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[188435_at] protease

Description F21F8.2 /REP_DB=WormBase Gene ID /WP=CE09538 /TR=O01533 /GB=AAC47992.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster 6grp_total > 56      4grp_total > 14      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 26.9
(-0.2)
A 		2.3
(-3)
A 		2.7
(-3)
A 		55.7
(3)
A 		53
B 39.2
(3)
A 		2.9
(-3)
A 		5.4
(-2.6)
A 		14.9
(-1)
A 		36
C 33.1
(-0.4)
A 		4.5
(-3)
A 		4.8
(-3)
A 		71.1
(3)
P 		67
avr 33.1
(1.1) 		3.2
(-3) 		4.3
(-2.9) 		47.2
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 107
1 2 3 4 5 6 range
A 10.1
(-3)
A 		47
(-0.8)
M 		77.9
(1.1)
P 		16.7
(-2.6)
A 		13.6
(-2.8)
A 		109.7
(3)
P 		100
B 46.8
(0)
A 		67.5
(1.4)
A 		39.8
(-0.5)
A 		3.1
(-3)
A 		67.2
(1.4)
P 		90
(3)
P 		87
C 28.9
(-2.6)
A 		90.7
(3)
P 		23.9
(-3)
A 		54.8
(-0.2)
A 		27.3
(-2.7)
A 		64.7
(0.7)
P 		67
avr 28.6
(-2.6) 		68.4
(1.1) 		47.2
(-0.9) 		24.9
(-3) 		36
(-1.9) 		88.1
(3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 9.8
(3)
A 		0.5
(-3)
A 		0.7
(-2.9)
A 		8.2
(2)
A 		9
B 12.5
(3)
A 		0.7
(-3)
A 		1.2
(-2.7)
A 		3.2
(-1.7)
A 		12
C 11
(2.4)
A 		1.3
(-3)
A 		1.7
(-2.8)
A 		12
(3)
P 		11
avr 11.1
(3) 		0.8
(-3) 		1.2
(-2.8) 		7.8
(1.1) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 33
1 2 3 4 5 6 range
A 1.9
(-3)
A 		16.7
(0.6)
M 		17
(0.7)
P 		4.2
(-2.4)
A 		4.1
(-2.5)
A 		26.7
(3)
P 		25
B 16.3
(-0.2)
A 		22.5
(1)
A 		9.7
(-1.4)
A 		0.8
(-3)
A 		27.3
(1.8)
P 		33.6
(3)
P 		33
C 10.7
(-1.7)
A 		27.7
(3)
P 		6.1
(-3)
A 		11
(-1.6)
A 		8.8
(-2.3)
A 		21.7
(1.3)
P 		22
avr 9.6
(-1.8) 		22.3
(1.6) 		10.9
(-1.5) 		5.3
(-3) 		13.4
(-0.8) 		27.3
(3) 		22
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 188435_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11261
Sequence Type Exemplar sequence
Representative Public ID F21F8.2 NCBI
Target Description F21F8.2 /REP_DB=WormBase Gene ID /WP=CE09538 /TR=O01533 /GB=AAC47992.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_072735(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_072735 NCBI Caenorhabditis elegans F21F8.2 (protease) mRNA, complete cds. 9/11 None
SNAP00000008548 ENSEMBL cdna:SNAP chromosome:CEL140:V:8277182:8278347:1 10/11 None
GENEFINDER00000008563 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:8274977:8278347:1 11/11 None
F21F8.2 ENSEMBL cdna:known chromosome:CEL140:V:8277209:8278347:1 gene:F21F8.2 9/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:8277182-8278348(+) 100.0 100.0
Public Domain and Genome References
Gene Title protease
Ensembl F21F8.2
Entrez Gene 184787 Entrez gene
SwissProt O01533 EMBL-EBI
WormBase CE35284 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4194 pepsin A activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAC47991 Aspartyl protease protein 5 [Caenorhabditis elegans] ref|NP_505135.1| F21F8.3 [Caenorhabditis elegans] 0.0
blast CAE66086 Hypothetical protein CBG11303 [Caenorhabditis briggsae] 0.0
blast AAC47994 Hypothetical protein F21F8.4 [Caenorhabditis elegans] ref|NP_505134.1| F21F8.4 [Caenorhabditis elegans] 1.0E-172
blast CAE66085 Hypothetical protein CBG11302 [Caenorhabditis briggsae] 4.0E-85
blast AAC47992 Hypothetical protein F21F8.2 [Caenorhabditis elegans] ref|NP_505136.2| F21F8.2 [Caenorhabditis elegans] 1.0E-73
blast AAC47992 Hypothetical protein F21F8.2 [Caenorhabditis elegans] ref|NP_505136.2| F21F8.2 [Caenorhabditis elegans] 2.0E-72
blast CAE66085 Hypothetical protein CBG11302 [Caenorhabditis briggsae] 9.0E-53
blast AAC47991 Aspartyl protease protein 5 [Caenorhabditis elegans] ref|NP_505135.1| F21F8.3 [Caenorhabditis elegans] 1.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 6.0E-19
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 5.3E-19
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 1.7E-65
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 1.3E-7
Pfam IPR001461 EMBL-EBI Peptidase A1, pepsin 4.2E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_505136.2 2 23-45,89-111
F21F8.2 2 23-45,89-111
SNAP00000008548 2 107-129,173-195
GENEFINDER00000008563 1 5-22
Sequence
Target Sequence 
>C. ELEGANS:188435_AT
ggtcttgcaccttcaatgaactatgatcgagtattcttatctccacttttaaatgcaatt
caacaaggactactagacgatccactttttactgtttatctggaaaagcatggttttacg
agcaatgttccaggtggaagatttacctatggagcagttgatattgataactgcgggcct
attgatggtgtttctattggaaattacacgttaactcggaaaatgcaagtcatgactgac
acgggaacttcgttcattgctgccccattctatgtgctcaatgaaattattgtaaccctt
ggagcaacttacgataacacgacaaaaagctattatgtttcttgtaattctgagaaacca
aatttgaacattgttatcggaaataacatctatgttgttgagcccaataattatgttatt
gatgtaagtacagaaaagattcagagaaacataactaccgcaccaataactataaaccaa
ggtgacaaatgcctgttggctatcagtggacttagtggatacggtatgggtcctacatgg
attttgggaacactattcatgagacagttctgtcatgttcacgac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGTCTTGCACCTTCAATGAACTATG 564 503 88 Antisense
GATCGAGTATTCTTATCTCCACTTT 629 417 112 Antisense
GACTACTAGACGATCCACTTTTTAC 207 371 155 Antisense
GGTTTTACGAGCAATGTTCCAGGTG 669 505 199 Antisense
GCGGGCCTATTGATGGTGTTTCTAT 74 293 260 Antisense
CACGGGAACTTCGTTCATTGCTGCC 528 197 327 Antisense
GTAACCCTTGGAGCAACTTACGATA 176 461 379 Antisense
GAAACATAACTACCGCACCAATAAC 475 355 533 Antisense
ATGCCTGTTGGCTATCAGTGGACTT 405 41 576 Antisense
GATACGGTATGGGTCCTACATGGAT 102 423 605 Antisense
GAGACAGTTCTGTCATGTTCACGAC 119 393 648 Antisense
Sequence Source Affymetrix Proprietary Database