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[188544_at]

Description T09A5.10 /REP_DB=WormBase Gene ID /WP=CE18951 /GEN=lin-5 /TR=SW:P45970 /GB=CAA85342.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 77      6grp_high1_10_10 > 77      4grp_highest_8_8 > 50      4grp_high_8_8 > 32      6grp_highest_nonorm > 84      6grp_total > 77      4grp_total > 47      6grp_highest_sub > Da      6grp_cgc > 48      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 234
1 2 3 4 range
A 566.9
(3)
P 		468.4
(0.3)
P 		351.3
(-3)
P 		373.3
(-2.4)
P 		216
B 585.1
(3)
P 		359.3
(-3)
P 		374.5
(-2.6)
P 		398.2
(-2)
P 		226
C 434.5
(2.9)
P 		355.3
(-3)
P 		435.6
(3)
P 		377.9
(-1.3)
P 		80
avr 528.8
(3) 		394.3
(-2.5) 		387.1
(-2.8) 		383.1
(-3) 		146

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 606
1 2 3 4 5 6 range
A 523.8
(-1)
P 		516.9
(-1)
P 		348.7
(-3)
P 		575.9
(-0.4)
P 		589.5
(-0.2)
P 		864.7
(3)
P 		516
B 575
(-2.3)
P 		606.5
(-1.9)
P 		527
(-3)
P 		590.3
(-2.1)
P 		625.1
(-1.6)
P 		955
(3)
P 		428
C 508.5
(-3)
P 		529.6
(-2.5)
P 		618
(-0.7)
P 		632.4
(-0.3)
P 		571.6
(-1.6)
P 		788.6
(3)
P 		280
avr 535.8
(-2.4) 		551
(-2.1) 		497.9
(-3) 		599.5
(-1.4) 		595.4
(-1.4) 		869.4
(3) 		372
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 152
1 2 3 4 range
A 206.4
(3)
P 		101.7
(-1.1)
P 		91.8
(-1.5)
P 		54.6
(-3)
P 		152
B 186.6
(3)
P 		88.7
(-2.8)
P 		86.8
(-2.9)
P 		84.9
(-3)
P 		102
C 143.8
(2.5)
P 		102.1
(-0.4)
P 		151.8
(3)
P 		63.7
(-3)
P 		88
avr 178.9
(3) 		97.5
(-1.4) 		110.1
(-0.7) 		67.7
(-3) 		111

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 280
1 2 3 4 5 6 range
A 96.6
(-2.1)
P 		183.8
(1.8)
P 		75.9
(-3)
P 		146.1
(0.1)
P 		177.9
(1.5)
P 		210.5
(3)
P 		135
B 200.1
(-1.1)
P 		201.9
(-1.1)
P 		128
(-3)
P 		156.4
(-2.3)
P 		254
(0.3)
P 		356.1
(3)
P 		228
C 188.5
(-0.3)
P 		161.6
(-1.5)
P 		157.3
(-1.7)
P 		127.2
(-3)
P 		185
(-0.5)
P 		264
(3)
P 		137
avr 161.7
(-1.4) 		182.4
(-0.6) 		120.4
(-3) 		143.2
(-2.1) 		205.6
(0.3) 		276.9
(3) 		157
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188544_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4808
Sequence Type Exemplar sequence
Representative Public ID T09A5.10 NCBI
Target Description T09A5.10 /REP_DB=WormBase Gene ID /WP=CE18951 /GEN=lin-5 /TR=SW:P45970 /GB=CAA85342.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063253(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063253 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-5) (lin-5) mRNA, complete cds. 11/11 A 
SNAP00000029441 ENSEMBL cdna:SNAP chromosome:CEL140:II:7860581:7863347:1 11/11 None
GENEFINDER00000029448 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7860581:7863347:1 11/11 None
T09A5.10.1 ENSEMBL cdna:known chromosome:CEL140:II:7860514:7863484:1 gene:T09A5.10 11/11 None
T09A5.10.2 ENSEMBL cdna:known chromosome:CEL140:II:7860580:7863474:1 gene:T09A5.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7860577-7863344(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-5
Ensembl T09A5.10
Entrez Gene 174267 Entrez gene
SwissProt P45970 EMBL-EBI
WormBase CE18951 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA85342 Hypothetical protein T09A5.10 [Caenorhabditis elegans] ref|NP_495654.1| abnormal cell LINeage family member (lin-5) [Caenorhabditis elegans] gb|AAF61239.1| LIN-5 [Caenorhabditis elegans] sp|P45970|LIN5_CAEEL Spindle apparatus protein lin-5 (Abnormal cell lineage protein 5) 0.0
blast CAB02893 Hypothetical protein F01G10.5 [Caenorhabditis elegans] ref|NP_501873.1| F01G10.5 [Caenorhabditis elegans] 1.0E-170
Sequence
Target Sequence 
>C. ELEGANS:188544_AT
gattcaacgtctcgagcacgaagttgcagatcttcgagagaaggaaagcgccgagattaa
gaaatccacctggatgggtgagagaatcaaaatcttccaacaagctccaatgagaggttc
gagaatcagatgcggttcgccagacagcttgccagacttcctcgccgataatggaccaac
aattaccgaagaagaattgcgagcacagcttgccacgttcccaagaaaatcgattgctcc
tagtgtggacgacaacgaattcgataaaggaacaccgattggattcaagtcgaatatgta
cgatggacgtgagtcaatttgctctttggatttcttcgaccggtctagtcttcaacgttc
gtcgatgcgatcggaatctatccaattggcatcgccatcatcagctggagagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCAACGTCTCGAGCACGAAGTT 95 431 1851 Antisense
AAATCTTCCAACAAGCTCCAATGAG 519 117 1940 Antisense
CAGATGCGGTTCGCCAGACAGCTTG 54 207 1977 Antisense
AAGAATTGCGAGCACAGCTTGCCAC 529 151 2042 Antisense
CAGCTTGCCACGTTCCCAAGAAAAT 372 203 2056 Antisense
GAAAATCGATTGCTCCTAGTGTGGA 628 353 2075 Antisense
TTTGCTCTTTGGATTTCTTCGACCG 532 667 2168 Antisense
TTTCTTCGACCGGTCTAGTCTTCAA 39 673 2181 Antisense
GTCTTCAACGTTCGTCGATGCGATC 75 469 2198 Antisense
GAATCTATCCAATTGGCATCGCCAT 301 329 2224 Antisense
GCATCGCCATCATCAGCTGGAGAGT 587 309 2239 Antisense
Sequence Source Affymetrix Proprietary Database