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[188477_at] cuticulin

Description ZK265.8 /REP_DB=WormBase Gene ID /WP=CE15303 /TR=Q93967 /GB=CAB00057.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=cuticulin
Cluster 6grp_total > 82      4grp_total > 57      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 123
1 2 3 4 range
A 47.7
(-1.2)
A 		91.7
(1)
A 		131.1
(3)
A 		12.3
(-3)
A 		119
B 48.4
(-2.4)
A 		119.3
(2)
A 		134.9
(3)
A 		38.4
(-3)
A 		97
C 50.3
(-3)
A 		123.2
(3)
A 		97.3
(0.9)
A 		76.3
(-0.9)
A 		73
avr 48.8
(-2.5) 		111.4
(2.3) 		121.1
(3) 		42.3
(-3) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 74.9
(-0.9)
A 		101.8
(3)
A 		69.7
(-1.7)
A 		79.9
(-0.2)
A 		92.6
(1.7)
A 		60.9
(-3)
A 		41
B 36.9
(-3)
A 		102.9
(3)
A 		92.7
(2.1)
A 		93
(2.1)
A 		81.3
(1)
A 		38.5
(-2.9)
A 		66
C 39.5
(-3)
A 		72
(1.5)
A 		82.4
(3)
A 		75.8
(2.1)
A 		81.2
(2.8)
A 		56.4
(-0.6)
A 		43
avr 50.4
(-3) 		92.2
(3) 		81.6
(1.5) 		82.9
(1.7) 		85
(2) 		51.9
(-2.8) 		42
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 34
1 2 3 4 range
A 17.4
(-0.1)
A 		19.9
(0.3)
A 		34.3
(3)
A 		1.8
(-3)
A 		33
B 15.4
(-1.1)
A 		29.5
(2.5)
A 		31.3
(3)
A 		8.2
(-3)
A 		23
C 16.7
(-2)
A 		35.4
(3)
A 		33.9
(2.6)
A 		12.9
(-3)
A 		23
avr 16.5
(-0.9) 		28.3
(1.9) 		33.2
(3) 		7.6
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 13.8
(-3)
A 		36.2
(3)
A 		15.2
(-2.6)
A 		20.3
(-1.3)
A 		27.9
(0.8)
A 		14.8
(-2.7)
A 		22
B 12.8
(-3)
A 		34.3
(3)
A 		22.5
(-0.3)
A 		24.6
(0.3)
A 		33
(2.6)
A 		14.4
(-2.6)
A 		22
C 14.6
(-3)
A 		22
(0.8)
A 		21
(0.3)
A 		15.2
(-2.7)
A 		26.3
(3)
A 		18.9
(-0.8)
A 		12
avr 13.7
(-3) 		30.8
(3) 		19.6
(-0.9) 		20
(-0.8) 		29.1
(2.4) 		16
(-2.2) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188477_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1040
Sequence Type Exemplar sequence
Representative Public ID ZK265.8 NCBI
Target Description ZK265.8 /REP_DB=WormBase Gene ID /WP=CE15303 /TR=Q93967 /GB=CAB00057.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=cuticulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059849(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059849 NCBI Caenorhabditis elegans ZK265.8 (cuticulin) mRNA, complete cds. 11/11 None
ZK265.8 ENSEMBL cdna:known chromosome:CEL140:I:8278520:8281722:1 gene:ZK265.8 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000040997 5/11 Cross Hyb Matching Probes None
SNAP00000040989 6/11 Cross Hyb Matching Probes None
GENEFINDER00000032258 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8278536-8281653(+) 100.0 100.0
Public Domain and Genome References
Gene Title cuticulin
Ensembl ZK265.8
Entrez Gene 3565740 Entrez gene
SwissProt Q93967 EMBL-EBI
WormBase CE15303 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB03520 Hypothetical protein ZK265.8 [Caenorhabditis elegans] emb|CAB00057.1| Hypothetical protein ZK265.8 [Caenorhabditis elegans] ref|NP_492250.1| ZK265.8 [Caenorhabditis elegans] 0.0
blast CAE60481 Hypothetical protein CBG04095 [Caenorhabditis briggsae] 0.0
blast CAA91480 Hypothetical protein T04F8.4 [Caenorhabditis elegans] ref|NP_509946.1| T04F8.4 [Caenorhabditis elegans] 5.0E-60
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 1.7E-63
Sequence
Target Sequence 
>C. ELEGANS:188477_AT
ggtattcgttattcatcagatcttcaacgtgcctacgcagaatcgtctgtattcaaattt
gctgataaacctggtgtatggtttttctgtcaagttcagatgtgtatgaagaaacatgga
atgtgtgatgggattacgcctccatcatgtggatcaatgtctcgagtaatatctgttgga
ggagaagataatggtggatttgaagaagaagaaaaggcgccttcgtctcgacgaaaaaca
acaccaaaaccatctggatcagattatgattatgagaatgaaaagccacaacagaaaact
acgaatgctccaagaagatccacaacagtttcaagggattatgattatgataacgaatca
ccaaaattacatagtccaaactctcattcttacaacagtgtgacacctccactcgactat
gagtcagttacattgactgctttctctcctccaattaatccagctactgtaggaatagag
agcgtatcgaaaactttcgaaacagaggacgacttggcttcttcttcagtgactccagag
ccaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTATTCGTTATTCATCAGATCTTC 648 505 709 Antisense
AACGTGCCTACGCAGAATCGTCTGT 676 143 734 Antisense
GGTGTATGGTTTTTCTGTCAAGTTC 298 497 781 Antisense
GGATTACGCCTCCATCATGTGGATC 354 509 839 Antisense
AGAAGAAAAGGCGCCTTCGTCTCGA 659 67 915 Antisense
TAGTCCAAACTCTCATTCTTACAAC 51 651 1080 Antisense
ACACCTCCACTCGACTATGAGTCAG 454 109 1111 Antisense
CAGTTACATTGACTGCTTTCTCTCC 410 207 1133 Antisense
CTCCTCCAATTAATCCAGCTACTGT 474 235 1154 Antisense
AACAGAGGACGACTTGGCTTCTTCT 636 135 1209 Antisense
CTTCTTCAGTGACTCCAGAGCCAAA 359 221 1229 Antisense
Sequence Source Affymetrix Proprietary Database