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[188489_s_at] helicase

Description ZC317.1 /REP_DB=WormBase Gene ID /WP=CE15167 /TR=Q23080 /GB=AAB04965.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=helicase
Cluster 6grp_highest_10_10 > 69      6grp_high1_10_10 > 96      4grp_highest_8_8 > 22      4grp_high_8_8 > 15      6grp_highest_nonorm > 91      6grp_total > 96      4grp_total > 01      6grp_highest_sub > In      6grp_cgc > 68      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 204
1 2 3 4 range
A 110.4
(-1.7)
P 		79.7
(-3)
P 		116.4
(-1.5)
P 		227
(3)
P 		147
B 114.8
(-2.4)
P 		108.5
(-3)
P 		152.7
(1.1)
P 		172.6
(3)
P 		64
C 99.3
(-3)
P 		133.9
(-1.9)
P 		115.3
(-2.5)
P 		283.2
(3)
P 		184
avr 108.2
(-3) 		107.4
(-3) 		128.1
(-2) 		227.6
(3) 		120

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 276
1 2 3 4 5 6 range
A 90.6
(-3)
P 		123.1
(-2)
P 		155
(-1)
P 		167.7
(-0.6)
P 		274.3
(2.8)
P 		280.9
(3)
P 		190
B 91.5
(-3)
P 		116.2
(-2.5)
P 		221
(-0.1)
P 		232.9
(0.1)
P 		270.8
(1)
P 		362.4
(3)
P 		271
C 117.8
(-3)
P 		133.2
(-2.6)
P 		167.8
(-1.8)
P 		200.8
(-1)
P 		270.2
(0.7)
P 		366.5
(3)
P 		249
avr 100
(-3) 		124.2
(-2.4) 		181.3
(-0.9) 		200.5
(-0.5) 		271.8
(1.4) 		336.6
(3) 		237
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 40.2
(3)
P 		17.3
(-3)
P 		30.4
(0.4)
P 		33.2
(1.2)
P 		23
B 36.6
(2.9)
P 		26.8
(-3)
P 		35.4
(2.2)
P 		36.8
(3)
P 		10
C 32.9
(-3)
P 		38.5
(-0.7)
P 		40.2
(-0.1)
P 		47.8
(3)
P 		15
avr 36.6
(1.6) 		27.5
(-3) 		35.3
(1) 		39.3
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 16.7
(-3)
P 		43.8
(-0.5)
P 		33.8
(-1.4)
P 		42.5
(-0.7)
P 		82.8
(3)
P 		68.4
(1.7)
P 		66
B 31.8
(-3)
P 		38.7
(-2.6)
P 		53.7
(-1.7)
P 		61.7
(-1.3)
P 		110
(1.5)
P 		135.1
(3)
P 		103
C 43.7
(-2.8)
P 		40.6
(-3)
P 		42.7
(-2.8)
P 		40.4
(-3)
P 		87.4
(0.4)
P 		122.7
(3)
P 		82
avr 30.7
(-3) 		41
(-2.2) 		43.4
(-2) 		48.2
(-1.7) 		93.4
(1.8) 		108.7
(3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188489_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12250
Sequence Type Exemplar sequence
Representative Public ID ZC317.1 NCBI
Target Description ZC317.1 /REP_DB=WormBase Gene ID /WP=CE15167 /TR=Q23080 /GB=AAB04965.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=helicase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072046(11), NM_072089(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072046 NCBI Caenorhabditis elegans ZC317.1 (helicase) mRNA, complete cds. 11/11 None
NM_072089 NCBI Caenorhabditis elegans C14C11.6 (helicase) mRNA, complete cds. 11/11 A 
SNAP00000024122 ENSEMBL cdna:SNAP chromosome:CEL140:V:5661469:5663299:-1 11/11 None
GENEFINDER00000024135 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5661469:5663299:-1 11/11 None
SNAP00000026644 ENSEMBL cdna:SNAP chromosome:CEL140:V:5471591:5472956:1 11/11 None
GENEFINDER00000026649 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:5471654:5472886:1 11/11 None
ZC317.1 ENSEMBL cdna:known chromosome:CEL140:V:5471591:5472956:1 gene:ZC317.1 11/11 None
C14C11.6 ENSEMBL cdna:known chromosome:CEL140:V:5661371:5663299:-1 gene:C14C11.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:5471590-5472956(+) 100.0 100.0
chrV:5661654-5663309(-) 90.22 90.22
Public Domain and Genome References
Gene Title helicase
helicase
Gene Symbol mut-14
Ensembl ZC317.1
C14C11.6
Entrez Gene 178952 Entrez gene
191138 Entrez gene
SwissProt Q17978 EMBL-EBI
Q23080 EMBL-EBI
WormBase CE06825 Wormbase
CE15167 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3676 nucleic acid binding inferred from electronic annotation QuickGO AmiGO
4386 helicase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
8026 ATP-dependent helicase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB04965 Hypothetical protein ZC317.1 [Caenorhabditis elegans] ref|NP_504447.1| ZC317.1 [Caenorhabditis elegans] 0.0
blast AAA96106 Mutator protein 14 [Caenorhabditis elegans] ref|NP_504490.1| C14C11.6 [Caenorhabditis elegans] 0.0
blast AAK95885 Hypothetical protein Y38A10A.6 [Caenorhabditis elegans] ref|NP_504574.1| Y38A10A.6 [Caenorhabditis elegans] 1.0E-180
Protein Domains
Database ID Description E-Value
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.9E-15
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 6.3E-40
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 5.5E-20
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 1.1E-45
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.9E-15
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 6.3E-41
Pfam IPR001650 EMBL-EBI Helicase, C-terminal 1.9E-15
Pfam IPR001410 EMBL-EBI DEAD/DEAH box helicase 2.5E-38
Sequence
Target Sequence 
>C. ELEGANS:188489_S_AT
gaaagcgacgattgaatgtggcgacgtatgccttgataatttgaaaacgattattctgga
cgaagctgataaaatgatcgacaattgtgctttcgttcccgacatcaattggatcctgga
tcaactgaaggaagaaaacctggaaaatctacagtcgtgcttcttctcggcaacctataa
taaaaatgcgaatggcacaattgcattgactgtacttcaaatgagaatgttgggagaaga
cgatccgtgtaaagatggaccttataatgagacgattgccattttttgtgagactgtcca
aagagttactcaggtaaccatgactctccgtcaattgggatacaatttcaagcccgtgtg
cagcatgatcacgaaaaatcagcagctcgtcactgttaatgatttggaattccgaaaaat
tcacggagtcgtctgcaccaacatcctggcaagaggaattgacgtttcatctgtgaagca
tacaatcatcatggaaatgtcggcagactttgacacctacaagcacaggattggta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAGCGACGATTGAATGTGGCGAC 386 359 414 Antisense
GAATGTGGCGACGTATGCCTTGATA 227 331 427 Antisense
CCCGACATCAATTGGATCCTGGATC 416 261 511 Antisense
GGAAAATCTACAGTCGTGCTTCTTC 70 529 555 Antisense
GCTTCTTCTCGGCAACCTATAATAA 541 305 572 Antisense
GGCACAATTGCATTGACTGTACTTC 301 535 607 Antisense
GACGATTGCCATTTTTTGTGAGACT 666 377 684 Antisense
GAGACTGTCCAAAGAGTTACTCAGG 340 393 703 Antisense
AAATCAGCAGCTCGTCACTGTTAAT 167 117 789 Antisense
ATGTCGGCAGACTTTGACACCTACA 391 49 910 Antisense
GACACCTACAAGCACAGGATTGGTA 43 369 925 Antisense
Sequence Source Affymetrix Proprietary Database