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[188441_at] protease

Description F21F8.4 /REP_DB=WormBase Gene ID /WP=CE09540 /TR=O01531 /GB=AAC47994.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 18      4grp_highest_8_8 > 50      4grp_high_8_8 > 40      6grp_highest_nonorm > 30      6grp_total > 18      4grp_total > 55      6grp_highest_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5408
1 2 3 4 range
A 5543.8
(3)
P 		1110.3
(-2)
P 		1167
(-1.9)
P 		195.2
(-3)
P 		5349
B 5388.3
(3)
P 		1487.4
(-1.5)
P 		1215.1
(-1.8)
P 		136
(-3)
P 		5252
C 5167.5
(3)
P 		1486.5
(-1.5)
P 		1147.3
(-1.9)
P 		211.2
(-3)
P 		4956
avr 5366.5
(3) 		1361.4
(-1.6) 		1176.5
(-1.8) 		180.8
(-3) 		5186

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1943
1 2 3 4 5 6 range
A 2223.4
(3)
P 		826.1
(-1.5)
P 		637.5
(-2.1)
P 		536.7
(-2.4)
P 		578
(-2.3)
P 		350.4
(-3)
P 		1873
B 2106.9
(3)
P 		842.2
(-1.2)
P 		280
(-3)
P 		503.6
(-2.3)
P 		595.7
(-2)
P 		373.3
(-2.7)
P 		1827
C 1891.4
(3)
P 		854.1
(-0.9)
P 		538.3
(-2.1)
P 		356.6
(-2.8)
P 		635.2
(-1.7)
P 		295
(-3)
P 		1596
avr 2073.9
(3) 		840.8
(-1.3) 		485.3
(-2.5) 		465.6
(-2.6) 		603
(-2.1) 		339.6
(-3) 		1734
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1990
1 2 3 4 range
A 2018.5
(3)
P 		241
(-2.4)
P 		305.1
(-2.2)
P 		28.6
(-3)
P 		1990
B 1718.3
(3)
P 		367.4
(-1.8)
P 		281.6
(-2.1)
P 		29
(-3)
P 		1689
C 1710.6
(3)
P 		427.4
(-1.6)
P 		399.9
(-1.7)
P 		35.6
(-3)
P 		1675
avr 1815.8
(3) 		345.3
(-1.9) 		328.9
(-2) 		31.1
(-3) 		1785

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 665
1 2 3 4 5 6 range
A 409.9
(3)
P 		293.8
(0.9)
P 		138.8
(-2)
P 		136.1
(-2.1)
P 		174.4
(-1.4)
P 		85.3
(-3)
P 		325
B 733.1
(3)
P 		280.3
(-1.1)
P 		68
(-3)
P 		133.4
(-2.4)
P 		242
(-1.4)
P 		139.2
(-2.4)
P 		665
C 701.1
(3)
P 		260.6
(-1.2)
P 		137
(-2.4)
P 		71.7
(-3)
P 		205.5
(-1.7)
P 		98.7
(-2.7)
P 		629
avr 614.7
(3) 		278.2
(-1) 		114.6
(-2.9) 		113.7
(-2.9) 		207.3
(-1.8) 		107.7
(-3) 		507
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188441_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12283
Sequence Type Exemplar sequence
Representative Public ID F21F8.4 NCBI
Target Description F21F8.4 /REP_DB=WormBase Gene ID /WP=CE09540 /TR=O01531 /GB=AAC47994.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072733(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072733 NCBI Caenorhabditis elegans F21F8.4 (protease) mRNA, complete cds. 11/11 None
SNAP00000008546 ENSEMBL cdna:SNAP chromosome:CEL140:V:8273235:8274598:1 11/11 None
GENEFINDER00000008566 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8273235:8274598:1 11/11 None
F21F8.4.1 ENSEMBL cdna:known chromosome:CEL140:V:8271844:8274635:1 gene:F21F8.4 11/11 None
F21F8.4.2 ENSEMBL cdna:known chromosome:CEL140:V:8273233:8274598:1 gene:F21F8.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8273235-8274599(+) 100.0 100.0
Public Domain and Genome References
Gene Title protease
Ensembl F21F8.4
Entrez Gene 179210 Entrez gene
SwissProt O01531 EMBL-EBI
WormBase CE09540 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4190 aspartic-type endopeptidase activity inferred from electronic annotation QuickGO AmiGO
4194 pepsin A activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC47994 Hypothetical protein F21F8.4 [Caenorhabditis elegans] ref|NP_505134.1| F21F8.4 [Caenorhabditis elegans] 0.0
blast AAC47991 Aspartyl protease protein 5 [Caenorhabditis elegans] ref|NP_505135.1| F21F8.3 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 1.5E-67
Sequence
Target Sequence 
>C. ELEGANS:188441_AT
ttcacatctcttgcagtcgacggagttaccccaccactgatcaacgccatcaacaagaaa
attctcgatcaacctctgttcacagtctttttagaacataagggaacggcaaagaatgtt
ggaggaggtgtcttcacctacggagccgttgacactaccaactgcggaccagtgattgcc
tatcaaccactttcatctgccacgtattatcagtttgttgctgatggatttaagctcgga
tcatactcaaacaataagaaatctcaagtgatttccgacactggaagttcgctcatcgga
ggaccaagtgctgtgataaatggcttcgctcaagctttgggagcaaaataccacagcgat
gatgatgcttactacctcccatgctcaacatctaagggaactttggacattactatcgga
ggaaatgtgtattctattgaacctgtcaactatatccttgatgttggaatgggtgacact
tgtgtctttgctgctttctcattcgatttcggaggattcggaccatcatggattttcgga
gatcctttcatccgtcaatactgcaacatctatgacatcggacaacagcgcattggtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCACATCTCTTGCAGTCGACGGAG 40 685 571 Antisense
GATCAACCTCTGTTCACAGTCTTTT 51 423 637 Antisense
CTACGGAGCCGTTGACACTACCAAC 653 225 708 Antisense
ACTTTCATCTGCCACGTATTATCAG 342 103 759 Antisense
TGGAAGTTCGCTCATCGGAGGACCA 555 545 852 Antisense
AAATGGCTTCGCTCAAGCTTTGGGA 197 121 888 Antisense
TACCTCCCATGCTCAACATCTAAGG 305 647 943 Antisense
TGACACTTGTGTCTTTGCTGCTTTC 453 571 1044 Antisense
GCTGCTTTCTCATTCGATTTCGGAG 457 297 1060 Antisense
TTTTCGGAGATCCTTTCATCCGTCA 394 677 1103 Antisense
GACATCGGACAACAGCGCATTGGTT 488 369 1144 Antisense
Sequence Source Affymetrix Proprietary Database