)
P 		37
(-0.4)
P 		43.5
(0.6)
P 		21.7
(-2.6)
A 		36.7
(-0.4)
P 		19.2
(-3)
P 		41
avr 58.5
(3) 		43.6
(0.2) 		30.7
(-2.3) 		27.1
(-3) 		42.6
(-0) 		32.3
(-2) 		31
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188555_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16894
Sequence Type Exemplar sequence
Representative Public ID F14D12.4 NCBI
Target Description F14D12.4 /REP_DB=WormBase Gene ID /WP=CE04393 /GEN=mec-2 /TR=SW:Q27433 /GB=AAA82333.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171691(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171691 NCBI Caenorhabditis elegans MEChanosensory abnormality family member (mec-2) (mec-2) mRNA, complete cds. 11/11 A 
SNAP00000023047 ENSEMBL cdna:SNAP chromosome:CEL140:X:5582634:5586572:1 9/11 None
GENEFINDER00000023056 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5582634:5590238:1 10/11 None
F14D12.4a ENSEMBL cdna:known chromosome:CEL140:X:5575892:5590774:1 gene:F14D12.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029395 5/11 Cross Hyb Matching Probes None
NM_001029396 5/11 Cross Hyb Matching Probes None
F14D12.4b 5/11 Cross Hyb Matching Probes None
F14D12.4c 5/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180826 3 1 NM_171691 175614_at (11), 188555_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5575928-5587758(+) 98.82 98.82
Public Domain and Genome References
Ensembl F14D12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA82333 Mechanosensory abnormality protein 2, isoform a [Caenorhabditis elegans] ref|NP_741797.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] sp|Q27433|MEC2_CAEEL Mechanosensory protein 2 gb|AAA87552.1| MEC-2 gb|AAA87551.1| MEC-2 prf||2201490A stomatin-like protein 0.0
blast CAE68766 Hypothetical protein CBG14704 [Caenorhabditis briggsae] 0.0
blast AAM54192 Mechanosensory abnormality protein 2, isoform b [Caenorhabditis elegans] ref|NP_001024566.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] 1.0E-167
blast AAA82333 Mechanosensory abnormality protein 2, isoform a [Caenorhabditis elegans] ref|NP_741797.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] sp|Q27433|MEC2_CAEEL Mechanosensory protein 2 gb|AAA87552.1| MEC-2 gb|AAA87551.1| MEC-2 prf||2201490A stomatin-like protein 1.0E-158
blast AAA82333 Mechanosensory abnormality protein 2, isoform a [Caenorhabditis elegans] ref|NP_741797.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] sp|Q27433|MEC2_CAEEL Mechanosensory protein 2 gb|AAA87552.1| MEC-2 gb|AAA87551.1| MEC-2 prf||2201490A stomatin-like protein 1.0E-147
blast AAV28352 Mechanosensory abnormality protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024567.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] 1.0E-139
blast AAV28352 Mechanosensory abnormality protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024567.1| MEChanosensory abnormality family member (mec-2) [Caenorhabditis elegans] 1.0E-128
Protein Domains
Database ID Description E-Value
Pfam IPR001107 EMBL-EBI Band 7 protein 4.1E-82
Pfam IPR001107 EMBL-EBI Band 7 protein 4.1E-82
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741797.1 1 114-136
F14D12.4a 1 114-136
SNAP00000023047 1 40-62
GENEFINDER00000023056 1 40-62
Sequence
Target Sequence 
>C. ELEGANS:188555_S_AT
tgttcgtctaccagttcaacttcaaagagctatggctgccgaagctgaagcagctcgtga
agcaagagctaaggtaatcgtcgccgaaggagaacaaaaagcctctcgagctcttaaaga
ggcagctgaagtcattgctgaaagcccatctgctcttcagcttcgttatttgcagacact
gaacagtatttcagctgaaaagaactcaacgattatattcccgttcccaattgatcttct
cagtgcattcctccaacgaacaccgcctaaagttgaggagccaccgtctttaccgaaaaa
aatccgttcatgctgcttgtacaagtaccctgattgggtgcaaggaatggttggatctga
aggtggtggaggtcacggacattcccatggaggaggaggaggagggcttggatcgtcaca
gggagcattccatccatctcaagcaggttccggcccgagcaccacaaccaccagtggccg
tccgctgctccgcagcatgcgagaagcgcagtttcactccgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTCGTCTACCAGTTCAACTTCAA 353 561 861 Antisense
GAACAAAAAGCCTCTCGAGCTCTTA 594 351 952 Antisense
GCTGAAAGCCCATCTGCTCTTCAGC 61 295 997 Antisense
GCTCTTCAGCTTCGTTATTTGCAGA 271 301 1012 Antisense
AATTGATCTTCTCAGTGCATTCCTC 292 179 1089 Antisense
AAAAATCCGTTCATGCTGCTTGTAC 457 129 1158 Antisense
GGAGGAGGAGGGCTTGGATCGTCAC 358 515 1255 Antisense
GATCGTCACAGGGAGCATTCCATCC 143 419 1271 Antisense
ATTCCATCCATCTCAAGCAGGTTCC 455 7 1287 Antisense
TCCGCTGCTCCGCAGCATGCGAGAA 163 597 1341 Antisense
TGCGAGAAGCGCAGTTTCACTCCGC 8 585 1358 Antisense
Sequence Source Affymetrix Proprietary Database