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[188516_at] chitinase

Description R09D1.4 /REP_DB=WormBase Gene ID /WP=CE03545 /TR=Q21860 /GB=CAA93864.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=chitinase
Cluster 6grp_total > 65      4grp_total > 38      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 16
1 2 3 4 range
A 2.8
(-3)
A 		6.1
(0.7)
A 		8.2
(3)
A 		4.9
(-0.7)
A 		5
B 17.4
(3)
A 		3.9
(-2.2)
A 		1.7
(-3)
A 		6.7
(-1.1)
A 		16
C 4.2
(0.9)
A 		5.9
(3)
A 		1.1
(-3)
A 		5.8
(2.9)
A 		5
avr 8.1
(3) 		5.3
(-0.8) 		3.7
(-3) 		5.8
(-0.1) 		4

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 9.3
(-1.7)
A 		4
(-2.7)
A 		24.1
(1.2)
A 		2.3
(-3)
A 		5.4
(-2.4)
A 		33.6
(3)
A 		31
B 1.1
(-3)
A 		2.1
(-1.9)
A 		1.7
(-2.3)
A 		6.6
(3)
A 		4.8
(1)
A 		2.8
(-1.1)
A 		6
C 3
(-2.2)
A 		11.6
(2.2)
A 		3.6
(-1.9)
A 		13.2
(3)
A 		3.5
(-2)
A 		1.5
(-3)
A 		12
avr 4.5
(-3) 		5.9
(-2) 		9.8
(0.9) 		7.4
(-0.9) 		4.6
(-2.9) 		12.6
(3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 1
(-1.7)
A 		1.3
(-0.4)
A 		2.1
(3)
A 		0.7
(-3)
A 		1
B 5.5
(3)
A 		1
(-2.3)
A 		0.4
(-3)
A 		1.4
(-1.8)
A 		5
C 1.4
(1.6)
A 		1.7
(3)
A 		0.4
(-3)
A 		1
(-0.2)
A 		1
avr 2.6
(3) 		1.3
(-1.9) 		1
(-3) 		1
(-3) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 8
1 2 3 4 5 6 range
A 1.7
(-2.1)
A 		1.4
(-2.4)
A 		5.3
(0.7)
A 		0.6
(-3)
A 		1.6
(-2.2)
A 		8.2
(3)
A 		8
B 0.4
(-3)
A 		0.7
(-1.9)
A 		0.4
(-3)
A 		1.7
(1.9)
A 		2
(3)
A 		1
(-0.8)
A 		2
C 1.1
(-1.8)
A 		3.6
(3)
A 		0.9
(-2.2)
A 		2.6
(1.1)
A 		1.1
(-1.8)
A 		0.5
(-3)
A 		3
avr 1.1
(-3) 		1.9
(-0.7) 		2.2
(0.1) 		1.6
(-1.6) 		1.6
(-1.6) 		3.2
(3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188516_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2846
Sequence Type Exemplar sequence
Representative Public ID R09D1.4 NCBI
Target Description R09D1.4 /REP_DB=WormBase Gene ID /WP=CE03545 /TR=Q21860 /GB=CAA93864.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=chitinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063622(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063622 NCBI Caenorhabditis elegans R09D1.4 (chitinase) mRNA, complete cds. 11/11 None
SNAP00000010206 ENSEMBL cdna:SNAP chromosome:CEL140:II:9445999:9454206:-1 10/11 None
GENEFINDER00000010222 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9445999:9447614:-1 9/11 None
R09D1.4 ENSEMBL cdna:known chromosome:CEL140:II:9445999:9447614:-1 gene:R09D1.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9445995-9447332(-) 100.0 100.0
Public Domain and Genome References
Gene Title chitinase
Ensembl R09D1.4
Entrez Gene 187734 Entrez gene
SwissProt Q21860 EMBL-EBI
WormBase CE28638 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93864 Hypothetical protein R09D1.4 [Caenorhabditis elegans] ref|NP_496023.1| R09D1.4 [Caenorhabditis elegans] 0.0
blast CAA93866 Hypothetical protein R09D1.6 [Caenorhabditis elegans] ref|NP_496026.1| R09D1.6 [Caenorhabditis elegans] 0.0
blast CAA93868 Hypothetical protein R09D1.8 [Caenorhabditis elegans] ref|NP_496029.1| R09D1.8 [Caenorhabditis elegans] 0.0
blast CAA93871 Hypothetical protein R09D1.12 [Caenorhabditis elegans] ref|NP_496025.1| R09D1.12 [Caenorhabditis elegans] 0.0
blast CAA93865 Hypothetical protein R09D1.5 [Caenorhabditis elegans] ref|NP_496024.2| R09D1.5 [Caenorhabditis elegans] 1.0E-61
blast CAB05568 Hypothetical protein M01B2.6 [Caenorhabditis elegans] ref|NP_506770.2| M01B2.6 [Caenorhabditis elegans] 1.0E-59
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.8E-31
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 1.2E-99
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 1.5E-81
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 2.6E-42
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 2.4E-23
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 4.1E-85
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496023.1 1 29-51
R09D1.4 1 29-51
SNAP00000010206 1 35-57
GENEFINDER00000010222 1 29-51
Sequence
Target Sequence 
>C. ELEGANS:188516_AT
agatgtctacgtatcatttcttcgtaatttacataaagctttacaaagagaattagaggg
attaagagatccactgacattgtctgtagttgttccacactacataaatcaccgagaggc
tggatacagtataccggatattttgaagtacgttgattttgtgaacgttttcacaatgga
ttattatggaccgagtgatggctctactggacctttgtctcctttgtactccggacacct
ggacaaaaaactaaatgtggcagatgctgatgaaaacagtgaggaaagtgcctatatgtc
ccgaaaaacagtcgctaaagagaagatccgactaactcttccatcgtgggataccttaac
tgagacttcttctatgtacaataactcaacgaagacatttctggcatttgaaaccgagaa
aagtatacatgcaaaaatagaatatgtgatgaacaagaatttgggtggagtttggatttg
gtcagtggatatggatgatgaacgttatcacagtttgctgagtactattccatcaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGATGTCTACGTATCATTTCTTCGT 30 67 567 Antisense
GACATTGTCTGTAGTTGTTCCACAC 441 371 642 Antisense
GTTCCACACTACATAAATCACCGAG 83 441 658 Antisense
TGGACCGAGTGATGGCTCTACTGGA 71 547 753 Antisense
TTGTACTCCGGACACCTGGACAAAA 652 707 790 Antisense
GAAAGTGCCTATATGTCCCGAAAAA 606 357 850 Antisense
GAAGATCCGACTAACTCTTCCATCG 407 337 888 Antisense
AACTCTTCCATCGTGGGATACCTTA 33 141 900 Antisense
GGATACCTTAACTGAGACTTCTTCT 222 509 915 Antisense
GATGAACGTTATCACAGTTTGCTGA 84 413 1063 Antisense
AGTTTGCTGAGTACTATTCCATCAA 430 63 1078 Antisense
Sequence Source Affymetrix Proprietary Database