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[188455_at]

Description ZK1307.5 /REP_DB=WormBase Gene ID /WP=CE01694 /GEN=sqv-8 /TR=SW:Q09363 /GB=CAA87436.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 0      6grp_high1_10_10 > 08      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 73      6grp_total > 09      4grp_total > 20      6grp_cgc > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 352
1 2 3 4 range
A 565
(-0.7)
P 		482.4
(-2.2)
P 		435.2
(-3)
P 		778.4
(3)
P 		343
B 560.3
(-0.5)
P 		511.1
(-1.4)
P 		426.8
(-3)
P 		746.2
(3)
P 		319
C 613.5
(0.5)
P 		575.7
(-0.3)
P 		434.3
(-3)
P 		743.1
(3)
P 		309
avr 579.6
(-0.3) 		523.1
(-1.3) 		432.1
(-3) 		755.9
(3) 		324

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 263
1 2 3 4 5 6 range
A 654
(3)
P 		476.5
(-1.1)
P 		510.2
(-0.3)
P 		514.9
(-0.2)
P 		451.7
(-1.6)
P 		391.4
(-3)
P 		263
B 652.4
(3)
P 		524.8
(-0.6)
P 		439.5
(-3)
P 		572
(0.7)
P 		619.6
(2.1)
P 		508.5
(-1.1)
P 		213
C 529.9
(0.1)
P 		574.4
(3)
P 		572
(2.8)
P 		563
(2.3)
P 		481.8
(-3)
P 		488.4
(-2.6)
P 		93
avr 612.1
(3) 		525.2
(-0.5) 		507.2
(-1.2) 		550
(0.5) 		517.7
(-0.8) 		462.8
(-3) 		149
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 107
1 2 3 4 range
A 205.7
(3)
P 		104.7
(-3)
P 		113.8
(-2.5)
P 		113.9
(-2.5)
P 		101
B 178.7
(3)
P 		126.2
(-0.9)
P 		98.9
(-3)
P 		159
(1.5)
P 		80
C 203.1
(3)
P 		165.5
(0.1)
P 		151.4
(-1)
P 		125.3
(-3)
P 		78
avr 195.8
(3) 		132.1
(-2.1) 		121.4
(-3) 		132.7
(-2.1) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 156
1 2 3 4 5 6 range
A 120.6
(-1)
P 		169.5
(3)
P 		111.1
(-1.7)
P 		130.6
(-0.1)
P 		136.3
(0.3)
P 		95.3
(-3)
P 		74
B 227
(2)
P 		174.7
(-0.2)
P 		106.7
(-3)
P 		151.5
(-1.1)
P 		251.7
(3)
P 		189.6
(0.4)
P 		145
C 196.4
(3)
P 		175.3
(1.5)
P 		145.6
(-0.7)
P 		113.2
(-3)
P 		155.9
(0.1)
P 		163.5
(0.6)
P 		83
avr 181.3
(3) 		173.2
(2.2) 		121.1
(-3) 		131.8
(-1.9) 		181.3
(3) 		149.5
(-0.2) 		60
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188455_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3896
Sequence Type Exemplar sequence
Representative Public ID ZK1307.5 NCBI
Target Description ZK1307.5 /REP_DB=WormBase Gene ID /WP=CE01694 /GEN=sqv-8 /TR=SW:Q09363 /GB=CAA87436.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063675(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063675 NCBI Caenorhabditis elegans SQuashed Vulva family member (sqv-8) (sqv-8) mRNA, complete cds. 11/11 None
SNAP00000021423 ENSEMBL cdna:SNAP chromosome:CEL140:II:9647849:9649135:1 11/11 None
GENEFINDER00000021435 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9647849:9649135:1 11/11 None
ZK1307.5 ENSEMBL cdna:known chromosome:CEL140:II:9647800:9649278:1 gene:ZK1307.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9647845-9649132(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol sqv-8
Ensembl ZK1307.5
Entrez Gene 174517 Entrez gene
SwissProt Q09363 EMBL-EBI
WormBase CE01694 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.4503.1.S1_AT similar to beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) cfa
CANINE_2:CFAAFFX.24190.1.S1_AT similar to beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) cfa
HU35KSUBB:RC_F02383_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U133A:203452_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U133_PLUS_2:203452_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U133A_2:203452_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
U133_X3P:G12408653_3P_S_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U133A_2:35179_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-FOCUS:35179_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U133_PLUS_2:35179_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U133A:35179_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U95AV2:35179_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
HG-U95D:88835_AT Beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) hs
MU11KSUBA:AA254626_F_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MG-U74AV2:94407_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MG-U74BV2:164595_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MOE430A:1431219_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MOUSE430A_2:1431219_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MOUSE430_2:1431219_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MOE430A:1416960_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MOUSE430A_2:1416960_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MOUSE430_2:1416960_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) mm
MU19KSUBA:TC23867_S_AT Beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I), mRNA (cDNA clone MGC:21378 IMAGE:4500993) mm
MU19KSUBB:TC32192_AT Beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I), mRNA (cDNA clone MGC:21378 IMAGE:4500993) mm
MU19KSUBB:TC32192_G_AT Beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I), mRNA (cDNA clone MGC:21378 IMAGE:4500993) mm
RG-U34C:RC_AI176245_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (predicted) rn
RAE230A:1373014_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (predicted) rn
RAT230_2:1373014_AT beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) (predicted) rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
15018 galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87436 Hypothetical protein ZK1307.5 [Caenorhabditis elegans] emb|CAA06741.1| Sqv-8 protein [Caenorhabditis elegans] ref|NP_496076.1| SQuashed Vulva family member (sqv-8) [Caenorhabditis elegans] sp|Q09363|SQV8_CAEEL Probable glucuronosyltransferase sqv-8 (Vulval invagination protein sqv-8) (Squashed vulva protein 8) 0.0
blast CAI63871 beta3-glucuronyltransferase [Caenorhabditis briggsae] emb|CAE58002.1| Hypothetical protein CBG01067 [Caenorhabditis briggsae] 1.0E-172
blast XP_396716 PREDICTED: similar to glucuronyltransferase I [Apis mellifera] 2.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR005027 EMBL-EBI Glycosyl transferase, family 43 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496076.1 1 10-30
ZK1307.5 1 10-30
SNAP00000021423 1 10-30
GENEFINDER00000021435 1 10-30
Sequence
Target Sequence 
>C. ELEGANS:188455_AT
taaatgctcgaactccgtctgatcaaaaaatgagatacgatgaccccaattggacgttgc
cacgtggcgttgagcaacgaaatcgagcacttttatggattcaaaatcaattgagtggtg
tgaaagagggcgttgtgtactttggagatgatgataacacatatgatctgaaaattttcg
gagaaatgcggaaagtgaaaaatgcaggagtttggccagttggaatagttggtggaatgt
ttgtagaaacgccaattttagagaaaaatggatcgatttcccatttcaacgctgtctgga
aaccggagcgtccatttccgatcgatatggctgcatttgcagtcaatatttctctggttc
tctccaacgcgaacgctcttttctcattcgacgtgcctcgtggatatcaagaatctactt
ttcttgaaaatcttggaattcatcgttataatatggaaccacttgcggaaatgtgcacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAAATGCTCGAACTCCGTCTGATCA 260 639 482 Antisense
CGATGACCCCAATTGGACGTTGCCA 463 203 519 Antisense
GAGGGCGTTGTGTACTTTGGAGATG 27 403 607 Antisense
AAATGCAGGAGTTTGGCCAGTTGGA 654 119 681 Antisense
TGGATCGATTTCCCATTTCAACGCT 662 549 750 Antisense
TTCAACGCTGTCTGGAAACCGGAGC 218 677 766 Antisense
TGGAAACCGGAGCGTCCATTTCCGA 429 547 778 Antisense
ATTTCCGATCGATATGGCTGCATTT 6 17 795 Antisense
CTCATTCGACGTGCCTCGTGGATAT 356 227 864 Antisense
GACGTGCCTCGTGGATATCAAGAAT 13 379 871 Antisense
GGAACCACTTGCGGAAATGTGCACG 350 529 936 Antisense
Sequence Source Affymetrix Proprietary Database