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[188472_s_at]

Description C52E12.2 /REP_DB=WormBase Gene ID /WP=CE06968 /GEN=unc-104 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=kinesin-like protein
Cluster 6grp_highest_10_10 > 41      6grp_high1_10_10 > 03      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 40      6grp_total > 24      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 24      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 433
1 2 3 4 range
A 790.9
(3)
P 		487.3
(-2.6)
P 		467.8
(-3)
P 		687.3
(1.1)
P 		323
B 854.1
(3)
P 		737.1
(1.4)
P 		421.2
(-3)
P 		699.9
(0.9)
P 		433
C 817.4
(3)
P 		654.4
(-0.6)
P 		545.6
(-3)
P 		737.5
(1.2)
P 		272
avr 820.8
(3) 		626.3
(-0.4) 		478.2
(-3) 		708.2
(1) 		343

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 573
1 2 3 4 5 6 range
A 830.6
(2.6)
P 		863
(3)
P 		561.6
(-0.8)
P 		538.6
(-1.1)
P 		414.4
(-2.6)
P 		385.6
(-3)
P 		477
B 836
(3)
P 		624.6
(0.4)
P 		524.5
(-0.9)
P 		449.1
(-1.8)
P 		354
(-3)
P 		389.1
(-2.6)
P 		482
C 912.8
(3)
P 		743.1
(1.2)
P 		760.2
(1.4)
P 		362.6
(-2.8)
P 		340.2
(-3)
P 		493.6
(-1.4)
P 		573
avr 859.8
(3) 		743.6
(1.6) 		615.4
(0) 		450.1
(-2) 		369.5
(-3) 		422.8
(-2.3) 		490
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 190
1 2 3 4 range
A 288
(3)
P 		105.8
(-2.8)
P 		122.3
(-2.3)
P 		100.6
(-3)
P 		187
B 272.4
(3)
P 		182.1
(-0.1)
P 		97.6
(-3)
P 		149.2
(-1.2)
P 		175
C 270.6
(3)
P 		188.2
(-0.4)
P 		190.1
(-0.3)
P 		124.4
(-3)
P 		146
avr 277
(3) 		158.7
(-1.7) 		136.7
(-2.5) 		124.7
(-3) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 265
1 2 3 4 5 6 range
A 153.1
(-1.3)
P 		306.9
(3)
P 		122.3
(-2.2)
P 		136.6
(-1.8)
P 		125.1
(-2.1)
P 		93.9
(-3)
P 		213
B 290.9
(3)
P 		207.9
(0.1)
P 		127.4
(-2.7)
P 		119
(-3)
P 		143.8
(-2.1)
P 		145.1
(-2.1)
P 		172
C 338.3
(3)
P 		226.8
(0.5)
P 		193.5
(-0.3)
P 		72.9
(-3)
P 		110.1
(-2.2)
P 		165.2
(-0.9)
P 		265
avr 260.8
(3) 		247.2
(2.5) 		147.7
(-1.5) 		109.5
(-3) 		126.3
(-2.3) 		134.7
(-2) 		151
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188472_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5085
Sequence Type Exemplar sequence
Representative Public ID C52E12.2 NCBI
Target Description C52E12.2 /REP_DB=WormBase Gene ID /WP=CE06968 /GEN=unc-104 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=kinesin-like protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171017(11), NM_001026870(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171017 NCBI Caenorhabditis elegans UNCoordinated family member (unc-104) (unc-104) mRNA, complete cds. 11/11 A 
NM_001026870 NCBI Caenorhabditis elegans UNCoordinated family member (unc-104) (unc-104) mRNA, complete cds. 11/11 None
SNAP00000036563 ENSEMBL cdna:SNAP chromosome:CEL140:II:7005923:7010140:1 11/11 None
GENEFINDER00000036572 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7005011:7010140:1 11/11 None
C52E12.2a.2 ENSEMBL cdna:known chromosome:CEL140:II:6994982:7010537:1 gene:C52E12.2 11/11 A 
C52E12.2a.1 ENSEMBL cdna:known chromosome:CEL140:II:6994982:7010547:1 gene:C52E12.2 11/11 A 
C52E12.2b ENSEMBL cdna:known chromosome:CEL140:II:6995865:7010140:1 gene:C52E12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6995860-7010136(+) 100.0 100.0
Public Domain and Genome References
Ensembl C52E12.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM98043 Uncoordinated protein 104, isoform a [Caenorhabditis elegans] ref|NP_741019.2| UNCoordinated family member (unc-104) [Caenorhabditis elegans] sp|P23678|UN104_CAEEL Kinesin-like protein unc-104 (Uncoordinated protein 104) 0.0
blast T15822 kinesin-like protein unc-104 - Caenorhabditis elegans 0.0
blast JN0114 kinesin-related protein unc-104 - Caenorhabditis elegans 0.0
blast AAM98044 Uncoordinated protein 104, isoform b [Caenorhabditis elegans] ref|NP_001022041.1| UNCoordinated family member (unc-104) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.7.Methicillin resistance protein FemA probable tRNA-binding arm All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Methicillin resistance protein FemA probable tRNA-binding arm 1.20000004768372
scop a.2.7.Methicillin resistance protein FemA probable tRNA-binding arm All alpha proteins; Long alpha-hairpin; tRNA-binding arm; Methicillin resistance protein FemA probable tRNA-binding arm 1.10000002384186
Pfam IPR000253 EMBL-EBI Forkhead-associated (FHA) 2.5E-13
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.6E-19
Pfam IPR001752 EMBL-EBI Kinesin, motor region 1.0E-126
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.6E-19
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.6E-19
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.6E-19
Sequence
Target Sequence 
>C. ELEGANS:188472_S_AT
agtataacatctccagtgtctgatagatccttgatcaaatcctctcgatcttcggatctc
ctgtgccgacagaaatcaaaatccgatcagaaccttgcatcaaatgatgatatagttgat
aatttaggaggaatgaaacgaagtttgagtggatcacgaattcttcaactcaatatttta
gtgccagaagtacttgaagaaagagttggagttgttgtttcaaagaaaggatatatgaat
ttcttggaagaaaagactcaaggatggactcgtcgatgggttattgttcgtcgtccatat
attcttttattccgtgatgatcgagatttggttattcgaggaatcattaatctggcaaat
gctagaattgaacattcggaagatcaacaagcaatggtgaaagtaccaaatactttcagt
gtttgcactaatcaacgtggattccttatgcaaatgatgccgggcgatgagatgtatgat
tggctgtacgcaatcaatccattg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTATAACATCTCCAGTGTCTGATA 326 63 4177 Antisense
GATCAAATCCTCTCGATCTTCGGAT 407 421 4209 Antisense
TTCGGATCTCCTGTGCCGACAGAAA 396 695 4227 Antisense
AAATCCGATCAGAACCTTGCATCAA 482 119 4255 Antisense
GTGGATCACGAATTCTTCAACTCAA 56 491 4325 Antisense
GACTCAAGGATGGACTCGTCGATGG 499 373 4431 Antisense
GTCGATGGGTTATTGTTCGTCGTCC 104 469 4448 Antisense
TGTTCGTCGTCCATATATTCTTTTA 127 563 4461 Antisense
ATATTCTTTTATTCCGTGATGATCG 306 19 4475 Antisense
ATGATGCCGGGCGATGAGATGTATG 658 43 4630 Antisense
TTGGCTGTACGCAATCAATCCATTG 369 707 4656 Antisense
Sequence Source Affymetrix Proprietary Database