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[188491_at] methylase

Description C38D4.9 /REP_DB=WormBase Gene ID /WP=CE00922 /TR=Q18511 /GB=CAA86320.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=methylase
Cluster 6grp_high1_10_10 > 74      4grp_high_8_8 > 04      6grp_total > 64      4grp_total > 12      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 367
1 2 3 4 range
A 419.4
(-0.8)
P 		286.8
(-3)
P 		294.5
(-2.9)
A 		653.9
(3)
P 		367
B 428.4
(0.4)
P 		411.9
(-0)
A 		301.1
(-3)
A 		523.2
(3)
P 		222
C 439.3
(-0.9)
P 		383.3
(-1.9)
P 		322.9
(-3)
P 		652.9
(3)
P 		330
avr 429
(-0.6) 		360.7
(-1.9) 		306.2
(-3) 		610
(3) 		304

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 254
1 2 3 4 5 6 range
A 378.1
(0.2)
A 		308.1
(-3)
P 		438.8
(3)
P 		361
(-0.6)
P 		370.6
(-0.1)
P 		355
(-0.8)
P 		131
B 314.3
(-2.3)
P 		402.3
(1.6)
P 		435
(3)
P 		346.7
(-0.9)
P 		299
(-3)
P 		405.1
(1.7)
P 		136
C 264.8
(-3)
P 		287.6
(-2.5)
P 		328.3
(-1.5)
P 		519
(3)
P 		277
(-2.7)
P 		464.2
(1.7)
P 		254
avr 319.1
(-2.8) 		332.7
(-1.9) 		400.7
(2.5) 		408.9
(3) 		315.5
(-3) 		408.1
(2.9) 		93
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 90
1 2 3 4 range
A 152.7
(3)
P 		62.3
(-3)
P 		77
(-2)
A 		95.7
(-0.8)
P 		90
B 136.6
(3)
P 		101.7
(-0.1)
A 		69.8
(-3)
A 		111.5
(0.7)
P 		67
C 145.4
(3)
P 		110.2
(-3)
P 		112.5
(-2.6)
P 		110.1
(-3)
P 		35
avr 144.9
(3) 		91.4
(-2.5) 		86.4
(-3) 		105.8
(-1) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 86
1 2 3 4 5 6 range
A 69.7
(-3)
A 		109.6
(2.7)
P 		95.6
(0.7)
P 		91.6
(0.1)
P 		111.8
(3)
P 		86.4
(-0.6)
P 		42
B 109.4
(-1.2)
P 		133.9
(1.3)
P 		105.6
(-1.6)
P 		91.8
(-3)
P 		121.5
(0)
P 		151
(3)
P 		59
C 98.2
(-1.8)
P 		87.8
(-2.6)
P 		83.6
(-3)
P 		104.4
(-1.3)
P 		89.6
(-2.5)
P 		155.4
(3)
P 		72
avr 92.4
(-3) 		110.4
(-0.2) 		94.9
(-2.6) 		95.9
(-2.5) 		107.6
(-0.6) 		130.9
(3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188491_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6524
Sequence Type Exemplar sequence
Representative Public ID C38D4.9 NCBI
Target Description C38D4.9 /REP_DB=WormBase Gene ID /WP=CE00922 /TR=Q18511 /GB=CAA86320.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=methylase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065589(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065589 NCBI Caenorhabditis elegans C38D4.9 (methylase) mRNA, complete cds. 11/11 None
SNAP00000018667 ENSEMBL cdna:SNAP chromosome:CEL140:III:4802969:4804648:1 11/11 None
GENEFINDER00000018678 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:4802969:4804648:1 11/11 None
C38D4.9.1 ENSEMBL cdna:known chromosome:CEL140:III:4802966:4804709:1 gene:C38D4.9 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:4802968-4804648(+) 100.0 100.0
Public Domain and Genome References
Gene Title methylase
Ensembl C38D4.9
Entrez Gene 175637 Entrez gene
SwissProt Q18511 EMBL-EBI
WormBase CE00922 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:253762_AT expressed protein at
CANINE:1597667_AT similar to CG9666-PA cfa
CANINE:1591867_S_AT similar to CG9666-PA cfa
CANINE:1591866_AT similar to CG9666-PA cfa
CANINE:1592414_AT similar to CG9666-PA cfa
CANINE_2:CFA.10084.1.S1_S_AT similar to CG9666-PA cfa
CANINE_2:CFA.3271.1.S1_AT similar to CG9666-PA cfa
CANINE_2:CFAAFFX.19300.1.S1_S_AT similar to CG9666-PA cfa
DROSOPHILA_2:1633418_AT dm
DROSGENOME1:154328_AT dm
CHICKEN:GGA.10171.1.S1_AT similar to 2810410A08Rik protein gga
CHICKEN:GGA.19126.1.S1_AT similar to 2810410A08Rik protein gga
MOUSE430A_2:1460519_A_AT methyltransferase like 5 mm
MOE430A:1460519_A_AT methyltransferase like 5 mm
MOUSE430_2:1460519_A_AT methyltransferase like 5 mm
MG-U74AV2:92191_AT methyltransferase like 5 mm
MG-U74BV2:165335_I_AT methyltransferase like 5 mm
MU19KSUBC:TC41875_AT Methyltransferase like 5, mRNA (cDNA clone MGC:41295 IMAGE:1531973) mm
RG-U34C:RC_AI235885_AT similar to 2810410A08Rik protein rn
RAE230A:1367541_AT similar to 2810410A08Rik protein rn
RAE230B:1367541_AT similar to 2810410A08Rik protein rn
RAT230_2:1367541_AT similar to 2810410A08Rik protein rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6306 DNA methylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
8170 N-methyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86320 Hypothetical protein C38D4.9 [Caenorhabditis elegans] ref|NP_497990.1| C38D4.9 [Caenorhabditis elegans] 1.0E-121
blast CAE71127 Hypothetical protein CBG17981 [Caenorhabditis briggsae] 1.0E-95
blast XP_788985 PREDICTED: similar to CG9666-PA [Strongylocentrotus purpuratus] 7.0E-42
Sequence
Target Sequence 
>C. ELEGANS:188491_AT
agagcttgccgtctcgatgatggaaatgatcgatgaaacaattggttttgaaggaaagaa
attaattgatatcggatgtggatgtggaatgttaatgacgacagctgccacaatgtatga
gttggagactgttttaggagttgatatagatgatgaagcacttaaaatttgttctagaaa
tttggaaacagctgaagtacaggatagatgcgagcttctacaagccgacattctcgaccc
ggaatccgatcttccacgtggcacattcgacgtggctgtcattaatcctccatttggaac
aaaaaataatgcaggaatcgatatgcaatttgtccaaatcggtcttcaaatggttcggcc
tggtggaagtgtattttcacttcataaatcatcaactagagattatattctaaaaaatgc
aaagaaatgggacggagttggggcagaatgttgtgcagaaatgcggtggcaactaccagc
gacttacaaattccataaacaaaaggccgtggatattgccgtcgat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCTTGCCGTCTCGATGATGGAA 75 69 102 Antisense
ATGACGACAGCTGCCACAATGTATG 207 45 196 Antisense
GGATAGATGCGAGCTTCTACAAGCC 681 509 303 Antisense
TTCTACAAGCCGACATTCTCGACCC 409 691 317 Antisense
ATCTTCCACGTGGCACATTCGACGT 389 29 350 Antisense
TGGCACATTCGACGTGGCTGTCATT 287 545 360 Antisense
GGCTGTCATTAATCCTCCATTTGGA 572 539 375 Antisense
GTCTTCAAATGGTTCGGCCTGGTGG 225 467 443 Antisense
AAATGCGGTGGCAACTACCAGCGAC 619 117 560 Antisense
ACTACCAGCGACTTACAAATTCCAT 454 101 573 Antisense
AAAGGCCGTGGATATTGCCGTCGAT 356 121 603 Antisense
Sequence Source Affymetrix Proprietary Database