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[188517_at] DNAJ domain

Description R74.4 /REP_DB=WormBase Gene ID /WP=CE01057 /TR=Q22028 /GB=CAA85274.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DNAJ domain
Cluster 6grp_highest_10_10 > 73      6grp_high1_10_10 > 37      4grp_highest_8_8 > 63      4grp_high_8_8 > 27      6grp_highest_nonorm > 73      6grp_total > 45      4grp_total > 29      6grp_highest_sub > Cu      6grp_cgc > 35      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 135
1 2 3 4 range
A 450.3
(-0.7)
P 		523
(3)
P 		406.2
(-3)
P 		488.6
(1.2)
P 		117
B 517.5
(2.6)
P 		491.8
(0.5)
P 		448.5
(-3)
P 		522.7
(3)
P 		74
C 541.6
(3)
P 		492.6
(-1.5)
P 		476.8
(-3)
P 		482.9
(-2.4)
P 		65
avr 503.1
(3) 		502.5
(2.9) 		443.8
(-3) 		498.1
(2.5) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 293
1 2 3 4 5 6 range
A 574
(1.5)
P 		524.7
(0.1)
P 		627.8
(3)
P 		531.1
(0.2)
P 		417.4
(-3)
P 		496.9
(-0.7)
P 		210
B 690.2
(3)
P 		502.8
(-0.8)
P 		642.4
(2)
P 		453.5
(-1.8)
P 		396.8
(-3)
P 		591.5
(1)
P 		293
C 582
(1.9)
P 		564.2
(1.2)
P 		521.7
(-0.3)
P 		503.2
(-1)
P 		447.8
(-3)
P 		612.9
(3)
P 		165
avr 615.4
(3) 		530.6
(0.4) 		597.3
(2.4) 		495.9
(-0.7) 		420.7
(-3) 		567.1
(1.5) 		195
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 108
1 2 3 4 range
A 163.9
(3)
P 		113.5
(-0.3)
P 		106.2
(-0.7)
P 		71.5
(-3)
P 		92
B 165
(3)
P 		121.5
(-1.3)
P 		103.9
(-3)
P 		111.4
(-2.3)
P 		61
C 179.3
(3)
P 		141.6
(0.7)
P 		166.2
(2.2)
P 		81.4
(-3)
P 		98
avr 169.4
(3) 		125.5
(-0.2) 		125.4
(-0.2) 		88.1
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 139
1 2 3 4 5 6 range
A 105.8
(-3)
P 		186.6
(3)
P 		136.7
(-0.7)
P 		134.7
(-0.9)
P 		125.9
(-1.5)
P 		121
(-1.9)
P 		81
B 240.1
(3)
P 		167.4
(-0.6)
P 		156
(-1.2)
P 		120.1
(-3)
P 		161.2
(-0.9)
P 		220.5
(2)
P 		120
C 215.7
(3)
P 		172.2
(0.7)
P 		132.8
(-1.3)
P 		101.2
(-3)
P 		144.9
(-0.7)
P 		205.2
(2.4)
P 		115
avr 187.2
(3) 		175.4
(2) 		141.8
(-1) 		118.7
(-3) 		144
(-0.8) 		182.2
(2.6) 		69
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188517_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6933
Sequence Type Exemplar sequence
Representative Public ID R74.4 NCBI
Target Description R74.4 /REP_DB=WormBase Gene ID /WP=CE01057 /TR=Q22028 /GB=CAA85274.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=DNAJ domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065438(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065438 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-16) (dnj-16) mRNA, complete cds. 11/11 A 
SNAP00000044748 ENSEMBL cdna:SNAP chromosome:CEL140:III:4191533:4193129:1 11/11 None
GENEFINDER00000044760 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4191533:4195088:1 11/11 None
R74.4 ENSEMBL cdna:known chromosome:CEL140:III:4191533:4193129:1 gene:R74.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4191532-4193129(+) 100.0 100.0
Public Domain and Genome References
Gene Title DNAJ domain
Gene Symbol dnj-16
Ensembl R74.4
Entrez Gene 175540 Entrez gene
SwissProt Q22028 EMBL-EBI
WormBase CE01057 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:264865_AT DNAJ heat shock protein, putative at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6457 protein folding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA85274 Hypothetical protein R74.4 [Caenorhabditis elegans] ref|NP_497839.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-16) [Caenorhabditis elegans] 0.0
blast CAE72865 Hypothetical protein CBG20164 [Caenorhabditis briggsae] 0.0
blast AAL60201 X-box binding protein processed isoform [Caenorhabditis elegans] 1.0E-116
blast XP_467717 putative Altered Response to Gravity [Oryza sativa (japonica cultivar-group)] ref|XP_506962.1| PREDICTED P0516G10.24 gene product [Oryza sativa (japonica cultivar-group)] dbj|BAD15765.1| putative Altered Response to Gravity [Oryza sativa (japonica cultivar-group)] dbj|BAD15722.1| putative Altered Response to Gravity [Oryza sativa (japonica cultivar-group)] 2.0E-46
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.00000003908296E-24
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 8.99999968283527E-25
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.8E-36
Sequence
Target Sequence 
>C. ELEGANS:188517_AT
gaggtgtccgaatgatccaagaaagtgggaaacgaggaaaagccggaactcaagccgaca
tgttctttgtaccgtacaatgtggccaatattcaagaattcaatgcaatgaaatatcatt
tggaggataaggaaactccattggcttttcattatttggatagttttgagatgcaaactg
cgacacttttggagacacggaagcattatattgcagtttatggtgataattggataagcg
atgtcaaatattcaataaccttccttcctgtggaaccttctgctgtcgagcaactgaatg
agattcaggatacggagaaatcgatttctattattaaaaaagaaatgctggatttccaaa
gagaattcacagaagctaagagaaaatatgatgaagctgtggcgaagttgaaggtacaag
acgacacaattctgaaagcgctggcacatcgtgaagagctctacaatgaagttctccaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGTGTCCGAATGATCCAAGAAAG 40 403 596 Antisense
GAAAAGCCGGAACTCAAGCCGACAT 81 353 632 Antisense
AAGCCGACATGTTCTTTGTACCGTA 609 149 647 Antisense
GTACCGTACAATGTGGCCAATATTC 470 457 664 Antisense
AACTCCATTGGCTTTTCATTATTTG 522 141 729 Antisense
ACTGCGACACTTTTGGAGACACGGA 235 99 772 Antisense
AATATTCAATAACCTTCCTTCCTGT 306 173 842 Antisense
AACCTTCTGCTGTCGAGCAACTGAA 708 145 869 Antisense
GACACAATTCTGAAAGCGCTGGCAC 125 367 1018 Antisense
AGCGCTGGCACATCGTGAAGAGCTC 493 81 1032 Antisense
GAGCTCTACAATGAAGTTCTCCAGA 570 387 1051 Antisense
Sequence Source Affymetrix Proprietary Database