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[188494_s_at]

Description F08B6.4 /REP_DB=WormBase Gene ID /WP=CE20658 /CHR=1 /FEA=Sanger Annotation /DEF=calponin (ST.LOUIS) TR:Q9TYS6 protein_id:AAC78234.1
Cluster 6grp_highest_10_10 > 18      6grp_high1_10_10 > 80      4grp_highest_8_8 > 16      4grp_high_8_8 > 52      6grp_highest_nonorm > 27      6grp_total > 92      4grp_total > 59      6grp_highest_sub > 04_05      6grp_cgc > 97      6grp_high1_sub > 02_03_04_05      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6673
1 2 3 4 range
A 6315
(0.8)
P 		8633.1
(3)
P 		8336.2
(2.7)
P 		2420.7
(-3)
P 		6212
B 6746.2
(1.2)
P 		8652.1
(3)
P 		8675.6
(3)
P 		2324.4
(-3)
P 		6351
C 6942.7
(1.5)
P 		8579.6
(3)
P 		7984.4
(2.5)
P 		2002.8
(-3)
P 		6577
avr 6668
(1.2) 		8621.6
(3) 		8332.1
(2.7) 		2249.3
(-3) 		6372

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2932
1 2 3 4 5 6 range
A 7742.8
(1.7)
P 		7578.3
(1.3)
P 		7384.6
(0.8)
P 		7667.7
(1.5)
P 		8298.4
(3)
P 		5799.9
(-3)
P 		2499
B 7327.9
(0.6)
P 		7109.4
(0.1)
P 		6831.6
(-0.5)
P 		8066.6
(2.3)
P 		8384.3
(3)
P 		5733.5
(-3)
P 		2651
C 8144.6
(2.1)
P 		6691.4
(-0.8)
P 		7544.2
(0.9)
P 		8008.6
(1.9)
P 		8562.3
(3)
P 		5630.7
(-3)
P 		2932
avr 7738.4
(1.5) 		7126.4
(0.1) 		7253.5
(0.4) 		7914.3
(1.9) 		8415
(3) 		5721.4
(-3) 		2694
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2445
1 2 3 4 range
A 2299.3
(3)
P 		1874
(1.7)
P 		2179.2
(2.6)
P 		354.3
(-3)
P 		1945
B 2151.3
(3)
P 		2136.9
(2.9)
P 		2010.3
(2.5)
P 		495.3
(-3)
P 		1656
C 2298.2
(1.8)
P 		2466.7
(2.2)
P 		2782.8
(3)
P 		337.7
(-3)
P 		2445
avr 2249.6
(2.8) 		2159.2
(2.5) 		2324.1
(3) 		395.8
(-3) 		1928

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1994
1 2 3 4 5 6 range
A 1427.3
(-2.9)
P 		2695.2
(3)
P 		1608.2
(-2.1)
P 		1944.7
(-0.5)
P 		2504.1
(2.1)
P 		1412.1
(-3)
P 		1283
B 2549.7
(0.1)
P 		2366.4
(-0.6)
P 		1658.9
(-3)
P 		2136.6
(-1.4)
P 		3406.5
(3)
P 		2138
(-1.4)
P 		1748
C 3018.8
(3)
P 		2041.7
(-1.2)
P 		1920.1
(-1.7)
P 		1610.5
(-3)
P 		2770.6
(1.9)
P 		1884.9
(-1.8)
P 		1408
avr 2331.9
(0.1) 		2367.8
(0.3) 		1729.1
(-3) 		1897.3
(-2.1) 		2893.7
(3) 		1811.7
(-2.6) 		1165
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188494_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.459
Sequence Type Exemplar sequence
Representative Public ID F08B6.4 NCBI
Target Description F08B6.4 /REP_DB=WormBase Gene ID /WP=CE20658 /CHR=1 /FEA=Sanger Annotation /DEF=calponin (ST.LOUIS) TR:Q9TYS6 protein_id:AAC78234.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq,GenBank identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001025923(11), NM_001025922(11), NM_001025921(11), U04711(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001025923 NCBI Caenorhabditis elegans UNCoordinated family member (unc-87) (unc-87) mRNA, complete cds. 11/11 None
NM_001025922 NCBI Caenorhabditis elegans UNCoordinated family member (unc-87) (unc-87) mRNA, complete cds. 11/11 None
NM_001025921 NCBI Caenorhabditis elegans UNCoordinated family member (unc-87) (unc-87) mRNA, complete cds. 11/11 None
U04711 NCBI Caenorhabditis elegans N2 (unc-87) mRNA, complete cds. 11/11 A 
SNAP00000014331 ENSEMBL cdna:SNAP chromosome:CEL140:I:6769901:6773744:-1 11/11 None
GENEFINDER00000014337 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6770773:6773744:-1 11/11 None
F08B6.4c.3 ENSEMBL cdna:known chromosome:CEL140:I:6769362:6775931:-1 gene:F08B6.4 11/11 A 
F08B6.4c.2 ENSEMBL cdna:known chromosome:CEL140:I:6769362:6773745:-1 gene:F08B6.4 11/11 A 
F08B6.4c.1 ENSEMBL cdna:known chromosome:CEL140:I:6769362:6773846:-1 gene:F08B6.4 11/11 A 
F08B6.4b.1 ENSEMBL cdna:known chromosome:CEL140:I:6769701:6775931:-1 gene:F08B6.4 11/11 None
F08B6.4a ENSEMBL cdna:known chromosome:CEL140:I:6769901:6773744:-1 gene:F08B6.4 11/11 A 
F08B6.4b.2 ENSEMBL cdna:known chromosome:CEL140:I:6769901:6775933:-1 gene:F08B6.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6769829-6773673(-) 100.0 100.0
Public Domain and Genome References
Ensembl F08B6.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAT68897 Uncoordinated protein 87, isoform c [Caenorhabditis elegans] ref|NP_001021094.1| UNCoordinated family member (unc-87) [Caenorhabditis elegans] 0.0
blast AAC78234 Uncoordinated protein 87, isoform a [Caenorhabditis elegans] ref|NP_001021092.1| UNCoordinated family member (unc-87) [Caenorhabditis elegans] pir||T33851 thin filament-associated protein UNC-87, long form - Caenorhabditis elegans gb|AAA81902.1| one of two alternatively spliced products 0.0
blast P37806 Uncoordinated protein 87 (Protein unc-87) 0.0
blast AAK68303 Uncoordinated protein 87, isoform b [Caenorhabditis elegans] ref|NP_001021093.1| UNCoordinated family member (unc-87) [Caenorhabditis elegans] gb|AAA81901.1| uses the first of two potential start codons; second of two alternatively spliced products gb|AAA81899.1| uses first of two potential start sites 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000557 EMBL-EBI Calponin repeat 1.4E-4
Pfam IPR000557 EMBL-EBI Calponin repeat 2.3E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 4.2E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 1.0E-10
Pfam IPR000557 EMBL-EBI Calponin repeat 5.5E-9
Pfam IPR000557 EMBL-EBI Calponin repeat 3.8E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 7.0E-5
Pfam IPR000557 EMBL-EBI Calponin repeat 1.4E-4
Pfam IPR000557 EMBL-EBI Calponin repeat 2.3E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 4.2E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 1.0E-10
Pfam IPR000557 EMBL-EBI Calponin repeat 5.5E-9
Pfam IPR000557 EMBL-EBI Calponin repeat 3.8E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 7.0E-5
Pfam IPR000557 EMBL-EBI Calponin repeat 1.4E-4
Pfam IPR000557 EMBL-EBI Calponin repeat 2.3E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 4.2E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 1.0E-10
Pfam IPR000557 EMBL-EBI Calponin repeat 5.5E-9
Pfam IPR000557 EMBL-EBI Calponin repeat 3.8E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 7.0E-5
Pfam IPR000557 EMBL-EBI Calponin repeat 1.4E-4
Pfam IPR000557 EMBL-EBI Calponin repeat 2.3E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 4.2E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 1.0E-10
Pfam IPR000557 EMBL-EBI Calponin repeat 5.5E-9
Pfam IPR000557 EMBL-EBI Calponin repeat 3.8E-8
Pfam IPR000557 EMBL-EBI Calponin repeat 7.0E-5
Sequence
Target Sequence 
>C. ELEGANS:188494_S_AT
ggaactggacgtcgtgagacaactaagatggttgactcaaagcatccagaatacgatcac
gagaaaccagatcaatctgaaattccacttcagtctggaacaaacaaattcgcttcacaa
aagggaatgaccggtttcggaactgctcgtcgtgagaccacaaagatggttgattctaac
catccagattactctcatgaatgttccattgatcaaaccacaattccatctcagatggga
tctaatcaatatgcttcacagaagggtatgactggattcggacagccacgttgggaagtt
ctcgacccatcaatctcatggcaaaaccgtaaatcccagggaatggtccgtcttcaatct
ggtaccaaccgttttgcttctcaagctggtatgattggcttcggaacatgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAACTGGACGTCGTGAGACAACTA 95 529 1081 Antisense
GGTTGACTCAAAGCATCCAGAATAC 457 507 1110 Antisense
GTTTCGGAACTGCTCGTCGTGAGAC 269 449 1214 Antisense
GATTCTAACCATCCAGATTACTCTC 108 431 1252 Antisense
TCAAACCACAATTCCATCTCAGATG 524 629 1293 Antisense
ATGACTGGATTCGGACAGCCACGTT 38 39 1348 Antisense
GCCACGTTGGGAAGTTCTCGACCCA 624 279 1365 Antisense
TCTCGACCCATCAATCTCATGGCAA 362 611 1380 Antisense
AATGGTCCGTCTTCAATCTGGTACC 6 161 1422 Antisense
ATCTGGTACCAACCGTTTTGCTTCT 201 23 1437 Antisense
GGTATGATTGGCTTCGGAACATGCA 166 503 1468 Antisense
Sequence Source Affymetrix Proprietary Database