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[188540_at]

Description T19C3.8 /REP_DB=WormBase Gene ID /WP=CE02878 /GEN=fem-2 /TR=SW:P49594 /GB=AAC46598.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 98      4grp_highest_8_8 > 29      4grp_high_8_8 > 07      6grp_highest_nonorm > 91      6grp_total > 98      4grp_total > 0      6grp_highest_sub > In      6grp_cgc > 69      6grp_high1_sub > In      6grp_cgc_sub > Da      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 214
1 2 3 4 range
A 273.3
(-0.5)
P 		183.4
(-3)
P 		245.4
(-1.3)
P 		397
(3)
P 		214
B 229.1
(-2.2)
P 		223.2
(-3)
P 		244
(-0.2)
P 		268.3
(3)
P 		45
C 252.7
(-1.2)
P 		234.2
(-3)
P 		280.1
(1.4)
P 		296.1
(3)
P 		62
avr 251.7
(-0.9) 		213.6
(-3) 		256.5
(-0.6) 		320.5
(3) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 200
1 2 3 4 5 6 range
A 138.6
(-3)
P 		200.7
(-0.5)
P 		194.6
(-0.7)
P 		204.2
(-0.3)
P 		259.9
(2)
P 		285.6
(3)
P 		147
B 181.4
(-2.5)
P 		168.5
(-3)
P 		190.5
(-2.2)
P 		211.7
(-1.4)
P 		327.4
(2.9)
P 		330.9
(3)
P 		162
C 210.6
(-0.3)
P 		131
(-3)
P 		231.2
(0.4)
P 		168.9
(-1.7)
P 		262.4
(1.4)
P 		310.2
(3)
P 		179
avr 176.9
(-2.6) 		166.7
(-3) 		205.4
(-1.4) 		194.9
(-1.8) 		283.2
(1.9) 		308.9
(3) 		142
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 99.5
(3)
P 		39.8
(-3)
P 		64.1
(-0.6)
P 		58.1
(-1.2)
P 		60
B 73.1
(3)
P 		55.1
(-3)
P 		56.5
(-2.5)
P 		57.2
(-2.3)
P 		18
C 83.7
(1.3)
P 		67.3
(-0.8)
P 		97.6
(3)
P 		49.9
(-3)
P 		48
avr 85.4
(3) 		54.1
(-3) 		72.7
(0.6) 		55.1
(-2.8) 		31

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 25.6
(-3)
P 		71.4
(2.2)
P 		42.4
(-1.1)
P 		51.8
(-0)
P 		78.4
(3)
P 		69.5
(2)
P 		53
B 63.1
(-1.8)
P 		56.1
(-2.3)
P 		46.2
(-3)
P 		56.1
(-2.3)
P 		133
(3)
P 		123.4
(2.3)
P 		87
C 78.1
(0.8)
P 		40
(-2.5)
P 		58.8
(-0.9)
P 		34
(-3)
P 		84.9
(1.4)
P 		103.8
(3)
P 		70
avr 55.6
(-2) 		55.8
(-2) 		49.1
(-2.8) 		47.3
(-3) 		98.8
(3) 		98.9
(3) 		52
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188540_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8325
Sequence Type Exemplar sequence
Representative Public ID T19C3.8 NCBI
Target Description T19C3.8 /REP_DB=WormBase Gene ID /WP=CE02878 /GEN=fem-2 /TR=SW:P49594 /GB=AAC46598.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064823(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064823 NCBI Caenorhabditis elegans FEMinization of XX and XO animals family member (fem-2) (fem-2) mRNA, complete cds. 11/11 None
T19C3.8 ENSEMBL cdna:known chromosome:CEL140:III:632026:634623:-1 gene:T19C3.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:632405-634569(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol fem-2
Ensembl T19C3.8
Entrez Gene 175217 Entrez gene
SwissProt P49594 EMBL-EBI
WormBase CE02878 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.23714.1.S1_AT similar to Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaM-kinase phosphatase) (CaMKPase) (Partner of PIX 2) (hFEM-2) (Protein phosphatase 1F) cfa
HG-U95AV2:37384_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133A:37384_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133_PLUS_2:37384_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133A_2:37384_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133_PLUS_2:203063_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133A:203063_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133A_2:203063_AT protein phosphatase 1F (PP2C domain containing) hs
HUGENEFL:D13640_AT protein phosphatase 1F (PP2C domain containing) hs
U133_X3P:G7661861_3P_S_AT protein phosphatase 1F (PP2C domain containing) hs
U133_X3P:HS2.278441.3.S1_3P_S_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133_PLUS_2:1555470_A_AT protein phosphatase 1F (PP2C domain containing) hs
HG-U133_PLUS_2:207758_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U133A:207758_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U133A_2:207758_AT Protein phosphatase 1F (PP2C domain containing) hs
HU35KSUBC:RC_N72086_AT Protein phosphatase 1F (PP2C domain containing) hs
U133_X3P:G13376588_3P_X_AT Protein phosphatase 1F (PP2C domain containing) hs
U133_X3P:G13376588_3P_AT Protein phosphatase 1F (PP2C domain containing) hs
U133_X3P:HS2.278441.2.S1_3P_X_AT Protein phosphatase 1F (PP2C domain containing) hs
U133_X3P:HS.292284.0.A1_3P_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U133_PLUS_2:1555091_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U133_PLUS_2:240115_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U133B:240115_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U95B:46430_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U95D:85070_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U95C:56335_AT Protein phosphatase 1F (PP2C domain containing) hs
HG-U95C:60376_AT Protein phosphatase 1F (PP2C domain containing) hs
HU35KSUBD:RC_AA609591_AT Protein phosphatase 1F (PP2C domain containing) hs
MG-U74BV2:106649_AT protein phosphatase 1F (PP2C domain containing) mm
MOE430B:1430631_AT protein phosphatase 1F (PP2C domain containing) mm
MOUSE430_2:1430631_AT protein phosphatase 1F (PP2C domain containing) mm
MOE430B:1454934_AT protein phosphatase 1F (PP2C domain containing) mm
MOUSE430_2:1454934_AT protein phosphatase 1F (PP2C domain containing) mm
MG-U74BV2:164959_R_AT Protein phosphatase 1F (PP2C domain containing) (Ppm1f), mRNA mm
MU19KSUBB:TC33717_AT Protein phosphatase 1F (PP2C domain containing) (Ppm1f), mRNA mm
RAE230A:1370552_AT protein phosphatase 1F (PP2C domain containing) rn
RAT230_2:1370552_AT protein phosphatase 1F (PP2C domain containing) rn
RAE230A:1376392_AT Protein phosphatase 1F (PP2C domain containing) rn
RAT230_2:1376392_AT Protein phosphatase 1F (PP2C domain containing) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6470 protein amino acid dephosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
8287 protein serine/threonine phosphatase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
4722 protein serine/threonine phosphatase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC46598 Feminization of xx and xo animals protein 2 [Caenorhabditis elegans] ref|NP_497224.1| FEMinization of XX and XO animals family member (fem-2) [Caenorhabditis elegans] sp|P49594|FEM2_CAEEL Ca(2+)/calmodulin-dependent protein kinase phosphatase (CaM-kinase phosphatase) (CaMKPase) (Sex-determining protein fem-2) (Feminization of XX and XO animals protein 2) gb|AAC06328.1| putative protein phosphatase [Caenorhabditis elegans] 0.0
blast AAM33404 putative protein phosphatase FEM-2 [Caenorhabditis remanei] 1.0E-154
blast AAF04557 putative PP2C protein phosphatase FEM-2 [Caenorhabditis sp. CB5161] 1.0E-151
Protein Domains
Database ID Description E-Value
Pfam IPR001932 EMBL-EBI Protein phosphatase 2C-like 3.8E-87
Sequence
Target Sequence 
>C. ELEGANS:188540_AT
agaagcttatggcgctggcgtggctcggtgactcacccggatacgtcatgtcgaacattg
aattccgtcagttgacacgtggacactcgccatccgatgaacgggaagctcggcgtgttg
aggaagctggcggccagctttttgtgattggaggcgaattgcgggtgaacggagttctaa
atctcacccgagcactcggagatgttcctggacggccgatgatctctaatgaaccggaaa
cgtgtcaggtgcctattgaaagctcggattatctggtgctcctggcttgtgatggaattt
ctgatgttttcaacgagcgtgacctgtaccagttggtggaggcatttgcgaatgattatc
ctgttgaagactacgcggagctctcccgtttcatctgtacaaaagcgattgaggctggaa
gtgccgacaacgtgtctgttgtgattggctttttgcgtccacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGCTTATGGCGCTGGCGTGGCT 387 73 830 Antisense
CTCACCCGGATACGTCATGTCGAAC 81 229 861 Antisense
GTCAGTTGACACGTGGACACTCGCC 645 465 896 Antisense
CGGAGTTCTAAATCTCACCCGAGCA 685 241 999 Antisense
CCGAGCACTCGGAGATGTTCCTGGA 686 263 1017 Antisense
GACGGCCGATGATCTCTAATGAACC 182 377 1040 Antisense
CCGGAAACGTGTCAGGTGCCTATTG 418 229 1063 Antisense
AAGCTCGGATTATCTGGTGCTCCTG 1 151 1089 Antisense
TTCAACGAGCGTGACCTGTACCAGT 496 677 1138 Antisense
CTCTCCCGTTTCATCTGTACAAAAG 589 231 1210 Antisense
TTGTGATTGGCTTTTTGCGTCCACC 49 707 1268 Antisense
Sequence Source Affymetrix Proprietary Database