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[188530_at]

Description C04A2.3 /REP_DB=WormBase Gene ID /WP=CE27421 /GEN=egl-27 /TR=SW:Q09229 /GB=AAC46733.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_total > 82      4grp_total > 0      6grp_cgc > 74      6grp_cgc_sub > 02_03_04_05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 50
1 2 3 4 range
A 24.6
(-0.2)
A 		4.8
(-2.9)
A 		3.9
(-3)
A 		48
(3)
A 		44
B 6
(-2.6)
A 		3.8
(-3)
A 		38.6
(3)
A 		8.9
(-2.1)
A 		35
C 6.5
(-3)
A 		17.8
(-1.6)
A 		13.1
(-2.2)
A 		53.7
(3)
A 		47
avr 12.4
(-2.2) 		8.8
(-3) 		18.5
(-0.9) 		36.9
(3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 10.3
(-0.7)
A 		16.5
(2.3)
A 		12.5
(0.4)
A 		18.1
(3)
A 		17.9
(2.9)
A 		5.3
(-3)
A 		13
B 4.8
(-2.5)
A 		17
(1.4)
A 		16.6
(1.3)
A 		21.9
(3)
A 		3.2
(-3)
A 		3.1
(-3)
A 		19
C 15.4
(-0.3)
A 		13.1
(-0.9)
A 		6.9
(-2.4)
A 		4.4
(-3)
A 		29.1
(3)
A 		4.5
(-3)
A 		25
avr 10.2
(-0.1) 		15.5
(2.4) 		12
(0.7) 		14.8
(2.1) 		16.7
(3) 		4.3
(-3) 		12
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 8
1 2 3 4 range
A 9
(3)
A 		1
(-3)
A 		1
(-3)
A 		7
(1.5)
A 		8
B 1.9
(-2.3)
A 		0.9
(-3)
A 		8.9
(3)
A 		1.9
(-2.3)
A 		8
C 2.1
(-3)
A 		5.1
(-0.4)
A 		4.6
(-0.9)
A 		9.1
(3)
A 		7
avr 4.3
(0.2) 		2.3
(-3) 		4.8
(1.1) 		6
(3) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9
1 2 3 4 5 6 range
A 1.9
(-2.2)
A 		5.9
(3)
A 		2.7
(-1.2)
A 		4.6
(1.3)
A 		5.4
(2.3)
A 		1.3
(-3)
A 		5
B 1.7
(-2.2)
A 		5.7
(2.9)
A 		4
(0.7)
A 		5.8
(3)
A 		1.3
(-2.7)
A 		1.1
(-3)
A 		5
C 5.7
(0.4)
A 		4
(-0.8)
A 		1.8
(-2.4)
A 		0.9
(-3)
A 		9.4
(3)
A 		1.5
(-2.6)
A 		9
avr 3.1
(-0.4) 		5.2
(2.7) 		2.8
(-0.8) 		3.8
(0.7) 		5.4
(3) 		1.3
(-3) 		4
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188530_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5649
Sequence Type Exemplar sequence
Representative Public ID C04A2.3 NCBI
Target Description C04A2.3 /REP_DB=WormBase Gene ID /WP=CE27421 /GEN=egl-27 /TR=SW:Q09229 /GB=AAC46733.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026740, NM_171011
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000035668 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6835402:6847242:1 None
NM_171011 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. A 
C04A2.3a ENSEMBL cdna:known chromosome:CEL140:II:6834951:6847836:1 gene:C04A2.3 A 
NM_001026740 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-27) (egl-27) mRNA, complete cds. None
C04A2.3b ENSEMBL cdna:known chromosome:CEL140:II:6835011:6847242:1 gene:C04A2.3 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000035661 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6834597-6836800(+) 100.0 100.0
Public Domain and Genome References
Ensembl C04A2.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM81126 Egg laying defective protein 27, isoform a [Caenorhabditis elegans] ref|NP_741012.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] sp|Q09228|EGL27_CAEEL Egg laying defective protein 0.0
blast AAD27790 EGL-27 [Caenorhabditis elegans] 0.0
blast AAM81127 Egg laying defective protein 27, isoform b [Caenorhabditis elegans] ref|NP_001021911.1| EGg Laying defective family member (egl-27) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000949 EMBL-EBI ELM2 domain 8.5E-18
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000679 EMBL-EBI Zn-finger, GATA type 1.2E-8
Pfam IPR001005 EMBL-EBI Myb DNA-binding domain 1.6E-9
Pfam IPR000949 EMBL-EBI ELM2 domain 8.5E-18
Sequence
Target Sequence 
>C. ELEGANS:188530_AT
caatgtctcttttcctaacggaccagtcgtgtatgtgctctgtgtatgtgccccactaac
cgacgacgacgaccctcgcccgccccacgccactcccacaccccaccccgtcttcatccc
tttcctctctcccgtcttcctttcttctatccctcggtgctgttctaacggcgtttcttc
cccaatggaaaacgatgacgaaccagaattttcccaaaagcattacgatattgaaccatg
ttattatagtcttacaggcaaatcggataggaattgccgtggaatagtataccggtatcg
acaagacagtgatctaaaagggtttcaatcacatgatggcacattgtacaggctacgaga
ttcagtattcgtagaagtgtcacaaaacgagccgtatgtgatagcggcaatatgcggatt
taaatatgtgagtaatgagatcgtgaggcatgtcagcagatggttctgccatattaaggt
ttttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAATGTCTCTTTTCCTAACGGACCA 463 181 36 Antisense
TCTCTTTTCCTAACGGACCAGTCGT 5 607 41 Antisense
AACGGACCAGTCGTGTATGTGCTCT 537 143 52 Antisense
GGACCAGTCGTGTATGTGCTCTGTG 386 523 55 Antisense
AGTCGTGTATGTGCTCTGTGTATGT 379 61 60 Antisense
TGTATGTGCTCTGTGTATGTGCCCC 312 561 65 Antisense
ATGTGAGTAATGAGATCGTGAGGCA 286 49 461 Antisense
GAGATCGTGAGGCATGTCAGCAGAT 589 395 472 Antisense
GTGAGGCATGTCAGCAGATGGTTCT 684 483 478 Antisense
GCATGTCAGCAGATGGTTCTGCCAT 352 311 483 Antisense
GGTTCTGCCATATTAAGGTTTTTGA 346 507 497 Antisense
Sequence Source Affymetrix Proprietary Database