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[188442_at] chitinase

Description R09D1.5 /REP_DB=WormBase Gene ID /WP=CE03546 /TR=Q21861 /GB=CAA93865.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=chitinase
Cluster 6grp_total > 25      4grp_total > 63      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 12.4
(-2.8)
A 		11.6
(-3)
A 		43.4
(3)
A 		17.7
(-1.8)
A 		32
B 20.4
(3)
A 		3.9
(-2.1)
A 		1.1
(-3)
A 		3.5
(-2.3)
A 		19
C 22.1
(3)
A 		6.2
(-2)
A 		2.9
(-3)
A 		3.7
(-2.8)
A 		19
avr 18.3
(3) 		7.2
(-3) 		15.8
(1.6) 		8.3
(-2.4) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 53
1 2 3 4 5 6 range
A 22.4
(0.9)
A 		17
(-0.1)
A 		3
(-2.7)
A 		33.6
(3)
A 		1.4
(-3)
A 		3.3
(-2.6)
A 		32
B 40
(3)
A 		37.7
(2.6)
A 		13.6
(-1.2)
A 		2.5
(-2.9)
A 		2.1
(-3)
A 		15
(-1)
A 		38
C 25.9
(-0.3)
A 		54.7
(3)
A 		5.1
(-2.7)
A 		2.3
(-3)
A 		3.5
(-2.9)
A 		3.5
(-2.9)
A 		52
avr 29.4
(1.8) 		36.5
(3) 		7.2
(-2.1) 		12.8
(-1.2) 		2.3
(-3) 		7.3
(-2.1) 		34
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 4.5
(-1.6)
A 		2.5
(-3)
A 		11.3
(3)
A 		2.6
(-2.9)
A 		9
B 6.5
(3)
A 		1
(-2.3)
A 		0.3
(-3)
A 		0.7
(-2.6)
A 		6
C 7.3
(3)
A 		1.8
(-1.9)
A 		1
(-2.6)
A 		0.6
(-3)
A 		7
avr 6.1
(3) 		1.8
(-2.4) 		4.2
(0.6) 		1.3
(-3) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 16
1 2 3 4 5 6 range
A 4.1
(-0.3)
A 		6
(1.1)
A 		0.6
(-2.9)
A 		8.5
(3)
A 		0.4
(-3)
A 		0.8
(-2.7)
A 		8
B 13.9
(3)
A 		12.5
(2.4)
A 		3.3
(-1.8)
A 		0.6
(-3)
A 		0.8
(-2.9)
A 		5.6
(-0.7)
A 		13
C 9.6
(0.4)
A 		16.7
(3)
A 		1.3
(-2.7)
A 		0.5
(-3)
A 		1.1
(-2.8)
A 		1.2
(-2.7)
A 		16
avr 9.2
(1.6) 		11.7
(3) 		1.7
(-2.5) 		3.2
(-1.7) 		0.8
(-3) 		2.5
(-2.1) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188442_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2982
Sequence Type Exemplar sequence
Representative Public ID R09D1.5 NCBI
Target Description R09D1.5 /REP_DB=WormBase Gene ID /WP=CE03546 /TR=Q21861 /GB=CAA93865.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=chitinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063623(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063623 NCBI Caenorhabditis elegans R09D1.5 (chitinase) mRNA, complete cds. 11/11 None
SNAP00000010207 ENSEMBL cdna:SNAP chromosome:CEL140:II:9448185:9449578:1 11/11 None
GENEFINDER00000010219 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9448185:9449549:1 11/11 None
R09D1.5 ENSEMBL cdna:known chromosome:CEL140:II:9448185:9449578:1 gene:R09D1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9448181-9449575(+) 100.0 100.0
Public Domain and Genome References
Gene Title chitinase
Ensembl R09D1.5
Entrez Gene 187735 Entrez gene
SwissProt Q21861 EMBL-EBI
WormBase CE34961 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA93865 Hypothetical protein R09D1.5 [Caenorhabditis elegans] ref|NP_496024.2| R09D1.5 [Caenorhabditis elegans] 0.0
blast CAA93861 Hypothetical protein R09D1.1 [Caenorhabditis elegans] ref|NP_496021.1| R09D1.1 [Caenorhabditis elegans] 1.0E-102
blast CAA93861 Hypothetical protein R09D1.1 [Caenorhabditis elegans] ref|NP_496021.1| R09D1.1 [Caenorhabditis elegans] 1.0E-100
blast CAE59362 Hypothetical protein CBG02711 [Caenorhabditis briggsae] 5.0E-97
Protein Domains
Database ID Description E-Value
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 2.5E-116
Pfam IPR001223 EMBL-EBI Glycoside hydrolase, family 18 2.5E-116
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496024.2 1 28-50
R09D1.5 1 28-50
SNAP00000010207 1 43-65
GENEFINDER00000010219 1 28-50
Sequence
Target Sequence 
>C. ELEGANS:188442_AT
atggatcgtcgtcatgatggttataaatttgatgattttatggagtatattgactttgtt
aatgtattctctatggattactatggaccatggccaaaccaatggggaactcctacagga
ccatctgcaccgttgtatggaggaatcggagtaaagaaacactttaacgtggactctaca
atgaaatactatacttgtatgacagaagatccaagtaaattcaatatggtcattccattt
tatgtaagactctggaaaaatgtgaaggaaccaatctccagtggaacagaagtattccga
agagccgatctgaaaaatggtgcagccgtaggtaacagttacatgtcgcgatggacggta
gatcatgaaggttgggaattgacaccagcattgtgggatgatgttacaaagactccttat
gtctggaatcaggaaaccggaaactttttaacttttgaaaataagaaaagtattgaagct
aaacttgcgtatgcaatagagcataatttgggaggagtttggattcatttggtgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGGATCGTCGTCATGATGGTTATA 553 51 718 Antisense
TACTATGGACCATGGCCAAACCAAT 403 645 796 Antisense
ATGGGGAACTCCTACAGGACCATCT 248 51 819 Antisense
ATCTGCACCGTTGTATGGAGGAATC 63 35 840 Antisense
CACTTTAACGTGGACTCTACAATGA 364 195 877 Antisense
GAAGGAACCAATCTCCAGTGGAACA 459 331 981 Antisense
AATGGTGCAGCCGTAGGTAACAGTT 532 161 1033 Antisense
GTAACAGTTACATGTCGCGATGGAC 460 461 1049 Antisense
GGGAATTGACACCAGCATTGTGGGA 71 495 1091 Antisense
AAGACTCCTTATGTCTGGAATCAGG 341 153 1126 Antisense
GGAGGAGTTTGGATTCATTTGGTGG 260 515 1228 Antisense
Sequence Source Affymetrix Proprietary Database