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[188548_s_at] helicase /// ---

Description F53H1.1 /REP_DB=WormBase Gene ID /WP=CE17860 /CHR=4 /FEA=Sanger Annotation /DEF=helicase (ST.LOUIS) TR:O45076 protein_id:AAC02576.1
Cluster 6grp_high1_10_10 > 27      4grp_high_8_8 > 08      6grp_total > 35      4grp_total > 31      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 125.6
(3)
P 		75.5
(-1.2)
P 		53.2
(-3)
A 		68.4
(-1.7)
A 		72
B 80.5
(3)
P 		53
(-0.8)
P 		37.4
(-3)
A 		76.6
(2.5)
P 		43
C 138.4
(3)
P 		62.6
(-3)
P 		82.5
(-1.4)
P 		108.6
(0.6)
P 		76
avr 114.8
(3) 		63.7
(-2.4) 		57.7
(-3) 		84.5
(-0.2) 		57

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 37.7
(-3)
A 		105.8
(3)
P 		58.5
(-1.2)
P 		56.3
(-1.4)
P 		52
(-1.7)
P 		57
(-1.3)
P 		68
B 71.4
(0.4)
P 		61.5
(-0.9)
P 		91.2
(3)
P 		54.5
(-1.8)
P 		45.3
(-3)
P 		66.8
(-0.2)
P 		46
C 115.5
(3)
P 		59.4
(-1.1)
P 		59.6
(-1.1)
A 		33.5
(-3)
P 		78.7
(0.3)
P 		68.4
(-0.4)
P 		82
avr 74.9
(2.8) 		75.6
(3) 		69.8
(1.7) 		48.1
(-3) 		58.7
(-0.7) 		64.1
(0.5) 		28
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 37
1 2 3 4 range
A 45.7
(3)
P 		16.4
(-1.9)
P 		13.9
(-2.3)
A 		10
(-3)
A 		36
B 25.7
(3)
P 		13.1
(-1.4)
P 		8.7
(-3)
A 		16.3
(-0.3)
P 		17
C 45.8
(3)
P 		18
(-3)
P 		28.8
(-0.7)
P 		18.3
(-2.9)
P 		28
avr 39.1
(3) 		15.8
(-2.8) 		17.1
(-2.5) 		14.9
(-3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 36
1 2 3 4 5 6 range
A 7
(-3)
A 		37.6
(3)
P 		12.7
(-1.9)
P 		14.3
(-1.6)
P 		15.7
(-1.3)
P 		13.9
(-1.6)
P 		31
B 24.8
(2.9)
P 		20.5
(0.5)
P 		22.1
(1.4)
P 		14.4
(-3)
P 		18.4
(-0.7)
P 		24.9
(3)
P 		11
C 42.8
(3)
P 		18.1
(-1.1)
P 		15.2
(-1.6)
A 		6.7
(-3)
P 		25.5
(0.1)
P 		22.9
(-0.3)
P 		36
avr 24.9
(2.8) 		25.4
(3) 		16.7
(-0.8) 		11.8
(-3) 		19.9
(0.6) 		20.6
(0.9) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188548_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10721
Sequence Type Exemplar sequence
Representative Public ID F53H1.1 NCBI
Target Description F53H1.1 /REP_DB=WormBase Gene ID /WP=CE17860 /CHR=4 /FEA=Sanger Annotation /DEF=helicase (ST.LOUIS) TR:O45076 protein_id:AAC02576.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067662(11), NM_067661(11), NM_075651(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067662 NCBI Caenorhabditis elegans F53H1.1a (helicase) mRNA, complete cds. 11/11 A 
NM_067661 NCBI Caenorhabditis elegans F53H1.1b (helicase) mRNA, complete cds. 11/11 A 
NM_075651 NCBI Caenorhabditis elegans Y73B3B.4 (Y73B3B.4) mRNA, complete cds. 11/11 A 
SNAP00000013765 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1297406:1335883:-1 11/11 None
GENEFINDER00000013782 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1297400:1309644:-1 11/11 None
SNAP00000035180 ENSEMBL cdna:SNAP chromosome:CEL140:X:199121:212240:1 11/11 None
GENEFINDER00000035182 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:200239:207723:1 11/11 None
F53H1.1a ENSEMBL cdna:known chromosome:CEL140:IV:1297283:1309644:-1 gene:F53H1.1 11/11 A 
F53H1.1b ENSEMBL cdna:known chromosome:CEL140:IV:1297400:1309644:-1 gene:F53H1.1 11/11 A 
Y73B3B.4 ENSEMBL cdna:known chromosome:CEL140:X:199223:207784:1 gene:Y73B3B.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1303033-1309650(-) 92.89 100.0
Public Domain and Genome References
Gene Title helicase

Ensembl F53H1.1
Y73B3B.4
Entrez Gene 176943 Entrez gene
180379 Entrez gene
SwissProt Q965K2 EMBL-EBI
Q95XD7 EMBL-EBI
WormBase CE27427 Wormbase
CE29920 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262851_AT DEAD box RNA helicase, putative at
ATGENOME1:12047_AT DEAD box RNA helicase, putative at
CANINE:1602991_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 cfa
CANINE_2:CFAAFFX.2470.1.S1_S_AT similar to DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 cfa
DROSOPHILA_2:1638962_AT dm
DROSGENOME1:154064_AT dm
CHICKEN:GGA.10433.1.S1_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 gga
HG-U133_PLUS_2:202462_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HG-U133A:202462_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HG-U133A_2:202462_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HU35KSUBA:RC_AA256994_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
U133_X3P:G7662317_3P_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
U133_X3P:HS.123075.0.A1_3P_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HG-U133_PLUS_2:228039_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HG-U133B:228039_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HG-U95AV2:33237_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HG-U95E:88766_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
HU35KSUBD:RC_C21113_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 hs
MOUSE430_2:1457744_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOE430B:1457744_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MU11KSUBA:AA245372_S_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOE430A:1424570_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOUSE430A_2:1424570_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOUSE430_2:1424570_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOE430A:1424571_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOUSE430A_2:1424571_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOUSE430_2:1424571_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOE430A:1424569_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOUSE430A_2:1424569_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MOUSE430_2:1424569_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46 mm
MG-U74AV2:101423_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MG-U74BV2:115147_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MOE430B:1455103_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MOUSE430_2:1455103_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MG-U74BV2:164476_R_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MU19KSUBA:TC24340_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MU19KSUBB:TC28716_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MU19KSUBB:TC28716_G_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
MU19KSUBC:TC40777_AT DEAD (Asp-Glu-Ala-Asp) box polypeptide 46, mRNA (cDNA clone MGC:31579 IMAGE:4505095) mm
RG-U34A:U25746_AT RNA helicase rn
RG-U34A:U25746_G_AT RNA helicase rn
RAE230A:1387912_AT RNA helicase rn
RAT230_2:1387912_AT RNA helicase rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68386 Hypothetical protein F53H1.1 [Caenorhabditis elegans] ref|NP_500063.1| F53H1.1a [Caenorhabditis elegans] 0.0
blast CAE70203 Hypothetical protein CBG16678 [Caenorhabditis briggsae] 0.0
blast NP_500062 F53H1.1b [Caenorhabditis elegans] 0.0
blast AAK39375 Hypothetical protein Y73B3B.4 [Caenorhabditis elegans] ref|NP_508052.2| Y73B3B.4 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.9E-30
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.2E-86
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.7E-30
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.6E-50
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.9E-30
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.2E-86
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.9E-30
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.2E-41
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 4.2E-34
Pfam IPR006570 EMBL-EBI SPK 2.0E-44
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.7E-30
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 8.3E-52
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.7E-30
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.4E-34
Sequence
Target Sequence 
>C. ELEGANS:188548_S_AT
atgattgacgtactggcggcgaatagtggaaaagtcacaaatttacgaagagtgacgtat
ttggtgttggatgaagccgatcgaatgtttgataagggctttgagccgcaggtaggccag
aaaattaggaaaagttcaatatttagatttttgcccaaaacttgccaaatttatgttttt
gaaggtggagtacggcctatcatgaaagtggtgaacaatatccgtcccgacaagcagact
gtactcttctctgccacgtttccccgacacatggaggctctggcacgaaaagttctggat
aaacctgttgagattttggtcggtggaaaatctgtcgtatgtagtgacatcactcagaat
gcagtaatttgtgcagagcatcagaagtttttgaaattgctggaactgttgggaatgtac
tacgaggagggaagctcgattgtatttgtagataaacaggaaaaagctgatgatattgta
gatcaactcatgagaactggatataattcagttgctccattacatggaggaattgatcaa
catgatcgagattcatcgattgctgactttaaaactggtgtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGATTGACGTACTGGCGGCGAATA 646 45 1324 Antisense
GACGTACTGGCGGCGAATAGTGGAA 16 379 1330 Antisense
CGTATTTGGTGTTGGATGAAGCCGA 664 245 1379 Antisense
CGGTGGAAAATCTGTCGTATGTAGT 698 239 1644 Antisense
TAGTGACATCACTCAGAATGCAGTA 201 651 1665 Antisense
GCAGTAATTTGTGCAGAGCATCAGA 687 311 1684 Antisense
GCTGGAACTGTTGGGAATGTACTAC 149 295 1722 Antisense
GAGGGAAGCTCGATTGTATTTGTAG 626 401 1750 Antisense
GATCAACTCATGAGAACTGGATATA 667 421 1804 Antisense
GAGATTCATCGATTGCTGACTTTAA 400 397 1871 Antisense
GATTGCTGACTTTAAAACTGGTGTT 504 433 1881 Antisense
Sequence Source Affymetrix Proprietary Database