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[188528_at]

Description H19M22.3 /REP_DB=WormBase Gene ID /WP=CE26937 /TR=O44836 /GB=AAK21412.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=protease
Cluster 6grp_total > 61      4grp_total > 48      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 189
1 2 3 4 range
A 4.1
(-3)
A 		170.8
(3)
A 		122.3
(1.3)
A 		5.8
(-2.9)
A 		167
B 4
(-3)
A 		129.7
(1)
A 		192.8
(3)
A 		20.9
(-2.5)
A 		189
C 4.5
(-3)
A 		77.3
(-0.4)
A 		170.3
(3)
A 		55.5
(-1.2)
A 		166
avr 4.2
(-3) 		125.9
(1.6) 		161.8
(3) 		27.4
(-2.1) 		158

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 25.1
(-1.7)
A 		34.5
(-1.1)
A 		22.4
(-1.9)
A 		101.3
(3)
A 		48.6
(-0.3)
A 		4.3
(-3)
A 		97
B 39.9
(-0.8)
A 		10.1
(-2.6)
A 		103.3
(3)
A 		81.3
(1.7)
A 		22.6
(-1.9)
A 		4.3
(-3)
A 		99
C 109.3
(3)
A 		4.6
(-3)
A 		81.6
(1.4)
A 		55.9
(-0.1)
A 		82
(1.4)
A 		7
(-2.9)
A 		105
avr 58.1
(1.3) 		16.4
(-2.1) 		69.1
(2.2) 		79.5
(3) 		51.1
(0.7) 		5.2
(-3) 		74
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 1.5
(-2.9)
A 		37.1
(3)
A 		32
(2.2)
A 		0.8
(-3)
A 		36
B 1.3
(-3)
A 		32
(1.2)
A 		44.7
(3)
A 		4.5
(-2.6)
A 		43
C 1.5
(-3)
A 		22.2
(-0.9)
A 		59.3
(3)
A 		9.4
(-2.2)
A 		58
avr 1.4
(-3) 		30.4
(1) 		45.3
(3) 		4.9
(-2.5) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 4.6
(-2.1)
A 		12.3
(-0.3)
A 		4.9
(-2.1)
A 		25.7
(3)
A 		14.7
(0.3)
A 		1
(-3)
A 		25
B 13.9
(0.1)
A 		3.4
(-2.5)
A 		25.1
(3)
A 		21.5
(2.1)
A 		9.2
(-1.1)
A 		1.6
(-3)
A 		24
C 40.5
(3)
A 		1.4
(-3)
A 		20.8
(-0)
A 		11.2
(-1.5)
A 		26.5
(0.9)
A 		2.3
(-2.9)
A 		39
avr 19.7
(3) 		5.7
(-1.6) 		16.9
(2.1) 		19.5
(2.9) 		16.8
(2) 		1.6
(-3) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188528_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7352
Sequence Type Exemplar sequence
Representative Public ID H19M22.3 NCBI
Target Description H19M22.3 /REP_DB=WormBase Gene ID /WP=CE26937 /TR=O44836 /GB=AAK21412.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065193(11), NM_065195(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065193 NCBI Caenorhabditis elegans H19M22.3a (protease) mRNA, complete cds. 11/11 None
NM_065195 NCBI Caenorhabditis elegans H19M22.3c (protease) mRNA, complete cds. 10/11 None
GENEFINDER00000033413 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:2638587:2642247:1 11/11 None
H19M22.3a ENSEMBL cdna:known chromosome:CEL140:III:2636394:2642837:1 gene:H19M22.3 11/11 A 
H19M22.3c ENSEMBL cdna:known chromosome:CEL140:III:2638502:2642247:1 gene:H19M22.3 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000033409 8/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175383 3 2 NM_065193 175035_at (11), 188528_at (11)
NM_065195 188528_at (10)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:2637023-2641674(+) 100.0 100.0
Public Domain and Genome References
Ensembl H19M22.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK21412 Hypothetical protein H19M22.3a [Caenorhabditis elegans] ref|NP_497594.1| H19M22.3a [Caenorhabditis elegans] 0.0
blast T32825 hypothetical protein H19M22.3 - Caenorhabditis elegans 0.0
blast AAL06034 Hypothetical protein H19M22.3c [Caenorhabditis elegans] ref|NP_497596.2| H19M22.3c [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001818 EMBL-EBI Peptidase M10A and M12B, matrixin and adamalysin 3.9E-16
Pfam IPR001818 EMBL-EBI Peptidase M10A and M12B, matrixin and adamalysin 3.9E-16
Pfam IPR001818 EMBL-EBI Peptidase M10A and M12B, matrixin and adamalysin 3.9E-16
Sequence
Target Sequence 
>C. ELEGANS:188528_AT
acttttgcctgtacgtctggcttatgagccagatcgtcgaggcacacaacagctcggcac
agaataatcatggagtcggctcgattaccagctcgaggaccaacaagaaatcttttaaaa
gtgagggtttctttctttttcaattaaaatttcctcattccacactcacacacacggatg
acgttgtgatgcgcgaaaaagataaaagatcgtatcgtggggactcaaagattccaaagt
gctcatcgaataactcaagtcaacggacgcttgccgagaagaagctcacactcggcttgc
atttgagtgaagccgatgcaaaacgatacaccgaaatggtgtgcaacttcctcgccggcc
tccacatgtggcgtacaaatcccaaccatcacgcgtcggaatcactcgaaaaagaataca
aaggagtctcacaggagatgggcactttcagcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACTTTTGCCTGTACGTCTGGCTTAT 351 103 917 Antisense
CAGATCGTCGAGGCACACAACAGCT 547 205 946 Antisense
CATGGAGTCGGCTCGATTACCAGCT 516 207 985 Antisense
GATTACCAGCTCGAGGACCAACAAG 436 429 999 Antisense
ATTAAAATTTCCTCATTCCACACTC 274 13 1059 Antisense
CTCAAGTCAACGGACGCTTGCCGAG 434 227 1170 Antisense
GCTCACACTCGGCTTGCATTTGAGT 579 301 1200 Antisense
CCGAAATGGTGTGCAACTTCCTCGC 207 261 1247 Antisense
TGTGGCGTACAAATCCCAACCATCA 401 553 1283 Antisense
CATCACGCGTCGGAATCACTCGAAA 502 213 1303 Antisense
ACAGGAGATGGGCACTTTCAGCGGA 487 107 1347 Antisense
Sequence Source Affymetrix Proprietary Database