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[188522_at]

Description M04D8.2 /REP_DB=WormBase Gene ID /WP=CE00577 /GEN=ins-22 /TR=SW:Q21508 /GB=CAA83611.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 12      4grp_high_8_8 > 32      6grp_total > 22      4grp_total > 47      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 463
1 2 3 4 range
A 425.8
(3)
P 		99.1
(-2.8)
P 		85.8
(-3)
P 		104.1
(-2.7)
P 		340
B 389.3
(3)
P 		101.7
(-2.7)
A 		87
(-3)
P 		114.3
(-2.5)
P 		302
C 548.7
(3)
P 		134.1
(-2.5)
P 		100
(-3)
P 		98
(-3)
P 		451
avr 454.6
(3) 		111.6
(-2.7) 		90.9
(-3) 		105.5
(-2.8) 		364

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 242
1 2 3 4 5 6 range
A 246.6
(1.8)
P 		242.4
(1.7)
P 		292.5
(3)
P 		118.2
(-1.5)
P 		143.5
(-0.8)
P 		59.3
(-3)
A 		233
B 237
(2)
P 		251
(2.5)
P 		265.5
(3)
P 		119.5
(-2)
P 		123.8
(-1.8)
P 		89.4
(-3)
P 		176
C 228.6
(0.7)
P 		301.7
(3)
P 		168
(-1.1)
P 		233.9
(0.9)
P 		118.8
(-2.7)
P 		108.1
(-3)
P 		194
avr 237.4
(2.1) 		265
(3) 		242
(2.2) 		157.2
(-0.6) 		128.7
(-1.6) 		85.6
(-3) 		179
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 155
(3)
P 		21.5
(-2.7)
P 		22.4
(-2.7)
P 		15.2
(-3)
P 		140
B 124.1
(3)
P 		25.1
(-2.7)
A 		20.2
(-3)
P 		24.4
(-2.8)
P 		104
C 181.6
(3)
P 		38.5
(-2.2)
P 		34.8
(-2.3)
P 		16.5
(-3)
P 		165
avr 153.6
(3) 		28.4
(-2.6) 		25.8
(-2.7) 		18.7
(-3) 		135

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 78
1 2 3 4 5 6 range
A 45.5
(-0.4)
P 		86.2
(3)
P 		63.7
(1.1)
P 		30
(-1.7)
P 		43.3
(-0.6)
P 		14.4
(-3)
A 		72
B 82.5
(2.9)
P 		83.5
(3)
P 		64.5
(0.8)
P 		31.6
(-3)
P 		50.3
(-0.8)
P 		33.3
(-2.8)
P 		52
C 84.7
(2.2)
P 		92.1
(3)
P 		42.8
(-2.3)
P 		47
(-1.8)
P 		38.5
(-2.8)
P 		36.2
(-3)
P 		56
avr 70.9
(1.3) 		87.3
(3) 		57
(-0.1) 		36.2
(-2.2) 		44
(-1.4) 		28
(-3) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188522_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7869
Sequence Type Exemplar sequence
Representative Public ID M04D8.2 NCBI
Target Description M04D8.2 /REP_DB=WormBase Gene ID /WP=CE00577 /GEN=ins-22 /TR=SW:Q21508 /GB=CAA83611.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066822(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066822 NCBI Caenorhabditis elegans INSulin related family member (ins-22) (ins-22) mRNA, complete cds. 11/11 None
SNAP00000022941 ENSEMBL cdna:SNAP chromosome:CEL140:III:10026191:10026502:1 11/11 None
M04D8.2 ENSEMBL cdna:known chromosome:CEL140:III:10026180:10026577:1 gene:M04D8.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000022952 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10026186-10026498(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-22
Ensembl M04D8.2
Entrez Gene 191690 Entrez gene
SwissProt Q21508 EMBL-EBI
WormBase CE00577 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83611 Hypothetical protein M04D8.2 [Caenorhabditis elegans] ref|NP_499223.1| INSulin related family member (ins-22) [Caenorhabditis elegans] sp|Q21508|ILA2_CAEEL Probable insulin-like peptide alpha-type 2 precursor 1.0E-44
blast CAA83609 Hypothetical protein M04D8.3 [Caenorhabditis elegans] ref|NP_499224.1| INSulin related family member (ins-23) [Caenorhabditis elegans] sp|Q21506|ILA3_CAEEL Probable insulin-like peptide alpha-type 3 precursor 9.0E-15
blast CAA83610 Hypothetical protein M04D8.1 [Caenorhabditis elegans] ref|NP_499222.2| INSulin related family member (ins-21) [Caenorhabditis elegans] 1.0E-12
Sequence
Target Sequence 
>C. ELEGANS:188522_AT
tgcacactacaactattctcatatgctttttcatctttcttgttcaagtctccacaatgg
atgctcacactgacaaatacgtcagaactctgtgtggaaaaactgcaatcagaaatattg
ccaacctttgcccgccaaagccagaaatgaagggtatctgttctaccggagagtatccaa
gcatcaccgaatactgttccatggg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCACACTACAACTATTCTCATATG 56 577 14 Antisense
ATTCTCATATGCTTTTTCATCTTTC 374 11 28 Antisense
TTCATCTTTCTTGTTCAAGTCTCCA 613 687 43 Antisense
AAGTCTCCACAATGGATGCTCACAC 522 157 59 Antisense
GGATGCTCACACTGACAAATACGTC 33 515 72 Antisense
AAATACGTCAGAACTCTGTGTGGAA 708 115 88 Antisense
CAGAAATATTGCCAACCTTTGCCCG 507 205 123 Antisense
TTTGCCCGCCAAAGCCAGAAATGAA 365 669 140 Antisense
GTATCTGTTCTACCGGAGAGTATCC 275 455 167 Antisense
GGAGAGTATCCAAGCATCACCGAAT 543 517 181 Antisense
GCATCACCGAATACTGTTCCATGGG 410 309 194 Antisense
Sequence Source Affymetrix Proprietary Database