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[188529_at]

Description C24H12.4 /REP_DB=WormBase Gene ID /WP=CE27728 /TR=O17157 /GB=AAB71200.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=helicase
Cluster 6grp_highest_10_10 > 85      6grp_high1_10_10 > 68      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 85      6grp_total > 58      4grp_total > 03      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2588
1 2 3 4 range
A 1009.1
(-2)
P 		782.6
(-2.5)
P 		582.3
(-3)
P 		3051.3
(3)
P 		2469
B 935.6
(-2.3)
P 		975.1
(-2.2)
P 		644
(-3)
P 		3135.7
(3)
P 		2492
C 966.3
(-1.9)
P 		548.1
(-3)
P 		636.9
(-2.8)
P 		2750.1
(3)
P 		2202
avr 970.3
(-2.1) 		768.6
(-2.6) 		621.1
(-3) 		2979
(3) 		2358

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 464
1 2 3 4 5 6 range
A 932.4
(2.6)
P 		675.2
(-1.4)
P 		566.8
(-3)
P 		603
(-2.4)
P 		704.9
(-0.9)
P 		961.5
(3)
P 		395
B 816.6
(-0.4)
P 		814.7
(-0.4)
P 		650.8
(-3)
P 		709.2
(-2.1)
P 		792.9
(-0.8)
P 		1030.3
(3)
P 		380
C 640.4
(-2.2)
P 		815.7
(0.4)
P 		715.9
(-1.1)
P 		740
(-0.7)
P 		588.1
(-3)
P 		989
(3)
P 		401
avr 796.5
(-0.4) 		768.5
(-0.9) 		644.5
(-3) 		684.1
(-2.3) 		695.3
(-2.1) 		993.6
(3) 		349
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 519
1 2 3 4 range
A 367.4
(1.4)
P 		169.9
(-2.6)
P 		152.2
(-3)
P 		446.7
(3)
P 		295
B 298.3
(-1.3)
P 		240.8
(-1.9)
P 		149.2
(-3)
P 		668.2
(3)
P 		519
C 319.9
(0.2)
P 		157.6
(-3)
P 		222
(-1.7)
P 		463.7
(3)
P 		306
avr 328.5
(-0.4) 		189.4
(-2.7) 		174.5
(-3) 		526.2
(3) 		352

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 261
1 2 3 4 5 6 range
A 171.9
(-0.5)
P 		240.1
(3)
P 		123.4
(-3)
P 		152.9
(-1.5)
P 		212.7
(1.6)
P 		234.1
(2.7)
P 		117
B 284.1
(0.3)
P 		271.2
(0)
P 		158
(-3)
P 		187.8
(-2.2)
P 		322.1
(1.4)
P 		384.2
(3)
P 		226
C 237.4
(-0.1)
P 		248.9
(0.3)
P 		182.2
(-1.9)
P 		148.8
(-3)
P 		190.3
(-1.6)
P 		331.1
(3)
P 		182
avr 231.1
(-0.2) 		253.4
(0.7) 		154.5
(-3) 		163.2
(-2.7) 		241.7
(0.2) 		316.5
(3) 		162
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188529_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5503
Sequence Type Exemplar sequence
Representative Public ID C24H12.4 NCBI
Target Description C24H12.4 /REP_DB=WormBase Gene ID /WP=CE27728 /TR=O17157 /GB=AAB71200.2 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170970(11), NM_171847(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170970 NCBI Caenorhabditis elegans C24H12.4a (helicase) mRNA, complete cds. 11/11 None
NM_171847 NCBI Caenorhabditis elegans C24H12.4b (helicase) mRNA, complete cds. 11/11 None
SNAP00000023826 ENSEMBL cdna:SNAP chromosome:CEL140:II:411231:416528:1 11/11 None
GENEFINDER00000023835 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:411231:416528:1 11/11 None
C24H12.4b ENSEMBL cdna:known chromosome:CEL140:II:411221:416606:1 gene:C24H12.4 11/11 None
C24H12.4a ENSEMBL cdna:known chromosome:CEL140:II:411221:416528:1 gene:C24H12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:411230-416528(+) 100.0 100.0
Public Domain and Genome References
Ensembl C24H12.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB71200 Hypothetical protein C24H12.4a [Caenorhabditis elegans] ref|NP_740966.1| C24H12.4a [Caenorhabditis elegans] 0.0
blast CAE62876 Hypothetical protein CBG07062 [Caenorhabditis briggsae] 0.0
blast AAL16306 Hypothetical protein C24H12.4b [Caenorhabditis elegans] ref|NP_740967.1| C24H12.4b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.6E-11
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.7E-65
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 2.6E-11
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.4E-40
Sequence
Target Sequence 
>C. ELEGANS:188529_AT
ggggaatcgattttcatcacgtttcgaatgttgttaatttcgattttcccgaaactacag
atgcttatattcatcgtgttggaagaaccgcccgtggtttcaacaaaggaaccgcccttt
ccttctgtattccatcggaacgtgcacatttggagcaaattcaggaggaaattaatcagc
aaatgggtcgaaaagtgcttcaaccatacgaattccgtattaaagagctcgacactttcc
tcctgagaacccgtgaagctttgagtaaatgcacaaaaggagttatcaagaaggctcggc
tgaaggagattcgtcaggagctcatgcgctcggccaacctccaaacgtttttcgcgaaaa
acgaacgtgagaagcttctgatgcagacggattgtcaccctgtcatgctgaaaatcaact
ctcccgcaatcgccgatgtcacatcctacatggtaccagaagcccttcgtggaatggatt
tctcggctcccggagccaaaaatcgccgctacaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGGAATCGATTTTCATCACGTTTC 330 497 1379 Antisense
CCTTTCCTTCTGTATTCCATCGGAA 395 269 1494 Antisense
AGTGCTTCAACCATACGAATTCCGT 109 61 1572 Antisense
ATTAAAGAGCTCGACACTTTCCTCC 375 13 1597 Antisense
CTCCTGAGAACCCGTGAAGCTTTGA 32 233 1618 Antisense
GAGATTCGTCAGGAGCTCATGCGCT 154 397 1684 Antisense
GATGCAGACGGATTGTCACCCTGTC 468 413 1758 Antisense
GCCGATGTCACATCCTACATGGTAC 335 285 1810 Antisense
CATGGTACCAGAAGCCCTTCGTGGA 577 209 1827 Antisense
TCGTGGAATGGATTTCTCGGCTCCC 158 605 1845 Antisense
CGGAGCCAAAAATCGCCGCTACAAT 397 193 1869 Antisense
Sequence Source Affymetrix Proprietary Database