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[188478_at]

Description M04D8.3 /REP_DB=WormBase Gene ID /WP=CE00578 /GEN=ins-23 /TR=SW:Q21506 /GB=CAA83609.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_high1_10_10 > 80      4grp_high_8_8 > 46      6grp_total > 82      4grp_total > 50      6grp_cgc > 97      6grp_high1_sub > 02_03_04_05      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 63.8
(-0.8)
P 		124.3
(3)
P 		103.4
(1.7)
P 		28.5
(-3)
A 		96
B 69.2
(-2.7)
P 		124.3
(3)
M 		101.6
(0.7)
P 		66.3
(-3)
P 		58
C 82.9
(-3)
P 		88.7
(-0.1)
P 		95.1
(3)
P 		84.8
(-2.1)
P 		12
avr 72
(-1.6) 		112.4
(3) 		100
(1.6) 		59.9
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 84
1 2 3 4 5 6 range
A 82.2
(-2.7)
P 		117.7
(3)
P 		95.2
(-0.6)
P 		98.3
(-0.1)
A 		110.4
(1.8)
P 		80.5
(-3)
A 		37
B 45.4
(-3)
A 		72.5
(-0.7)
P 		74.8
(-0.5)
P 		78.1
(-0.2)
P 		116.4
(3)
P 		45.7
(-3)
M 		71
C 69
(-1.2)
A 		63.9
(-1.5)
A 		65.3
(-1.4)
A 		127.8
(3)
A 		99.9
(1)
P 		43.4
(-3)
P 		84
avr 65.5
(-2) 		84.7
(0.2) 		78.4
(-0.5) 		101.4
(2.1) 		108.9
(3) 		56.5
(-3) 		52
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 29
1 2 3 4 range
A 23.2
(2)
P 		27
(3)
P 		27
(3)
P 		4.2
(-3)
A 		23
B 22.1
(-0.1)
P 		30.7
(3)
M 		23.5
(0.4)
P 		14.1
(-3)
P 		17
C 27.4
(1.2)
P 		25.5
(0.6)
P 		33.2
(3)
P 		14.3
(-3)
P 		19
avr 24.2
(1.7) 		27.7
(2.9) 		27.9
(3) 		10.9
(-3) 		17

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 15.1
(-3)
P 		41.8
(3)
P 		20.7
(-1.7)
P 		24.9
(-0.8)
A 		33.3
(1.1)
P 		19.6
(-2)
A 		27
B 15.8
(-3)
A 		24.1
(-1.4)
P 		18.2
(-2.5)
P 		20.7
(-2.1)
P 		47.3
(3)
P 		17
(-2.8)
M 		32
C 25.6
(0.7)
A 		19.5
(-1.3)
A 		16.6
(-2.3)
A 		25.7
(0.8)
A 		32.3
(3)
P 		14.5
(-3)
P 		18
avr 18.8
(-2.5) 		28.5
(0.3) 		18.5
(-2.6) 		23.8
(-1) 		37.6
(3) 		17
(-3) 		21
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188478_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7969
Sequence Type Exemplar sequence
Representative Public ID M04D8.3 NCBI
Target Description M04D8.3 /REP_DB=WormBase Gene ID /WP=CE00578 /GEN=ins-23 /TR=SW:Q21506 /GB=CAA83609.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066823(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066823 NCBI Caenorhabditis elegans INSulin related family member (ins-23) (ins-23) mRNA, complete cds. 11/11 None
SNAP00000022942 ENSEMBL cdna:SNAP chromosome:CEL140:III:10028156:10028444:1 11/11 None
M04D8.3 ENSEMBL cdna:known chromosome:CEL140:III:10028156:10028444:1 gene:M04D8.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10028151-10028440(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-23
Ensembl M04D8.3
Entrez Gene 191691 Entrez gene
SwissProt Q21506 EMBL-EBI
WormBase CE00578 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83609 Hypothetical protein M04D8.3 [Caenorhabditis elegans] ref|NP_499224.1| INSulin related family member (ins-23) [Caenorhabditis elegans] sp|Q21506|ILA3_CAEEL Probable insulin-like peptide alpha-type 3 precursor 2.0E-38
blast CAA83611 Hypothetical protein M04D8.2 [Caenorhabditis elegans] ref|NP_499223.1| INSulin related family member (ins-22) [Caenorhabditis elegans] sp|Q21508|ILA2_CAEEL Probable insulin-like peptide alpha-type 2 precursor 9.0E-15
blast CAE65066 Hypothetical protein CBG09916 [Caenorhabditis briggsae] 9.0E-13
Sequence
Target Sequence 
>C. ELEGANS:188478_AT
atgttcgttcttcttattattctctctatcattctggctcaagtcactgatgctcattca
gagcttcacgttcgtagggtgtgcggaactgctatcataaagaacataatgcgattgtgc
ccaggggtaccggcttgcgaaaatggagaagttccaagtccaaccgagtactgttcaatg
gggtactcagacagccaggta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTCGTTCTTCTTATTATTCTCT 587 47 13 Antisense
CTCTCTATCATTCTGGCTCAAGTCA 343 231 34 Antisense
GGCTCAAGTCACTGATGCTCATTCA 326 539 48 Antisense
CTCATTCAGAGCTTCACGTTCGTAG 453 229 65 Antisense
GAGCTTCACGTTCGTAGGGTGTGCG 120 389 73 Antisense
AGGGTGTGCGGAACTGCTATCATAA 284 59 88 Antisense
TAATGCGATTGTGCCCAGGGGTACC 363 625 119 Antisense
CAGGGGTACCGGCTTGCGAAAATGG 54 3 134 Antisense
GAAGTTCCAAGTCCAACCGAGTACT 353 337 160 Antisense
AGTCCAACCGAGTACTGTTCAATGG 154 61 169 Antisense
AATGGGGTACTCAGACAGCCAGGTA 624 159 189 Antisense
Sequence Source Affymetrix Proprietary Database