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[188524_at] cuticulin 1

Description M28.1 /REP_DB=WormBase Gene ID /WP=CE23881 /TR=Q21573 /GB=CAA90131.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cuticulin 1
Cluster 6grp_total > 23      4grp_total > 20      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 26.1
(-3)
A 		31
(-2.5)
A 		26.6
(-2.9)
A 		84
(3)
A 		58
B 45.4
(3)
A 		42.4
(2.4)
M 		25.4
(-0.9)
A 		14.8
(-3)
A 		31
C 30.4
(-1.1)
A 		34.4
(-0.6)
A 		11
(-3)
A 		70.7
(3)
A 		60
avr 34
(-0.8) 		35.9
(-0.5) 		21
(-3) 		56.5
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 57.7
(3)
A 		21.4
(-1.4)
A 		45.1
(1.5)
P 		21.5
(-1.4)
A 		20.2
(-1.6)
A 		8.3
(-3)
A 		49
B 28.8
(0.9)
A 		38.5
(3)
A 		11.6
(-2.8)
A 		25.9
(0.3)
A 		10.7
(-3)
A 		37.7
(2.8)
A 		28
C 19.4
(-1.2)
A 		53.7
(3)
M 		39.3
(1.2)
A 		34.5
(0.6)
A 		21.8
(-0.9)
A 		5.2
(-3)
A 		49
avr 35.3
(2.3) 		37.9
(3) 		32
(1.3) 		27.3
(-0.1) 		17.6
(-2.9) 		17.1
(-3) 		21
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 9.5
(-0)
A 		6.7
(-3)
A 		7
(-2.7)
A 		12.3
(3)
A 		6
B 14.5
(3)
A 		10.5
(0.9)
M 		5.9
(-1.6)
A 		3.2
(-3)
A 		11
C 10.1
(1.7)
A 		9.9
(1.5)
A 		3.8
(-3)
A 		11.9
(3)
A 		8
avr 11.4
(3) 		9
(0.5) 		5.6
(-3) 		9.1
(0.6) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 15
1 2 3 4 5 6 range
A 10.6
(3)
A 		7.6
(0.9)
A 		9.8
(2.4)
P 		5.5
(-0.6)
A 		6.1
(-0.1)
A 		2
(-3)
A 		9
B 10
(0.8)
A 		12.8
(2.3)
A 		2.8
(-3)
A 		6.9
(-0.8)
A 		4.3
(-2.2)
A 		14.1
(3)
A 		11
C 7.2
(-0.8)
A 		16.4
(3)
M 		10
(0.4)
A 		6.9
(-0.9)
A 		7
(-0.8)
A 		1.7
(-3)
A 		15
avr 9.3
(0.2) 		12.3
(3) 		7.5
(-1.4) 		6.4
(-2.4) 		5.8
(-3) 		5.9
(-2.9) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188524_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3092
Sequence Type Exemplar sequence
Representative Public ID M28.1 NCBI
Target Description M28.1 /REP_DB=WormBase Gene ID /WP=CE23881 /TR=Q21573 /GB=CAA90131.2 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cuticulin 1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063892(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063892 NCBI Caenorhabditis elegans M28.1 (M28.1) mRNA, complete cds. 11/11 A 
M28.1 ENSEMBL cdna:known chromosome:CEL140:II:10622192:10626345:1 gene:M28.1 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000007399 4/11 Cross Hyb Matching Probes None
GENEFINDER00000007414 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:10622212-10626342(+) 100.0 100.0
Public Domain and Genome References
Gene Title cuticulin 1
Ensembl M28.1
Entrez Gene 174640 Entrez gene
SwissProt Q21573 EMBL-EBI
WormBase CE23881 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA90131 Hypothetical protein M28.1 [Caenorhabditis elegans] ref|NP_496293.1| M28.1 [Caenorhabditis elegans] 0.0
blast CAE59642 Hypothetical protein CBG03056 [Caenorhabditis briggsae] 0.0
blast CAA91480 Hypothetical protein T04F8.4 [Caenorhabditis elegans] ref|NP_509946.1| T04F8.4 [Caenorhabditis elegans] 2.0E-40
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.6E-31
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496293.1 1 524-546
M28.1 1 524-546
Sequence
Target Sequence 
>C. ELEGANS:188524_AT
gttctgtggatgatgctgagtgtctcgatatgacgcctccaaaatgcggcgcgaacaata
atttgctacgaagaaaaaagagatcatcacacggattgtcttcaatgtctctacatgcaa
atagtcttactgtatttgacattgattcatcaaaaactgatacaaatcaactactaccaa
cacctgcattattacggaaaacatttgaagaatacgagagaactttctgtgtttctgttg
caagttttggaatacttatatctgcttcaacgttctttgcaactatttcaattgctgtac
agttcctgttagtcagccgttttttttgtgtacaaactgaaaagttggctgaaaaatcaa
caaacacacccttaagcatgcgatactcatattttgaaattcgaaaaattttgtatttca
aaatcttatcatcacctcctgtttttcaaaatgccgtcacttttgaggtgaccgtttttt
tttgtttgtacttgacaaaaatccaatcaaaacaaaactgcacacagtctgtatttcttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCTGTGGATGATGCTGAGTGTCT 388 443 1337 Antisense
TGAGTGTCTCGATATGACGCCTCCA 708 567 1353 Antisense
GACGCCTCCAAAATGCGGCGCGAAC 473 377 1368 Antisense
GCGGCGCGAACAATAATTTGCTACG 676 293 1382 Antisense
GTACAGTTCCTGTTAGTCAGCCGTT 628 457 1633 Antisense
TAGTCAGCCGTTTTTTTTGTGTACA 191 653 1646 Antisense
AAACACACCCTTAAGCATGCGATAC 349 129 1698 Antisense
TCAAAATCTTATCATCACCTCCTGT 35 631 1754 Antisense
CACCTCCTGTTTTTCAAAATGCCGT 569 201 1769 Antisense
ATGCCGTCACTTTTGAGGTGACCGT 680 35 1787 Antisense
AACTGCACACAGTCTGTATTTCTTT 152 143 1852 Antisense
Sequence Source Affymetrix Proprietary Database