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[188443_at] cuticulin

Description T22C8.7 /REP_DB=WormBase Gene ID /WP=CE02355 /TR=Q22680 /GB=CAA88879.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cuticulin
Cluster 6grp_total > 17      4grp_total > 50      6grp_total_sub > Decrease      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 99
1 2 3 4 range
A 33.6
(-2.7)
A 		103
(3)
A 		48.4
(-1.5)
A 		30.5
(-3)
A 		73
B 47.6
(-0.8)
A 		86.4
(3)
P 		82
(2.6)
A 		24.7
(-3)
A 		62
C 17.5
(-2.1)
A 		93
(3)
P 		44.7
(-0.2)
A 		3.8
(-3)
A 		89
avr 32.9
(-1.9) 		94.1
(3) 		58.4
(0.1) 		19.7
(-3) 		74

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 102.4
(3)
A 		25.7
(-3)
A 		77.4
(1)
P 		58.6
(-0.4)
A 		64
(-0)
A 		51.6
(-1)
A 		77
B 74.4
(-1)
P 		102.5
(3)
P 		62.8
(-2.7)
A 		73.9
(-1.1)
P 		60.5
(-3)
A 		75.6
(-0.8)
A 		42
C 89.7
(3)
P 		87.1
(2.7)
P 		59.3
(-0.2)
A 		31.8
(-3)
A 		38.8
(-2.3)
P 		53.6
(-0.7)
A 		58
avr 88.8
(3) 		71.8
(0) 		66.5
(-0.9) 		54.8
(-2.9) 		54.4
(-3) 		60.3
(-2) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 26
1 2 3 4 range
A 12.2
(-0.4)
A 		22.4
(3)
A 		12.6
(-0.3)
A 		4.5
(-3)
A 		18
B 15.2
(0.7)
A 		21.3
(3)
P 		19
(2.1)
A 		5.3
(-3)
A 		16
C 5.8
(-1.8)
A 		26.7
(3)
P 		15.6
(0.4)
A 		0.6
(-3)
A 		26
avr 11.1
(-0.7) 		23.5
(3) 		15.7
(0.7) 		3.5
(-3) 		20

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 18.9
(2.8)
A 		9.1
(-3)
A 		16.9
(1.6)
P 		14.9
(0.4)
A 		19.3
(3)
A 		12.6
(-0.9)
A 		10
B 25.9
(0.4)
P 		34.1
(3)
P 		15.3
(-3)
A 		19.6
(-1.6)
P 		24.6
(-0)
A 		28.2
(1.1)
A 		19
C 33.3
(3)
P 		26.6
(1.5)
P 		15.1
(-1.1)
A 		6.4
(-3)
A 		12.6
(-1.6)
P 		17.9
(-0.4)
A 		27
avr 26
(3) 		23.3
(1.7) 		15.8
(-1.9) 		13.6
(-3) 		18.8
(-0.5) 		19.6
(-0.1) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188443_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.3034
Sequence Type Exemplar sequence
Representative Public ID T22C8.7 NCBI
Target Description T22C8.7 /REP_DB=WormBase Gene ID /WP=CE02355 /TR=Q22680 /GB=CAA88879.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=cuticulin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063434(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063434 NCBI Caenorhabditis elegans T22C8.7 (cuticulin) mRNA, complete cds. 11/11 None
SNAP00000014912 ENSEMBL cdna:SNAP chromosome:CEL140:II:8630706:8634240:-1 11/11 None
GENEFINDER00000014916 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8630706:8634237:-1 11/11 None
T22C8.7 ENSEMBL cdna:known chromosome:CEL140:II:8630706:8634237:-1 gene:T22C8.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8630702-8634234(-) 100.0 100.0
Public Domain and Genome References
Gene Title cuticulin
Ensembl T22C8.7
Entrez Gene 188735 Entrez gene
SwissProt Q22680 EMBL-EBI
WormBase CE02355 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA88879 Hypothetical protein T22C8.7 [Caenorhabditis elegans] ref|NP_495835.1| T22C8.7 [Caenorhabditis elegans] 0.0
blast CAE57551 Hypothetical protein CBG00529 [Caenorhabditis briggsae] 1.0E-168
blast CAE57551 Hypothetical protein CBG00529 [Caenorhabditis briggsae] 1.0E-164
blast CAE70602 Hypothetical protein CBG17277 [Caenorhabditis briggsae] 4.0E-52
Protein Domains
Database ID Description E-Value
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 2.5E-29
Pfam IPR001507 EMBL-EBI Endoglin/CD105 antigen 2.5E-29
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_495835.1 1 562-584
T22C8.7 1 562-584
SNAP00000014912 1 523-545
GENEFINDER00000014916 1 562-584
Sequence
Target Sequence 
>C. ELEGANS:188443_AT
attccttttctccttcgagcaaattccgaaacggctacaactttccaaacgtagaatcag
gagaaggtgttggtcaagacaacactgcagaattcggcgtcaaaagaaaatcatcagacg
caggcgtcaatgaatacggtatagaaatcgcgaaagatgagtcacaggctaaacaaaaca
agatttcacttgttgcagaacctgagaaattaccgaaacgagaagtattccacgtggaaa
caatatccaaaacaatggacagcgagctagagcctcttccgtatccaacgaaagcttctg
tataccaacatcggagtcctcgaagtcataaacgagtattggaagacgcaaatataacgt
tagaagttgaaacgacaaagattatgattttggaaaatgagccaagtgcagagaaattta
gattgggagaagctcgtgaacaatcggggccagaaccaatgaaatgtacaacatcggaaa
actatgtcagcaacactttattattgacgactttgggagtactacaggttatctcaatat
tggcgcttataactcaacgattcta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTCCTTTTCTCCTTCGAGCAAATT 472 5 1214 Antisense
ACAACACTGCAGAATTCGGCGTCAA 31 105 1292 Antisense
GACGCAGGCGTCAATGAATACGGTA 69 381 1330 Antisense
ATGGACAGCGAGCTAGAGCCTCTTC 132 53 1468 Antisense
AGCCTCTTCCGTATCCAACGAAAGC 127 77 1484 Antisense
GAAAGCTTCTGTATACCAACATCGG 591 359 1503 Antisense
CCAACATCGGAGTCCTCGAAGTCAT 47 277 1518 Antisense
GCTCGTGAACAATCGGGGCCAGAAC 618 299 1645 Antisense
GACGACTTTGGGAGTACTACAGGTT 357 287 1719 Antisense
ACAGGTTATCTCAATATTGGCGCTT 213 107 1737 Antisense
TGGCGCTTATAACTCAACGATTCTA 443 543 1754 Antisense
Sequence Source Affymetrix Proprietary Database