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[188452_s_at]

Description B0523.5 /REP_DB=WormBase Gene ID /WP=CE06721 /GEN=sup-5 /TR=SW:P34268 /GB=AAB37088.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 43      6grp_high1_10_10 > 34      4grp_highest_8_8 > 51      4grp_high_8_8 > 32      6grp_highest_nonorm > 90      6grp_total > 02      4grp_total > 47      6grp_highest_sub > 02      6grp_cgc > 53      6grp_high1_sub > 02      6grp_cgc_sub > 02      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 183.8
(3)
P 		121.9
(-1.2)
P 		157.6
(1.2)
P 		96.2
(-3)
P 		88
B 167.2
(3)
P 		120.8
(-2.2)
P 		113.3
(-3)
P 		133.9
(-0.7)
P 		54
C 180
(3)
P 		121.3
(-1.4)
P 		100.3
(-3)
P 		135.9
(-0.3)
P 		80
avr 177
(3) 		121.3
(-3) 		123.7
(-2.7) 		122
(-2.9) 		56

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 132
1 2 3 4 5 6 range
A 113.4
(-3)
P 		181.1
(1.4)
P 		128.8
(-2)
P 		204.7
(3)
P 		141.2
(-1.2)
P 		172.7
(0.9)
P 		91
B 146.6
(-2)
P 		219.5
(3)
P 		141.5
(-2.3)
P 		172.7
(-0.2)
P 		147.9
(-1.9)
P 		132
(-3)
P 		88
C 235.1
(2.4)
P 		244.9
(3)
P 		199.1
(0.4)
P 		138.2
(-3)
P 		155.7
(-2)
P 		162.8
(-1.6)
P 		107
avr 165
(-1.5) 		215.2
(3) 		156.5
(-2.3) 		171.9
(-0.9) 		148.3
(-3) 		155.8
(-2.3) 		67
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 53
1 2 3 4 range
A 66.9
(3)
P 		26.5
(-1.6)
P 		41.2
(0.1)
P 		14.1
(-3)
P 		53
B 53.3
(3)
P 		29.8
(-2.2)
P 		26.3
(-3)
P 		28.5
(-2.5)
P 		27
C 59.6
(3)
P 		34.9
(-1)
P 		35
(-1)
P 		22.9
(-3)
P 		37
avr 59.9
(3) 		30.4
(-1.6) 		34.2
(-1) 		21.8
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 66
1 2 3 4 5 6 range
A 20.9
(-3)
P 		64.4
(3)
P 		28
(-2)
P 		51.9
(1.3)
P 		42.6
(-0)
P 		42
(-0.1)
P 		44
B 51
(-0.4)
P 		73.1
(3)
P 		34.4
(-3)
P 		45.7
(-1.2)
P 		60.1
(1)
P 		49.2
(-0.7)
P 		39
C 87.1
(3)
P 		74.7
(1.7)
P 		50.7
(-0.7)
P 		27.8
(-3)
P 		50.4
(-0.7)
P 		54.5
(-0.3)
P 		59
avr 53
(-0.2) 		70.7
(3) 		37.7
(-3) 		41.8
(-2.3) 		51
(-0.6) 		48.6
(-1) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 188452_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7457
Sequence Type Exemplar sequence
Representative Public ID B0523.5 NCBI
Target Description B0523.5 /REP_DB=WormBase Gene ID /WP=CE06721 /GEN=sup-5 /TR=SW:P34268 /GB=AAB37088.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066512(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066512 NCBI Caenorhabditis elegans FLI-I (Drosophila flightless) homolog family member (fli-1) (fli-1) mRNA, complete cds. 11/11 A 
B0523.5 ENSEMBL cdna:known chromosome:CEL140:III:8675225:8683977:-1 gene:B0523.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8675991-8683590(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol fli-1
Ensembl B0523.5
Entrez Gene 176215 Entrez gene
SwissProt P34268 EMBL-EBI
WormBase CE36522 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1640391_AT flightless I dm
DROSGENOME1:143161_AT flightless I dm
CHICKEN:GGAAFFX.11777.1.S1_AT flightless I homolog (Drosophila) gga
CHICKEN:GGAAFFX.3112.1.S1_S_AT flightless I homolog (Drosophila) gga
HG-U133_PLUS_2:212024_X_AT flightless I homolog (Drosophila) hs
HG-U133A:212024_X_AT flightless I homolog (Drosophila) hs
HG-U133A_2:212024_X_AT flightless I homolog (Drosophila) hs
HG-U133_PLUS_2:212025_S_AT flightless I homolog (Drosophila) hs
HG-U133A_2:212025_S_AT flightless I homolog (Drosophila) hs
HG-U133A:212025_S_AT flightless I homolog (Drosophila) hs
HUGENEFL:U80184_RNA1_AT flightless I homolog (Drosophila) hs
U133_X3P:HS.83849.2.S2_3P_A_AT flightless I homolog (Drosophila) hs
U133_X3P:HS.83849.2.S1_3P_A_AT flightless I homolog (Drosophila) hs
U133_X3P:HS.83849.1.A1_3P_A_AT flightless I homolog (Drosophila) hs
HG-U133A_2:222065_S_AT flightless I homolog (Drosophila) hs
HG-FOCUS:222065_S_AT flightless I homolog (Drosophila) hs
HG-U133_PLUS_2:222065_S_AT flightless I homolog (Drosophila) hs
HG-U133A:222065_S_AT flightless I homolog (Drosophila) hs
HG-U133_PLUS_2:227302_S_AT flightless I homolog (Drosophila) hs
HG-U133B:227302_S_AT flightless I homolog (Drosophila) hs
HG-U95AV2:33133_AT flightless I homolog (Drosophila) hs
HG-U95E:88279_S_AT flightless I homolog (Drosophila) hs
HG-U95E:88281_S_AT flightless I homolog (Drosophila) hs
MU11KSUBA:C79694_RC_S_AT flightless I homolog (Drosophila) mm
MU11KSUBA:AA038461_AT flightless I homolog (Drosophila) mm
MU11KSUBA:AA038461_G_AT flightless I homolog (Drosophila) mm
MG-U74AV2:95489_AT flightless I homolog (Drosophila) mm
MG-U74AV2:97593_F_AT flightless I homolog (Drosophila) mm
MG-U74AV2:97594_R_AT flightless I homolog (Drosophila) mm
MOE430A:1439356_AT flightless I homolog (Drosophila) mm
MOUSE430A_2:1439356_AT flightless I homolog (Drosophila) mm
MOUSE430_2:1439356_AT flightless I homolog (Drosophila) mm
MOE430A:1416044_AT flightless I homolog (Drosophila) mm
MOUSE430A_2:1416044_AT flightless I homolog (Drosophila) mm
MOUSE430_2:1416044_AT flightless I homolog (Drosophila) mm
MOE430A:1448189_A_AT flightless I homolog (Drosophila) mm
MOUSE430A_2:1448189_A_AT flightless I homolog (Drosophila) mm
MOUSE430_2:1448189_A_AT flightless I homolog (Drosophila) mm
MU19KSUBC:TC40240_AT Flightless I homolog (Drosophila), mRNA (cDNA clone IMAGE:4161799) mm
MU19KSUBC:TC41738_AT Flightless I homolog (Drosophila), mRNA (cDNA clone IMAGE:4161799) mm
RG-U34B:RC_AA850662_AT flightless I homolog (Drosophila) (predicted) rn
RAE230A:1372113_AT flightless I homolog (Drosophila) (predicted) rn
RAT230_2:1372113_AT flightless I homolog (Drosophila) (predicted) rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
3779 actin binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB37088 Fli-i (drosophila flightless) homolog protein 1 [Caenorhabditis elegans] ref|NP_498913.2| FLI-I (Drosophila flightless) homolog family member (fli-1) [Caenorhabditis elegans] sp|P34268|FLII_CAEEL Flightless-I protein homolog gb|AAC03567.1| flightless-I homolog [Caenorhabditis elegans] 0.0
blast CAE57608 Hypothetical protein CBG00589 [Caenorhabditis briggsae] 0.0
blast S44732 b0523.5 protein - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.016
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.051
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.036
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.013
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.38
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.072
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.057
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 8.8E-5
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.23
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.011
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.011
Pfam IPR007123 EMBL-EBI Gelsolin region 1.5E-18
Pfam IPR007123 EMBL-EBI Gelsolin region 3.9E-19
Pfam IPR007123 EMBL-EBI Gelsolin region 1.2E-19
Pfam IPR007123 EMBL-EBI Gelsolin region 3.4E-16
Sequence
Target Sequence 
>C. ELEGANS:188452_S_AT
tgctcagcattctgtcacatgctccgaattccattcaaagaaatcgaagaagatggtcat
cgtggagtggtttacgtatggatgggtaaagattctgatccacgtgaacatgaatttgct
cgacaagtagcatcagatcttgtcgttcgtgacgatgacaatgacttccgaattgtcgaa
gttcaagaaggagaagaaaatgaagaattctggaaagttctcggtggaaagaaaaaatat
gaaactgatagttcatttgttaagcatacaagactgtttcgatgcaccaacgagaaggga
tactttgcgatttccgagaaaactgtcgatttttgtcaggatgatcttgatgatgacgat
ataatgattctggataacggagatgcagttttcctttggattggagcaagatcttcagat
attgaagcaaaactttcttatcaagccgctcaagtatatcatgcatcaatgagaatgaaa
gcaaatgagaagccgagaaagttcatgttagcagttcgaggacgagaaagttgcagattc
cggaaatgcttccatgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTCAGCATTCTGTCACATGCTCC 350 581 3199 Antisense
TCACATGCTCCGAATTCCATTCAAA 439 627 3213 Antisense
AGATGGTCATCGTGGAGTGGTTTAC 143 69 3249 Antisense
GTAGCATCAGATCTTGTCGTTCGTG 462 457 3325 Antisense
GTGACGATGACAATGACTTCCGAAT 41 483 3347 Antisense
ACAAGACTGTTTCGATGCACCAACG 6 115 3466 Antisense
GATACTTTGCGATTTCCGAGAAAAC 385 423 3497 Antisense
GGAGATGCAGTTTTCCTTTGGATTG 321 519 3577 Antisense
GCAAAACTTTCTTATCAAGCCGCTC 24 319 3625 Antisense
AAGCCGCTCAAGTATATCATGCATC 304 149 3641 Antisense
GCAGATTCCGGAAATGCTTCCATGC 7 311 3731 Antisense
Sequence Source Affymetrix Proprietary Database