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[188538_at]

Description ZK1251.2 /REP_DB=WormBase Gene ID /WP=CE03849 /GEN=ins-7 /TR=SW:Q23430 /GB=CAA92498.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 47      6grp_total > 95      4grp_total > 41      6grp_cgc > 78      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S5      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 91
1 2 3 4 range
A 47.1
(-3)
P 		138.5
(3)
P 		107.6
(1)
P 		67
(-1.7)
P 		91
B 52.8
(-3)
P 		90.4
(0.3)
P 		120.7
(3)
P 		77.7
(-0.8)
M 		68
C 49.9
(-3)
P 		95
(3)
P 		93
(2.7)
P 		84.9
(1.7)
P 		45
avr 49.9
(-3) 		108
(3) 		107.1
(2.9) 		76.5
(-0.3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 240
1 2 3 4 5 6 range
A 58.4
(-1.7)
P 		13.2
(-3)
A 		57.5
(-1.7)
A 		89.5
(-0.8)
P 		216.9
(3)
P 		173.5
(1.7)
P 		204
B 43.9
(-2.7)
P 		34.3
(-3)
A 		41.1
(-2.8)
M 		80.9
(-1.7)
P 		193.6
(1.4)
P 		252.8
(3)
P 		219
C 27.8
(-3)
P 		33.4
(-2.8)
A 		33.7
(-2.8)
P 		110.2
(-0.3)
P 		207.8
(3)
P 		188.5
(2.4)
P 		180
avr 43.4
(-2.5) 		27
(-3) 		44.1
(-2.4) 		93.5
(-0.8) 		206.1
(3) 		204.9
(3) 		179
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 17.2
(-0.8)
P 		30.1
(3)
P 		28.1
(2.4)
P 		9.8
(-3)
P 		20
B 16.9
(-2.8)
P 		22.3
(0)
P 		28
(3)
P 		16.5
(-3)
M 		12
C 16.5
(-2.3)
P 		27.3
(1.3)
P 		32.4
(3)
P 		14.3
(-3)
P 		18
avr 16.9
(-1.7) 		26.6
(1.9) 		29.5
(3) 		13.5
(-3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 10.8
(-2.4)
P 		4.7
(-3)
A 		12.5
(-2.2)
A 		22.7
(-1.2)
P 		65.5
(3)
P 		42.3
(0.7)
P 		61
B 15.3
(-2.6)
P 		11.4
(-2.9)
A 		10
(-3)
M 		21.4
(-2.2)
P 		78.7
(1.9)
P 		94.3
(3)
P 		84
C 10.3
(-2.8)
P 		10.2
(-2.8)
A 		8.6
(-3)
P 		22.2
(-1.6)
P 		67.2
(3)
P 		63.1
(2.6)
P 		59
avr 12.1
(-2.7) 		8.8
(-3) 		10.4
(-2.8) 		22.1
(-1.7) 		70.5
(3) 		66.6
(2.6) 		62
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188538_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8684
Sequence Type Exemplar sequence
Representative Public ID ZK1251.2 NCBI
Target Description ZK1251.2 /REP_DB=WormBase Gene ID /WP=CE03849 /GEN=ins-7 /TR=SW:Q23430 /GB=CAA92498.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069316(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069316 NCBI Caenorhabditis elegans INSulin related family member (ins-7) (ins-7) mRNA, complete cds. 11/11 None
GENEFINDER00000023399 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9682040:9682398:-1 11/11 None
ZK1251.2 ENSEMBL cdna:known chromosome:CEL140:IV:9682040:9682398:-1 gene:ZK1251.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9682045-9682404(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-7
Ensembl ZK1251.2
Entrez Gene 191688 Entrez gene
SwissProt Q23430 EMBL-EBI
WormBase CE03849 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA92498 Hypothetical protein ZK1251.2 [Caenorhabditis elegans] ref|NP_501717.1| INSulin related family member (ins-7) [Caenorhabditis elegans] sp|Q23430|ILB4_CAEEL Probable insulin-like peptide beta-type 4 precursor 1.0E-56
blast CAE18046 Hypothetical protein ZK1251.11 [Caenorhabditis elegans] ref|NP_001023609.1| INSulin related family member (ins-8) [Caenorhabditis elegans] 2.0E-31
blast AAC46744 Insulin related protein 4 [Caenorhabditis elegans] ref|NP_495196.1| INSulin related family member (ins-4) [Caenorhabditis elegans] sp|Q09626|ILB1_CAEEL Probable insulin-like peptide beta-type 1 precursor 7.0E-12
Protein Domains
Database ID Description E-Value
Pfam IPR003235 EMBL-EBI Nematode insulin-related peptide, beta type 5.9E-38
Sequence
Target Sequence 
>C. ELEGANS:188538_AT
ttttggttttaatccctgcttctcaacaatatcctttttcactggagtccttaaatgatc
aaataatcaatgaagaagtaatcgaatatatgcttgaaaattcaattaggtccagcagaa
ccagaagagtccctgacgagaaaaaaatttatcgttgtggaagaagaatacattcgtatg
tgtttgcggtttgtggaaaagcatgcgaatcgaatactgaagttaatattgcatcaaaat
gttgccgtgaagaatgcaccgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTTGGTTTTAATCCCTGCTTCTCA 95 677 38 Antisense
TAATCCCTGCTTCTCAACAATATCC 687 635 47 Antisense
GCTTCTCAACAATATCCTTTTTCAC 10 307 55 Antisense
TATCCTTTTTCACTGGAGTCCTTAA 517 649 67 Antisense
TGAAAATTCAATTAGGTCCAGCAGA 167 573 132 Antisense
GTCCAGCAGAACCAGAAGAGTCCCT 329 477 147 Antisense
GAACCAGAAGAGTCCCTGACGAGAA 125 345 155 Antisense
CATTCGTATGTGTTTGCGGTTTGTG 307 215 208 Antisense
GCATGCGAATCGAATACTGAAGTTA 614 309 238 Antisense
ATATTGCATCAAAATGTTGCCGTGA 186 19 263 Antisense
ATGTTGCCGTGAAGAATGCACCGAC 395 47 276 Antisense
Sequence Source Affymetrix Proprietary Database