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[188545_s_at]

Description T09A5.10 /REP_DB=WormBase Gene ID /WP=CE18951 /GEN=lin-5 /TR=SW:P45970 /GB=CAA85342.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 66      4grp_highest_8_8 > 41      4grp_high_8_8 > 50      6grp_highest_nonorm > 57      6grp_total > 67      4grp_total > 53      6grp_highest_sub > Da      6grp_cgc > 38      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 897
1 2 3 4 range
A 2277.9
(3)
P 		2000.3
(0.7)
P 		1998.4
(0.7)
P 		1553.2
(-3)
P 		725
B 2103.6
(3)
P 		2087.1
(2.8)
P 		1990.4
(1.5)
P 		1656.8
(-3)
P 		447
C 2148.4
(3)
P 		1766.3
(0)
P 		1788.5
(0.2)
P 		1381.1
(-3)
P 		767
avr 2176.6
(3) 		1951.2
(0.9) 		1925.8
(0.7) 		1530.4
(-3) 		646

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1122
1 2 3 4 5 6 range
A 2673.7
(1.1)
P 		1939.7
(-3)
P 		1997.4
(-2.7)
P 		2203.4
(-1.5)
P 		2210.3
(-1.5)
P 		3011.2
(3)
P 		1072
B 2341
(-2.2)
P 		2250.9
(-3)
P 		2524.8
(-0.6)
P 		2299.7
(-2.6)
P 		2299.6
(-2.6)
P 		2942.7
(3)
P 		692
C 1889.5
(-3)
P 		2254.2
(-1)
P 		2439.8
(-0)
P 		2130.5
(-1.7)
P 		2108
(-1.8)
P 		3001.8
(3)
P 		1112
avr 2301.4
(-1.9) 		2148.3
(-3) 		2320.7
(-1.8) 		2211.2
(-2.5) 		2206
(-2.6) 		2985.2
(3) 		837
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 602
1 2 3 4 range
A 829.4
(3)
P 		434.2
(-0.9)
P 		522.4
(-0.1)
P 		227.4
(-3)
P 		602
B 670.8
(3)
P 		515.5
(0.1)
P 		461.2
(-1)
P 		353.1
(-3)
P 		318
C 711.2
(3)
P 		507.8
(0.4)
P 		623.3
(1.9)
P 		232.9
(-3)
P 		478
avr 737.1
(3) 		485.8
(-0.2) 		535.6
(0.4) 		271.1
(-3) 		466

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 669
1 2 3 4 5 6 range
A 492.9
(-1.8)
P 		689.8
(2.1)
P 		435
(-3)
P 		558.8
(-0.5)
P 		667
(1.7)
P 		733.1
(3)
P 		298
B 814.6
(-0.5)
P 		749.2
(-1.3)
P 		613.1
(-3)
P 		609.1
(-3)
P 		934.3
(1)
P 		1097.3
(3)
P 		488
C 700.3
(-0.2)
P 		687.8
(-0.3)
P 		621
(-1)
P 		428.4
(-3)
P 		682.1
(-0.4)
P 		1004.9
(3)
P 		577
avr 669.3
(-1) 		708.9
(-0.4) 		556.4
(-2.6) 		532.1
(-3) 		761.1
(0.3) 		945.1
(3) 		413
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188545_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4808
Sequence Type Exemplar sequence
Representative Public ID T09A5.10 NCBI
Target Description T09A5.10 /REP_DB=WormBase Gene ID /WP=CE18951 /GEN=lin-5 /TR=SW:P45970 /GB=CAA85342.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063253(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063253 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-5) (lin-5) mRNA, complete cds. 11/11 A 
SNAP00000029441 ENSEMBL cdna:SNAP chromosome:CEL140:II:7860581:7863347:1 11/11 None
GENEFINDER00000029448 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7860581:7863347:1 11/11 None
T09A5.10.1 ENSEMBL cdna:known chromosome:CEL140:II:7860514:7863484:1 gene:T09A5.10 11/11 None
T09A5.10.2 ENSEMBL cdna:known chromosome:CEL140:II:7860580:7863474:1 gene:T09A5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7860577-7863344(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-5
Ensembl T09A5.10
Entrez Gene 174267 Entrez gene
SwissProt P45970 EMBL-EBI
WormBase CE18951 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA85342 Hypothetical protein T09A5.10 [Caenorhabditis elegans] ref|NP_495654.1| abnormal cell LINeage family member (lin-5) [Caenorhabditis elegans] gb|AAF61239.1| LIN-5 [Caenorhabditis elegans] sp|P45970|LIN5_CAEEL Spindle apparatus protein lin-5 (Abnormal cell lineage protein 5) 0.0
blast CAB02893 Hypothetical protein F01G10.5 [Caenorhabditis elegans] ref|NP_501873.1| F01G10.5 [Caenorhabditis elegans] 1.0E-170
Sequence
Target Sequence 
>C. ELEGANS:188545_S_AT
cattcacaccatccggagtaaccaaggaaagagttggagtgctcactgccagaaacgaga
aagtgaagccacacctaaaatgctcgtacgcctctgaagttggaagtacgaattcgccat
ccgcagacgaggagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATTCACACCATCCGGAGTAACCAA 607 213 2273 Antisense
CACACCATCCGGAGTAACCAAGGAA 391 193 2277 Antisense
GGAAAGAGTTGGAGTGCTCACTGCC 588 525 2298 Antisense
GGAGTGCTCACTGCCAGAAACGAGA 338 517 2308 Antisense
GCCAGAAACGAGAAAGTGAAGCCAC 694 275 2320 Antisense
GTGAAGCCACACCTAAAATGCTCGT 4 483 2335 Antisense
ACACCTAAAATGCTCGTACGCCTCT 681 109 2343 Antisense
AAAATGCTCGTACGCCTCTGAAGTT 406 131 2349 Antisense
GCTCGTACGCCTCTGAAGTTGGAAG 484 299 2354 Antisense
TGGAAGTACGAATTCGCCATCCGCA 435 545 2373 Antisense
ATTCGCCATCCGCAGACGAGGAGAA 580 7 2384 Antisense
Sequence Source Affymetrix Proprietary Database