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[188468_at]

Description F32A6.5 /REP_DB=WormBase Gene ID /WP=CE04473 /GEN=sto-2 /TR=SW:Q19958 /GB=AAA81390.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 24      4grp_high_8_8 > 26      6grp_highest_nonorm > 90      6grp_total > 02      4grp_total > 30      6grp_highest_sub > 02      6grp_cgc > 52      6grp_high1_sub > 02      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 133.3
(1)
P 		117.4
(-0.1)
P 		76.8
(-3)
M 		161.1
(3)
P 		84
B 131.5
(3)
P 		101.7
(-3)
P 		121.4
(1)
P 		113.7
(-0.6)
P 		30
C 131.8
(3)
P 		83.4
(-0.2)
P 		41.1
(-3)
P 		56.1
(-2)
P 		91
avr 132.2
(3) 		100.8
(-0.6) 		79.8
(-3) 		110.3
(0.5) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 128
1 2 3 4 5 6 range
A 95.7
(-0.5)
P 		131.3
(3)
P 		94.3
(-0.6)
P 		70.4
(-3)
P 		124.6
(2.3)
P 		71.7
(-2.9)
P 		61
B 179.4
(2.7)
P 		185.9
(3)
P 		116.3
(-0.3)
P 		117.3
(-0.2)
P 		118.7
(-0.2)
P 		58.4
(-3)
P 		128
C 98.6
(-1.1)
P 		181.9
(3)
P 		60.7
(-2.9)
P 		143.4
(1.1)
P 		59
(-3)
P 		91.3
(-1.4)
P 		123
avr 124.6
(0.3) 		166.4
(3) 		90.4
(-1.9) 		110.4
(-0.6) 		100.8
(-1.3) 		73.8
(-3) 		93
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 39
1 2 3 4 range
A 48.5
(3)
P 		25.5
(-1.9)
P 		20.1
(-3)
M 		23.6
(-2.3)
P 		28
B 41.9
(3)
P 		25.1
(-2.7)
P 		28.1
(-1.7)
P 		24.2
(-3)
P 		18
C 43.6
(3)
P 		24
(-0.4)
P 		14.3
(-2.2)
P 		9.5
(-3)
P 		34
avr 44.7
(3) 		24.9
(-1.6) 		20.8
(-2.6) 		19.1
(-3) 		26

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 17.6
(-3)
P 		46.7
(3)
P 		20.5
(-2.4)
P 		17.9
(-2.9)
P 		37.6
(1.1)
P 		17.5
(-3)
P 		29
B 62.4
(3)
P 		61.9
(2.9)
P 		28.2
(-2.1)
P 		31.1
(-1.6)
P 		48.2
(0.9)
P 		21.8
(-3)
P 		41
C 36.5
(0.2)
P 		55.5
(3)
P 		15.5
(-3)
P 		28.8
(-1)
P 		19.1
(-2.5)
P 		30.6
(-0.7)
P 		40
avr 38.8
(0.1) 		54.7
(3) 		21.4
(-3) 		25.9
(-2.2) 		35
(-0.5) 		23.3
(-2.7) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188468_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16434
Sequence Type Exemplar sequence
Representative Public ID F32A6.5 NCBI
Target Description F32A6.5 /REP_DB=WormBase Gene ID /WP=CE04473 /GEN=sto-2 /TR=SW:Q19958 /GB=AAA81390.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076501(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076501 NCBI Caenorhabditis elegans STOmatin family member (sto-2) (sto-2) mRNA, complete cds. 11/11 None
GENEFINDER00000030569 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:5307797:5311728:-1 11/11 None
SNAP00000030568 ENSEMBL cdna:SNAP chromosome:CEL140:X:5307928:5309226:-1 11/11 None
F32A6.5 ENSEMBL cdna:known chromosome:CEL140:X:5307523:5311728:-1 gene:F32A6.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:5307813-5309488(-) 98.1 98.1
Public Domain and Genome References
Gene Title
Gene Symbol sto-2
Ensembl F32A6.5
Entrez Gene 180802 Entrez gene
SwissProt Q19958 EMBL-EBI
WormBase CE33065 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.9982.1.S1_AT similar to stomatin-like 3 cfa
CHICKEN:GGAAFFX.10913.1.S1_AT similar to stomatin-like 3; erythrocyte band 7 integral membrane protein; protein 7.2B gga
U133_X3P:HS2.327794.1.S1_3P_AT stomatin (EPB72)-like 3 hs
HG-U133_PLUS_2:1553794_AT stomatin (EPB72)-like 3 hs
RG-U34B:RC_AI012390_AT stomatin (Epb7.2)-like 3 (predicted) rn
RAE230B:1397148_AT stomatin (Epb7.2)-like 3 (predicted) rn
RAT230_2:1397148_AT stomatin (Epb7.2)-like 3 (predicted) rn
RAE230B:1383508_AT stomatin (Epb7.2)-like 3 (predicted) rn
RAT230_2:1383508_AT stomatin (Epb7.2)-like 3 (predicted) rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81390 Stomatin protein 2 [Caenorhabditis elegans] ref|NP_508902.3| STOmatin family member (sto-2) [Caenorhabditis elegans] sp|Q19958|STO2_CAEEL Stomatin protein 1.0E-166
blast CAE68730 Hypothetical protein CBG14656 [Caenorhabditis briggsae] 1.0E-154
blast AAA81390 Stomatin protein 2 [Caenorhabditis elegans] ref|NP_508902.3| STOmatin family member (sto-2) [Caenorhabditis elegans] sp|Q19958|STO2_CAEEL Stomatin protein 1.0E-154
blast T16240 hypothetical protein F32A6.5 - Caenorhabditis elegans 1.0E-131
blast T16240 hypothetical protein F32A6.5 - Caenorhabditis elegans 1.0E-121
blast AAA81390 Stomatin protein 2 [Caenorhabditis elegans] ref|NP_508902.3| STOmatin family member (sto-2) [Caenorhabditis elegans] sp|Q19958|STO2_CAEEL Stomatin protein 1.0E-110
Protein Domains
Database ID Description E-Value
Pfam IPR001107 EMBL-EBI Band 7 protein 8.0E-80
Pfam IPR001107 EMBL-EBI Band 7 protein 8.0E-80
Pfam IPR001107 EMBL-EBI Band 7 protein 8.0E-80
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_508902.3 1 70-92
F32A6.5 1 70-92
GENEFINDER00000030569 1 94-116
Sequence
Target Sequence 
>C. ELEGANS:188468_AT
gacttggtcgactgattggtggtggagccaaaggaccaggaattttctttgtacttccgt
gcatagaatcgtacaccaaggttgatctgagaacagtatcgtttagtgtgccgccgcagg
aaattttaacaaaagattcagtgacaacgtctgtcgacgctgttatttactataggatca
gcaatgcaacagttagcgtggcaaatgttgaaaatgcacaccattctacaagattgcttg
cgcaaacaacgcttcgtaatatgttaggtacgagaagcctgtcagaaattctctcggatc
gtgaaacacttgcagcatccatgcagacaattcttgatgaagcaactgaaagctggggaa
tcaaagtggaaagagtagaaatcaaagatgttcgtctcccaattcaacttcaacgagcaa
tggcggcagaagcagaggccactcgagaagctagagctaaggtgattgctgcggaaggag
agcagaaagcttctcgggcattgcgtgatgctgcatcagtcattgctcagtctccagctg
ctcttcaactccgatatcttcaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GACTTGGTCGACTGATTGGTGGTGG 684 365 227 Antisense
ATTTTCTTTGTACTTCCGTGCATAG 175 15 268 Antisense
GAACAGTATCGTTTAGTGTGCCGCC 415 349 317 Antisense
GTCTGTCGACGCTGTTATTTACTAT 438 471 375 Antisense
ATTGCTTGCGCAAACAACGCTTCGT 695 5 459 Antisense
GAAGCCTGTCAGAAATTCTCTCGGA 358 345 500 Antisense
TTCGTCTCCCAATTCAACTTCAACG 382 695 617 Antisense
GCGGCAGAAGCAGAGGCCACTCGAG 101 295 649 Antisense
CAGAAAGCTTCTCGGGCATTGCGTG 301 205 709 Antisense
GCTGCATCAGTCATTGCTCAGTCTC 251 297 736 Antisense
GCTCTTCAACTCCGATATCTTCAAA 272 301 766 Antisense
Sequence Source Affymetrix Proprietary Database