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[188532_s_at] helicase

Description T05E8.3 /REP_DB=WormBase Gene ID /WP=CE13245 /TR=O01598 /GB=AAB52427.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=helicase
Cluster 6grp_highest_10_10 > 86      6grp_high1_10_10 > 77      4grp_highest_8_8 > 42      4grp_high_8_8 > 41      6grp_highest_nonorm > 91      6grp_total > 78      4grp_total > 54      6grp_highest_sub > Da      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 207
1 2 3 4 range
A 394.9
(3)
P 		242.4
(-1.4)
P 		293.2
(0)
P 		188.2
(-3)
P 		207
B 375.8
(3)
P 		218.5
(-2.3)
P 		277.3
(-0.3)
P 		196.7
(-3)
P 		179
C 378.6
(3)
P 		285.7
(-0.2)
P 		224.1
(-2.3)
P 		203.6
(-3)
P 		175
avr 383.1
(3) 		248.9
(-1.3) 		264.9
(-0.8) 		196.2
(-3) 		187

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 264
1 2 3 4 5 6 range
A 214.7
(0.4)
P 		219.3
(0.6)
P 		100.5
(-3)
P 		156.5
(-1.3)
P 		235.6
(1.1)
P 		300.1
(3)
P 		200
B 130.4
(-3)
P 		154.6
(-2.4)
P 		170
(-2)
P 		144.7
(-2.6)
P 		193.6
(-1.4)
P 		362
(3)
P 		232
C 217.8
(-1.7)
P 		182
(-2.9)
P 		254.2
(-0.6)
P 		178.2
(-3)
P 		185.3
(-2.8)
P 		364.4
(3)
P 		186
avr 187.6
(-2.1) 		185.3
(-2.2) 		174.9
(-2.5) 		159.8
(-3) 		204.8
(-1.5) 		342.2
(3) 		182
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 143.8
(3)
P 		52.6
(-1.7)
P 		76.7
(-0.5)
P 		27.6
(-3)
P 		116
B 119.8
(3)
P 		54
(-2.1)
P 		64.3
(-1.3)
P 		41.9
(-3)
P 		78
C 125.3
(3)
P 		82.1
(0.2)
P 		78.1
(-0.1)
P 		34.3
(-3)
P 		91
avr 129.6
(3) 		62.9
(-1.2) 		73
(-0.6) 		34.6
(-3) 		95

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 113
1 2 3 4 5 6 range
A 39.6
(-1.1)
P 		78
(3)
P 		21.9
(-3)
P 		39.7
(-1.1)
P 		71.1
(2.3)
P 		73.1
(2.5)
P 		56
B 45.4
(-2.6)
P 		51.5
(-2.2)
P 		41.3
(-2.8)
P 		38.3
(-3)
P 		78.7
(-0.5)
P 		135
(3)
P 		97
C 80.7
(0.1)
P 		55.5
(-1.6)
P 		64.7
(-1)
P 		35.8
(-3)
P 		60
(-1.3)
P 		122
(3)
P 		86
avr 55.2
(-1.6) 		61.7
(-1) 		42.6
(-2.6) 		37.9
(-3) 		69.9
(-0.3) 		110
(3) 		72
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188532_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.602
Sequence Type Exemplar sequence
Representative Public ID T05E8.3 NCBI
Target Description T05E8.3 /REP_DB=WormBase Gene ID /WP=CE13245 /TR=O01598 /GB=AAB52427.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059203(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059203 NCBI Caenorhabditis elegans T05E8.3 (helicase) mRNA, complete cds. 11/11 None
SNAP00000033229 ENSEMBL cdna:SNAP chromosome:CEL140:I:5472640:5478679:-1 11/11 None
T05E8.3 ENSEMBL cdna:known chromosome:CEL140:I:5472324:5479441:-1 gene:T05E8.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5472639-5479441(-) 98.79 100.0
Public Domain and Genome References
Gene Title helicase
Ensembl T05E8.3
Entrez Gene 172199 Entrez gene
SwissProt O01598 EMBL-EBI
WormBase CE37043 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52427 Hypothetical protein T05E8.3 [Caenorhabditis elegans] ref|NP_491604.3| T05E8.3 [Caenorhabditis elegans] 0.0
blast CAE67071 Hypothetical protein CBG12480 [Caenorhabditis briggsae] 0.0
blast AAH74605 DHX33 protein [Xenopus tropicalis] 1.0E-124
Protein Domains
Database ID Description E-Value
Pfam IPR007502 EMBL-EBI Helicase-associated region 3.5E-28
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 3.7E-14
Pfam IPR007502 EMBL-EBI Helicase-associated region 3.5E-28
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 3.7E-14
Sequence
Target Sequence 
>C. ELEGANS:188532_S_AT
gtgatcatatcacgctactcaagcttgttttagttactaatcagttttttgatcagttga
acaagaaaacagaatctgatgcgagatacgggaaggccaataaatcattggaacgtgaat
ataatcaaactatcaggaaattctgcaatgataatatgatcaacgaacaacatttgaaaa
cagcttcaatgattgaggaacaattaaaacaaattgcaattgaacagaaagttacattct
caacgtgtggagctgattttatgaaaatgagaaaagcgttggctctcggaatgttcttga
atagttgtgaatatgatcgtcaggaagatcgatatcgattaatgattaatcctgcgatta
ctctgaaaattcatccatcgtcttgcctctcaagatcaaaacctgcatacattgtgtttt
ctgaattaatgaagactaatgatctctttgcgctacaagtgacccttatcgacggcgatt
gggttcgcccattgatcagcgatcacaagaaaatgcgtgcaaatcatttggctgaaacga
cagaacgagtacaacaaatcactcgtttagaacca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGATCATATCACGCTACTCAAGCT 87 485 2462 Antisense
GCTACTCAAGCTTGTTTTAGTTACT 161 303 2475 Antisense
AAAGCGTTGGCTCTCGGAATGTTCT 678 123 2734 Antisense
AATCCTGCGATTACTCTGAAAATTC 58 169 2809 Antisense
TGAAAATTCATCCATCGTCTTGCCT 461 573 2825 Antisense
CTTGCCTCTCAAGATCAAAACCTGC 146 219 2843 Antisense
AACCTGCATACATTGTGTTTTCTGA 166 145 2861 Antisense
AAGTGACCCTTATCGACGGCGATTG 641 157 2918 Antisense
ATCGACGGCGATTGGGTTCGCCCAT 698 33 2929 Antisense
GGTTCGCCCATTGATCAGCGATCAC 592 507 2943 Antisense
ACAACAAATCACTCGTTTAGAACCA 80 111 3012 Antisense
Sequence Source Affymetrix Proprietary Database