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[188448_at] calponin

Description D1069.2 /REP_DB=WormBase Gene ID /WP=CE17614 /TR=O44788 /GB=AAB94947.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=calponin
Cluster 6grp_highest_10_10 > 07      6grp_high1_10_10 > 70      4grp_highest_8_8 > 10      4grp_high_8_8 > 54      6grp_highest_nonorm > 71      6grp_total > 71      4grp_total > 57      6grp_highest_sub > 04      6grp_cgc > 84      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 315
1 2 3 4 range
A 131.2
(-1.8)
P 		297.9
(2.4)
P 		321.9
(3)
P 		84.8
(-3)
P 		237
B 137.5
(-1.6)
P 		266.8
(1.4)
P 		336.1
(3)
P 		77
(-3)
P 		259
C 130.9
(-2.5)
P 		285.4
(0.8)
P 		392.1
(3)
P 		107.1
(-3)
P 		285
avr 133.2
(-2) 		283.4
(1.5) 		350
(3) 		89.6
(-3) 		260

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 292
1 2 3 4 5 6 range
A 233.6
(-1.1)
P 		363.1
(1.5)
P 		363
(1.5)
P 		434
(3)
P 		297.9
(0.2)
P 		142.3
(-3)
P 		292
B 308.1
(1)
P 		359.4
(2.6)
P 		311.5
(1.1)
P 		372.8
(3)
P 		364.8
(2.7)
P 		181.9
(-3)
P 		191
C 365.2
(2.1)
P 		295.7
(0.2)
P 		330.5
(1.2)
P 		397.2
(3)
P 		288.3
(0)
P 		177.4
(-3)
P 		220
avr 302.3
(0.5) 		339.4
(1.4) 		335
(1.3) 		401.3
(3) 		317
(0.8) 		167.2
(-3) 		234
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 124
1 2 3 4 range
A 47.8
(-0)
P 		64.7
(1.4)
P 		84.2
(3)
P 		12.4
(-3)
P 		72
B 43.8
(-0.3)
P 		65.9
(1.8)
P 		77.9
(3)
P 		16.4
(-3)
P 		62
C 43.3
(-1.7)
P 		82.1
(0.2)
P 		136.7
(3)
P 		18.1
(-3)
P 		119
avr 45
(-0.9) 		70.9
(1) 		99.6
(3) 		15.6
(-3) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 114
1 2 3 4 5 6 range
A 43.1
(-2.5)
P 		129.1
(3)
P 		79.1
(-0.2)
P 		110.1
(1.8)
P 		89.9
(0.5)
P 		34.7
(-3)
P 		94
B 107.2
(-0.1)
P 		119.6
(0.9)
P 		75.6
(-2.4)
P 		98.7
(-0.7)
P 		148.2
(3)
P 		67.8
(-3)
P 		80
C 135.4
(3)
P 		90.2
(-0.6)
P 		84.1
(-1.1)
P 		79.9
(-1.4)
P 		93.3
(-0.3)
P 		59.4
(-3)
P 		76
avr 95.2
(1.2) 		113
(3) 		79.6
(-0.4) 		96.2
(1.3) 		110.5
(2.7) 		54
(-3) 		59
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188448_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4052
Sequence Type Exemplar sequence
Representative Public ID D1069.2 NCBI
Target Description D1069.2 /REP_DB=WormBase Gene ID /WP=CE17614 /TR=O44788 /GB=AAB94947.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=calponin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061312(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061312 NCBI Caenorhabditis elegans D1069.2 (calponin) mRNA, complete cds. 11/11 None
SNAP00000035335 ENSEMBL cdna:SNAP chromosome:CEL140:II:337337:339142:1 9/11 None
GENEFINDER00000035340 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:337800:339142:1 10/11 None
D1069.2 ENSEMBL cdna:known chromosome:CEL140:II:337337:339247:1 gene:D1069.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:337336-339142(+) 100.0 100.0
Public Domain and Genome References
Gene Title calponin
Gene Symbol cpn-2
Ensembl D1069.2
Entrez Gene 173424 Entrez gene
SwissProt O44788 EMBL-EBI
WormBase CE38495 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1583287_AT similar to neuronal protein cfa
CANINE_2:CFA.1224.1.S1_AT similar to neuronal protein cfa
CANINE_2:CFAAFFX.16241.1.S1_S_AT similar to neuronal protein cfa
DROSOPHILA_2:1630509_AT Muscle protein 20 dm
DROSGENOME1:143271_AT Muscle protein 20 dm
CHICKEN:GGA.7692.2.S1_A_AT transgelin 3 gga
HG-U95AV2:32652_G_AT transgelin 3 hs
HG-U133_PLUS_2:204743_AT transgelin 3 hs
HG-FOCUS:204743_AT transgelin 3 hs
HG-U133A:204743_AT transgelin 3 hs
HG-U133A_2:204743_AT transgelin 3 hs
HU35KSUBD:RC_F02711_AT transgelin 3 hs
U133_X3P:G10047091_3P_AT transgelin 3 hs
HG-U95AV2:32650_AT transgelin 3 hs
HG-U95AV2:32651_AT Transgelin 3 hs
MU11KSUBA:AA051560_S_AT transgelin 3 mm
MU11KSUBB:MSA.12982.0_S_AT transgelin 3 mm
MU11KSUBB:MSA.12561.0_S_AT transgelin 3 mm
MG-U74AV2:160185_AT transgelin 3 mm
MOE430A:1450683_AT transgelin 3 mm
MOUSE430A_2:1450683_AT transgelin 3 mm
MOUSE430_2:1450683_AT transgelin 3 mm
RN-U34:M84725_AT transgelin 3 rn
RG-U34A:M84725_AT transgelin 3 rn
RAE230A:1387529_A_AT transgelin 3 rn
RAT230_2:1387529_A_AT transgelin 3 rn
Protein Similarities
Method ID Description E-Value
blast NP_493713 D1069.2 [Caenorhabditis elegans] gb|AAB94947.2| Calponin protein 2 [Caenorhabditis elegans] 1.0E-115
blast NP_493713 D1069.2 [Caenorhabditis elegans] gb|AAB94947.2| Calponin protein 2 [Caenorhabditis elegans] 1.0E-114
blast CAE62871 Hypothetical protein CBG07054 [Caenorhabditis briggsae] 1.0E-112
blast NP_493713 D1069.2 [Caenorhabditis elegans] gb|AAB94947.2| Calponin protein 2 [Caenorhabditis elegans] 1.0E-104
blast CAE62871 Hypothetical protein CBG07054 [Caenorhabditis briggsae] 1.0E-102
blast AAH44160 Transgelin 2 [Danio rerio] gb|AAH66521.1| Tagln2 protein [Danio rerio] gb|AAP79113.1| transgelin [Danio rerio] ref|XP_697777.1| PREDICTED: similar to Transgelin 2 [Danio rerio] pir||JC8038 22K smooth muscle cell-specific protein SM22alpha - zebra fish 2.0E-30
Protein Domains
Database ID Description E-Value
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.3E-19
Pfam IPR000557 EMBL-EBI Calponin repeat 8.4E-11
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.3E-19
Pfam IPR000557 EMBL-EBI Calponin repeat 8.4E-11
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.3E-19
Pfam IPR000557 EMBL-EBI Calponin repeat 8.4E-11
Sequence
Target Sequence 
>C. ELEGANS:188448_AT
gttatggccaatcatggtcccagctatgggctgagtcgggaactacagaagaagaacgac
gcccgttttgtattggaggaagcaatcgaagttctaaaatggattgagaatgtaacagga
gagaggttctcatttgatgtgaccacttgtgaatcatcaactgacgtcagtaatctactc
aaggatggtgtgatgctatgcaagctgattgagaaactagatcccagttgtcgagtcgtg
tataataagaaaccaaaaatggcatttccaatgatggaaaatatttcaaatttcctggca
gctgccaaacaatttggtgttatggaaattagctgtttccagactgtggatttgtatgag
aacaaacaatgctataaggtaatcgaatgtctgcgtctcctcgccgccgtcgctcaatcc
cgttcttctcacttggagcatccagcgtgggtggtgaagcttgcccaatcatcgccacgt
caattcccggaagcagtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTATGGCCAATCATGGTCCCAGCT 109 447 205 Antisense
GGTCCCAGCTATGGGCTGAGTCGGG 233 501 220 Antisense
GACGCCCGTTTTGTATTGGAGGAAG 101 339 262 Antisense
AGGTTCTCATTTGATGTGACCACTT 658 57 328 Antisense
GACCACTTGTGAATCATCAACTGAC 390 383 345 Antisense
ACTAGATCCCAGTTGTCGAGTCGTG 576 101 420 Antisense
TTTCAAATTTCCTGGCAGCTGCCAA 483 671 488 Antisense
AGGTAATCGAATGTCTGCGTCTCCT 244 57 581 Antisense
CTTGGAGCATCCAGCGTGGGTGGTG 426 211 636 Antisense
GTGGGTGGTGAAGCTTGCCCAATCA 358 489 651 Antisense
GCCACGTCAATTCCCGGAAGCAGTG 414 279 678 Antisense
Sequence Source Affymetrix Proprietary Database