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[188457_at]

Description M04D8.1 /REP_DB=WormBase Gene ID /WP=CE00576 /GEN=ins-21 /TR=SW:Q21507 /GB=CAA83610.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Cluster 4grp_highest_8_8 > 30      4grp_high_8_8 > 04      6grp_total > 10      4grp_total > 05      6grp_cgc > 65      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 134.9
(0.1)
P 		83.3
(-3)
P 		121.4
(-0.7)
P 		183.8
(3)
P 		101
B 149.8
(0.3)
P 		68.6
(-3)
P 		121.2
(-0.9)
P 		216.4
(3)
P 		148
C 96.1
(-2.5)
P 		96.7
(-2.5)
P 		83.4
(-3)
P 		234.1
(3)
P 		151
avr 126.9
(-0.9) 		82.9
(-3) 		108.7
(-1.8) 		211.4
(3) 		129

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 126
1 2 3 4 5 6 range
A 5.6
(-3)
A 		69.7
(0.1)
P 		129.5
(3)
P 		86.7
(0.9)
M 		56.3
(-0.5)
A 		92.9
(1.2)
P 		124
B 50.3
(-3)
A 		131.1
(3)
P 		65.8
(-1.8)
A 		74.6
(-1.2)
P 		81.6
(-0.7)
P 		59.7
(-2.3)
P 		81
C 54.5
(-2)
A 		98.5
(3)
P 		97.2
(2.9)
P 		62.8
(-1.1)
A 		46.1
(-3)
P 		87.7
(1.8)
P 		52
avr 36.8
(-3) 		99.8
(3) 		97.5
(2.8) 		74.7
(0.6) 		61.3
(-0.7) 		80.1
(1.1) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 32
1 2 3 4 range
A 49.1
(3)
P 		18.1
(-3)
P 		31.7
(-0.4)
P 		26.9
(-1.3)
P 		31
B 47.8
(3)
P 		16.9
(-3)
P 		28.1
(-0.8)
P 		46.1
(2.7)
P 		31
C 31.8
(-0.9)
P 		27.8
(-3)
P 		29.1
(-2.3)
P 		39.5
(3)
P 		12
avr 42.9
(3) 		20.9
(-3) 		29.6
(-0.6) 		37.5
(1.5) 		22

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 1
(-3)
A 		24.8
(2.3)
P 		28.2
(3)
P 		22
(1.6)
M 		17
(0.5)
A 		22.6
(1.8)
P 		27
B 17.5
(-2.7)
A 		43.7
(3)
P 		16
(-3)
A 		19.8
(-2.2)
P 		33.2
(0.7)
P 		22.3
(-1.6)
P 		28
C 20.2
(-0.4)
A 		30.1
(3)
P 		24.7
(1.1)
P 		12.6
(-3)
A 		14.9
(-2.2)
P 		29.3
(2.7)
P 		18
avr 12.9
(-3) 		32.9
(3) 		23
(0) 		18.1
(-1.4) 		21.7
(-0.4) 		24.7
(0.5) 		20
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188457_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7768
Sequence Type Exemplar sequence
Representative Public ID M04D8.1 NCBI
Target Description M04D8.1 /REP_DB=WormBase Gene ID /WP=CE00576 /GEN=ins-21 /TR=SW:Q21507 /GB=CAA83610.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066821(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066821 NCBI Caenorhabditis elegans INSulin related family member (ins-21) (ins-21) mRNA, complete cds. 10/11 None
M04D8.1 ENSEMBL cdna:known chromosome:CEL140:III:10023695:10024011:1 gene:M04D8.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10023690-10024007(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-21
Ensembl M04D8.1
Entrez Gene 191689 Entrez gene
WormBase CE35999 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA83610 Hypothetical protein M04D8.1 [Caenorhabditis elegans] ref|NP_499222.2| INSulin related family member (ins-21) [Caenorhabditis elegans] 2.0E-23
blast Q21507 Probable insulin-like peptide alpha-type 1 precursor 3.0E-20
blast CAE65066 Hypothetical protein CBG09916 [Caenorhabditis briggsae] 5.0E-16
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_499222.2 1 4-20
M04D8.1 1 4-20
Sequence
Target Sequence 
>C. ELEGANS:188457_AT
gaaaacctactcatttttcgtgctttttattgtattcatcttttttatttcttcatcaaa
atctcattcaaagaaacatgttcgtttcctttgtgcaacaaaagcggtcaaacacattcg
gaaagtatgccctgatatgtgtctcactggaggtaagattgaaaagtttcccgtttttta
tccaaattttaaagaagtcgaagtcaatgagttttgcaagatggggtactcggattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAAACCTACTCATTTTTCGTGCTT 189 355 15 Antisense
CATTTTTCGTGCTTTTTATTGTATT 624 215 26 Antisense
TTTTATTGTATTCATCTTTTTTATT 388 675 39 Antisense
AACATGTTCGTTTCCTTTGTGCAAC 28 135 89 Antisense
TGTTCGTTTCCTTTGTGCAACAAAA 363 561 93 Antisense
GCAACAAAAGCGGTCAAACACATTC 284 319 109 Antisense
AACACATTCGGAAAGTATGCCCTGA 310 135 125 Antisense
AGTATGCCCTGATATGTGTCTCACT 320 63 138 Antisense
GTGTCTCACTGGAGGTAAGATTGAA 645 483 153 Antisense
GAAGTCGAAGTCAATGAGTTTTGCA 111 337 208 Antisense
TTTGCAAGATGGGGTACTCGGATTC 552 665 227 Antisense
Sequence Source Affymetrix Proprietary Database