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[188531_s_at]

Description C04A2.3 /REP_DB=WormBase Gene ID /WP=CE27421 /GEN=egl-27 /TR=SW:Q09229 /GB=AAC46733.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 09      6grp_total > 37      4grp_total > 31      6grp_cgc > 16      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 128
1 2 3 4 range
A 85
(0.4)
P 		39.8
(-3)
A 		62.8
(-1.3)
P 		119.8
(3)
P 		80
B 100.5
(3)
P 		72
(-0.7)
P 		54.6
(-3)
P 		98.1
(2.7)
P 		46
C 167.9
(3)
P 		69.2
(-2.7)
A 		64.5
(-3)
P 		67
(-2.9)
A 		103
avr 117.8
(3) 		60.3
(-3) 		60.6
(-3) 		95
(0.6) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 40.9
(-0.4)
A 		77.9
(3)
P 		36.4
(-0.8)
A 		40.7
(-0.4)
P 		12.5
(-3)
A 		63.3
(1.7)
P 		65
B 55.2
(2.8)
P 		41.1
(1.2)
P 		4.3
(-3)
A 		34.3
(0.4)
A 		39.4
(1)
P 		57.4
(3)
P 		53
C 90.5
(3)
P 		39.5
(-0.8)
P 		47.9
(-0.2)
A 		10.7
(-3)
A 		44.9
(-0.4)
A 		52.1
(0.1)
P 		80
avr 62.2
(3) 		52.8
(1.3) 		29.5
(-2.8) 		28.6
(-3) 		32.3
(-2.3) 		57.6
(2.2) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 31
(3)
P 		8.6
(-3)
A 		16.4
(-0.9)
P 		17.5
(-0.6)
P 		22
B 32.1
(3)
P 		17.8
(-1.4)
P 		12.6
(-3)
P 		20.9
(-0.4)
P 		20
C 55.6
(3)
P 		19.9
(-1.8)
A 		22.5
(-1.5)
P 		11.3
(-3)
A 		44
avr 39.6
(3) 		15.4
(-3) 		17.2
(-2.6) 		16.6
(-2.7) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 7.5
(-2.1)
A 		27.7
(3)
P 		7.9
(-2)
A 		10.3
(-1.4)
P 		3.8
(-3)
A 		15.4
(-0.1)
P 		24
B 19.2
(2.4)
P 		13.7
(0.7)
P 		1
(-3)
A 		9.1
(-0.6)
A 		16
(1.4)
P 		21.4
(3)
P 		20
C 33.5
(3)
P 		12
(-1.1)
P 		12.2
(-1.1)
A 		2.1
(-3)
A 		14.5
(-0.6)
A 		17.5
(-0.1)
P 		31
avr 20.1
(3) 		17.8
(1.9) 		7
(-3) 		7.2
(-2.9) 		11.4
(-1) 		18.1
(2.1) 		13
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188531_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5649
Sequence Type Exemplar sequence
Representative Public ID C04A2.3 NCBI
Target Description C04A2.3 /REP_DB=WormBase Gene ID /WP=CE27421 /GEN=egl-27 /TR=SW:Q09229 /GB=AAC46733.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171011(11), NM_001026740(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171011 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. 11/11 A 
NM_001026740 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. 11/11 None
GENEFINDER00000035668 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6835402:6847242:1 10/11 None
C04A2.3a ENSEMBL cdna:known chromosome:CEL140:II:6834951:6847836:1 gene:C04A2.3 11/11 A 
C04A2.3b ENSEMBL cdna:known chromosome:CEL140:II:6835011:6847242:1 gene:C04A2.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
174121 3 2 NM_001026740 188531_s_at (11)
NM_171011 175679_at (11), 188531_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6834597-6836800(+) 100.0 100.0
Public Domain and Genome References
Ensembl C04A2.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM81126 Egg laying defective protein 27, isoform a [Caenorhabditis elegans] ref|NP_741012.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] sp|Q09228|EGL27_CAEEL Egg laying defective protein 0.0
blast AAD27790 EGL-27 [Caenorhabditis elegans] 0.0
blast AAM81127 Egg laying defective protein 27, isoform b [Caenorhabditis elegans] ref|NP_001021911.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000949 EMBL-EBI ELM2 domain 8.5E-18
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000949 EMBL-EBI ELM2 domain 8.5E-18
Sequence
Target Sequence 
>C. ELEGANS:188531_S_AT
gtttcttccccaatggaaaacgatgacgaaccagaattttcccaaaagcattacgatatt
gaaccatgttattatagtcttacaggcaaatcggataggaattgccgtggaatagtatac
cggtatcgacaagacagtgatctaaaagggtttcaatcacatgatggcacattgtacagg
ctacgagattcagtattcgtagaagtgtcacaaaacgagccgtatgtgatagcggcaata
tgcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTCTTCCCCAATGGAAAACGATG 669 445 208 Antisense
GATGACGAACCAGAATTTTCCCAAA 489 403 229 Antisense
GAACCATGTTATTATAGTCTTACAG 703 345 268 Antisense
AGTCTTACAGGCAAATCGGATAGGA 153 59 283 Antisense
GGAATTGCCGTGGAATAGTATACCG 640 531 305 Antisense
AGTATACCGGTATCGACAAGACAGT 667 57 321 Antisense
GGGTTTCAATCACATGATGGCACAT 342 497 355 Antisense
GATGGCACATTGTACAGGCTACGAG 31 407 370 Antisense
GTACAGGCTACGAGATTCAGTATTC 610 459 381 Antisense
GAAGTGTCACAAAACGAGCCGTATG 41 335 409 Antisense
GTATGTGATAGCGGCAATATGCGGA 74 455 429 Antisense
Sequence Source Affymetrix Proprietary Database